The Metabolomic Approach to the Diagnosis of Critical Illness
Advances in molecular and cell biology over the last two decades, including most notably the sequencing of the human genome, have undoubtedly determined a better understanding of disease pathophysiology, and a more precise identification of populations at risk for certain conditions. However, many questions remain unanswered using the genomic approach alone, including what the gene products and the cell responses to certain insults are, given a certain genetic abnormality. The study of the metabolome offers a unique opportunity to answer some of these questions. The metabolome represents a combination of all the metabolites and intermediate products of metabolism in a biological organism. The study of the metabolome, further down the line from gene structure and function, more closely reflects the activities of the cell at the functional level and magnifies events that occur at the level of the genome, transcriptome and proteome (Fig. 1).
KeywordsHigh Performance Liquid Chromatography Acute Lung Injury Critical Illness Exhale Breath Condensate Metabolomic Analysis
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