Antimicrobial Nanomaterials for Water Disinfection

  • Chong Liu
  • Xing Xie
  • Yi Cui


Water treatment is important to protect people from water-borne diseases. Traditional chemical disinfection creates a lot of disinfection by-products which are harmful to human health. Thus, there is a growing need for new water disinfection methods to effectively move pathogens from water sources. Nanotechnology has the potential to meet the challenge to provide new water treatment methods. In this chapter, we introduce several antimicrobial nanomaterials including oligodynamic metals (e.g., nAg), photocatalytic semiconductors (e.g., TiO2), and carbon nanomaterials (e.g., CNT) and their disinfection mechanisms. Moreover, we use cases to illustrate how these materials are designed and used in real water treatment devices.


Antimicrobial Effect Effluent Water Water Disinfection Ceramic Filter Hybrid Electrode 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    UNESCO, Water a shared responsibility, United Nations Educational, Scientific and Cultural Organization. Scientific and Cultural Organization. Paris, France: UNESCO; New York: Berghahn Books, 2006. p. 20–24.Google Scholar
  2. 2.
    Wolff, G., et al., The biennial report on freshwater resources, Washington, D.C., in the world’s water 2006–2007 the biennial report on freshwater resources. Washington, DC: Island Press, 2006. p. 1–28.Google Scholar
  3. 3.
    WHO, Water, sanitation and hygiene links to health. 2004, World Health Organization.
  4. 4.
    Li, Q., et al., Antimicrobial nanomaterials for water disinfection and microbial control: Potential applications and implications. Water Research, 2008. 42(18): p. 4591–4602.CrossRefGoogle Scholar
  5. 5.
    Diallo, M., et al., Nanotechnology applications for clean water. Norwich, NY: William Andrew, 2009. p. 3–15.Google Scholar
  6. 6.
    Nangmenyi, G. and J. Economy, Nanometallic particles for oligodynamic microbial disinfection, in Nmotechnology Applications for Clean Water, N. Savage, et al., Editors. 2009, William Andrew Inc.: Norwich. p. 3–15.CrossRefGoogle Scholar
  7. 7.
    Chong, M.N., et al., Recent developments in photocatalytic water treatment technology: A review. Water Research, 2010. 44(10): p. 2997–3027.CrossRefGoogle Scholar
  8. 8.
    Gondal, M.A., M.A. Dastageer, and A. Khalil, Synthesis of nano-WO3 and its catalytic activity for enhanced antimicrobial process for water purification using laser induced photo-catalysis. Catalysis Communications, 2009. 11(3): p. 214–219.CrossRefGoogle Scholar
  9. 9.
    Lee, J.S., et al., Photochemical and antimicrobial properties of novel C60 derivatives in aqueous systems. Environmental Science & Technology, 2009. 43(17): p. 6604–6610.CrossRefGoogle Scholar
  10. 10.
    Kang, S., et al., Single-walled carbon nanotubes exhibit strong antimicrobial activity. Langmuir, 2007. 23(17): p. 8670–8673.CrossRefGoogle Scholar
  11. 11.
    Magrez, A., et al., Cellular toxicity of carbon-based nanomaterials. Nano Letters, 2006. 6(6): p. 1121–1125.CrossRefGoogle Scholar
  12. 12.
    Russell, A.D. and W.B. Hugo, Antimicrobial activity and action of silver. Progress in Medicinal Chemistry, 1994. 31: p. 351–370.CrossRefGoogle Scholar
  13. 13.
    Jung, W.K., et al., Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Applied and Environmental Microbiology, 2008. 74(7): p. 2171–2178.CrossRefGoogle Scholar
  14. 14.
    Inoue, Y., et al., Bactericidal activity of Ag-zeolite mediated by reactive oxygen species under aerated conditions. Journal of Inorganic Biochemistry, 2002. 92(1): p. 37–42.CrossRefGoogle Scholar
  15. 15.
    Li, W.R., et al., Antibacterial effect of silver nanoparticles on Staphylococcus aureus. Biometals, 2011. 24(1): p. 135–141.CrossRefGoogle Scholar
  16. 16.
    Morones, J.R., et al., The bactericidal effect of silver nanoparticles. Nanotechnology, 2005. 16(10): p. 2346–2353.CrossRefGoogle Scholar
  17. 17.
    Pal, S., Y.K. Tak, and J.M. Song, Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Applied and Environmental Microbiology, 2007. 73(6): p. 1712–1720.CrossRefGoogle Scholar
  18. 18.
    Gogoi, S.K., et al., Green fluorescent protein-expressing Escherichia coli as a model system for investigating the antimicrobial activities of silver nanoparticles. Langmuir, 2006. 22(22): p. 9322–9328.CrossRefGoogle Scholar
  19. 19.
    Larimer, C., et al., The segregation of silver nanoparticles in low-cost ceramic water filters. Materials Characterization, 2010. 61(4): p. 408–412.CrossRefGoogle Scholar
  20. 20.
    Yaohui, L., et al., Silver nanoparticle-decorated porous ceramic composite for water treatment. Journal of Membrane Science, 2009. 331(1–2): p. 50–56.CrossRefGoogle Scholar
  21. 21.
    van Halem, D., et al., Assessing the sustainability of the silver-impregnated ceramic pot filter for low-cost household drinking water treatment. Physics and Chemistry of the Earth, 2009. 34(1–2): p. 36–42.Google Scholar
  22. 22.
    Oyanedel-Craver, V.A. and J.A. Smith, Sustainable colloidal-silver-impregnated ceramic filter for point-of-use water treatment. Environmental Science & Technology, 2008. 42(3): p. 927–933.CrossRefGoogle Scholar
  23. 23.
    Yang, L., et al., Development and characterization of porous silver-incorporated hydroxyapatite ceramic for separation and elimination of microorganisms. Journal of Biomedical Materials Research Part B-Applied Biomaterials, 2007. 81B(1): p. 50–56.CrossRefGoogle Scholar
  24. 24.
    Halem, D.v., et al., Ceramic silver-impregnated pot filters for household drinking water treatment in developing countries: Material characterization and performance study. Water Science and Technology: Water Supply, 2007. 7(5–6): p. 9–17.Google Scholar
  25. 25.
    Nangmenyi, G., et al., Synthesis and characterization of silver-nanoparticle-impregnated fiberglass and utility in water disinfection. Nanotechnology, 2009. 20(49): p. 1–10.Google Scholar
  26. 26.
    Nangmenyi, G., et al., Bactericidal activity of Ag nanoparticle-impregnated fibreglass for water disinfection. Journal of Water and Health, 2009. 7(4): p. 657–663.CrossRefGoogle Scholar
  27. 27.
    Zhang, X.L., et al., Immobilizing silver nanoparticles onto the surface of magnetic silica composite to prepare magnetic disinfectant with enhanced stability and antibacterial activity. Colloids and Surfaces a-Physicochemical and Engineering Aspects, 2011. 375(1–3): p. 186–192.CrossRefGoogle Scholar
  28. 28.
    Gangadharan, D., et al., Polymeric microspheres containing silver nanoparticles as a bactericidal agent for water disinfection. Water Research, 2010. 44(18): p. 5481–5487.CrossRefGoogle Scholar
  29. 29.
    De Gusseme, B., et al., Virus disinfection in water by biogenic silver immobilized in polyvinylidene fluoride membranes. Water Research, 2011. 45(4): p. 1856–1864.CrossRefGoogle Scholar
  30. 30.
    Dankovich, T.A. and D.G. Gray, Bactericidal paper impregnated with silver nanoparticles for point-of-use water treatment. Environmental Science & Technology, 2011. 45(5): p. 1992–1998.CrossRefGoogle Scholar
  31. 31.
    Panyala, N.R., E.M. Pena-Mendez, and J. Havel, Silver or silver nanoparticles: A hazardous threat to the environment and human health? Journal of Applied Biomedicine, 2008. 6(3): p. 117–129.Google Scholar
  32. 32.
    USEPA, National secondary drinking water regulations. 2002. p. 6.
  33. 33.
    Silver, S., Bacterial silver resistance: Molecular biology and uses and misuses of silver compounds. FEMS Microbiology Reviews, 2003. 27(2–3): p. 341–353.CrossRefGoogle Scholar
  34. 34.
    Matsunaga, T., et al., Photoelectrochemical sterilization of microbial-cells by semiconductor powders. FEMS Microbiology Letters, 1985. 29(1–2): p. 211–214.CrossRefGoogle Scholar
  35. 35.
    Kikuchi, Y., et al., Photocatalytic bactericidal effect of TiO2 thin films: Dynamic view of the active oxygen species responsible for the effect. Journal of Photochemistry and Photobiology a-Chemistry, 1997. 106(1–3): p. 51–56.CrossRefGoogle Scholar
  36. 36.
    Adams, L.K., et al., Comparative toxicity of nano-scale TiO2, SiO2 and ZnO water suspensions. Water Science and Technology, 2006. 54(11–12): p. 327–334.CrossRefGoogle Scholar
  37. 37.
    Collins-Martinez, V., A.L. Ortiz, and A.A. Elguezabal, Influence of the anatase/rutile ratio on the TiO2 photocatalytic activity for the photodegradation of light hydrocarbons. International Journal of Chemical Reactor Engineering, 2007. 5: A29.Google Scholar
  38. 38.
    Page, K., et al., Titania and silver-titania composite films on glass-potent antimicrobial coatings. Journal of Materials Chemistry, 2007. 17(1): p. 95–104.CrossRefGoogle Scholar
  39. 39.
    Kim, J.P., et al., Manufacturing of anti-viral inorganic materials from colloidal silver and titanium oxide. Revue Roumaine De Chimie, 2006. 51(11): p. 1121–1129.Google Scholar
  40. 40.
    Wei, C., et al., Bactericidal activity of TiO2 photocatalyst in aqueous-media - Toward a solar-assisted water disinfection system. Environmental Science & Technology, 1994. 28(5): p. 934–938.CrossRefGoogle Scholar
  41. 41.
    Reed, R.H., The inactivation of microbes by sunlight: Solar disinfection as a water treatment process. Advances in Applied Microbiology, Vol 54, 2004. 54: p. 333–365.CrossRefGoogle Scholar
  42. 42.
    Blanco, J., et al., Review of feasible solar energy applications to water processes. Renewable & Sustainable Energy Reviews, 2009. 13(6–7): p. 1437–1445.CrossRefGoogle Scholar
  43. 43.
    Chong, M.N., et al., Optimisation of an annular photoreactor process for degradation of Congo Red using a newly synthesized titania impregnated kaolinite nano-photocatalyst. Separation and Purification Technology, 2009. 67(3): p. 355–363.CrossRefGoogle Scholar
  44. 44.
    Belhacova, L., et al., Inactivation of microorganisms in a flow-through photoreactor with an immobilized TiO2 layer. Journal of Chemical Technology and Biotechnology, 1999. 74(2): p. 149–154.CrossRefGoogle Scholar
  45. 45.
    Chan, A.H.C., et al., Solar photocatalytic thin film cascade reactor for treatment of benzoic acid containing wastewater. Water Research, 2003. 37(5): p. 1125–1135.CrossRefGoogle Scholar
  46. 46.
    Fernandez-Ibanez, P., et al., Application of the colloidal stability of TiO2 particles for recovery and reuse in solar photocatalysis. Water Research, 2003. 37(13): p. 3180–3188.CrossRefGoogle Scholar
  47. 47.
    Doll, T.E. and F.H. Frimmel, Cross-flow microfiltration with periodical back-washing for photocatalytic degradation of pharmaceutical and diagnostic residues-evaluation of the long-term stability of the photocatalytic activity of TiO2. Water Research, 2005. 39(5): p. 847–854.CrossRefGoogle Scholar
  48. 48.
    Zhang, X.W., et al., TiO2 nanowire membrane for concurrent filtration and photocatalytic oxidation of humic acid in water. Journal of Membrane Science, 2008. 313(1–2): p. 44–51.CrossRefGoogle Scholar
  49. 49.
    Zhao, Y.J., et al., Fouling and regeneration of ceramic microfiltration membranes in processing acid wastewater containing fine TiO2 particles. Journal of Membrane Science, 2002. 208(1–2): p. 331–341.CrossRefGoogle Scholar
  50. 50.
    Lee, D.K., et al., Photocatalytic oxidation of microcystin-LR in a fluidized bed reactor having TiO2-coated activated carbon. Separation and Purification Technology, 2004. 34(1–3): p. 59–66.CrossRefGoogle Scholar
  51. 51.
    Li, Y.J., M.Y. Ma, and X.H. Wang, Inactivated properties of activated carbon-supported TiO2 nanoparticles for bacteria and kinetic study. Journal of Environmental Sciences-China, 2008. 20(12): p. 1527–1533.CrossRefGoogle Scholar
  52. 52.
    Chong, M.N., et al., Synthesis and characterisation of novel titania impregnated kaolinite nano-photocatalyst. Microporous and Mesoporous Materials, 2009. 117(1–2): p. 233–242.CrossRefGoogle Scholar
  53. 53.
    Zhu, H.Y., et al., Hydrogen titanate nanofibers covered with anatase nanocrystals: A delicate structure achieved by the wet chemistry reaction of the titanate nanofibers. Journal of the American Chemical Society, 2004. 126(27): p. 8380–8381.CrossRefGoogle Scholar
  54. 54.
    Kwak, S.Y., S.H. Kim, and S.S. Kim, Hybrid organic/inorganic reverse osmosis (RO) membrane for bactericidal anti-fouling. 1. Preparation and characterization of TiO2 nanoparticle self-assembled aromatic polyamide thin-film-composite (TFC) membrane. Environmental Science & Technology, 2001. 35(11): p. 2388–2394.CrossRefGoogle Scholar
  55. 55.
    Tang, C. and V. Chen, The photocatalytic degradation of reactive black 5 using TiO2/UV in an annular photoreactor. Water Research, 2004. 38(11): p. 2775–2781.MathSciNetCrossRefGoogle Scholar
  56. 56.
    Gelover, S., et al., A practical demonstration of water disinfection using TiO2 films and sunlight. Water Research, 2006. 40(17): p. 3274–3280.CrossRefGoogle Scholar
  57. 57.
    Jia, G., et al., Cytotoxicity of carbon nanomaterials: Single-wall nanotube, multi-wall nanotube, and fullerene. Environmental Science & Technology, 2005. 39(5): p. 1378–1383.CrossRefGoogle Scholar
  58. 58.
    Kang, S., et al., Antibacterial effects of carbon nanotubes: Size does matter. Langmuir, 2008. 24(13): p. 6409–6413.CrossRefGoogle Scholar
  59. 59.
    Narayan, R.J., C.J. Berry, and R.L. Brigmon, Structural and biological properties of carbon nanotube composite films. Materials Science and Engineering B-Solid State Materials for Advanced Technology, 2005. 123(2): p. 123–129.CrossRefGoogle Scholar
  60. 60.
    Markovic, Z., et al., The mechanism of cell-damaging reactive oxygen generation by colloidal fullerenes. Biomaterials, 2007. 28(36): p. 5437–5448.CrossRefGoogle Scholar
  61. 61.
    Hu, L.B., D.S. Hecht, and G. Gruner, Carbon nanotube thin tilms: Fabrication, properties, and applications. Chemical Reviews, 2010. 110(10): p. 5790–5844.CrossRefGoogle Scholar
  62. 62.
    Schoen, D.T., et al., High speed water sterilization using one-dimensional nanostructures. Nano Letters, 2010. 10(9): p. 3628–3632.MathSciNetCrossRefGoogle Scholar
  63. 63.
    Vecitis, C.D., et al., Electrochemical multiwalled carbon nanotube filter for viral and bacterial removal and inactivation. Environmental Science & Technology, 2011. 45 (8): pp. 3672–3679.CrossRefGoogle Scholar
  64. 64.
    Brady-Estevez, A.S., S. Kang, and M. Elimelech, A single-walled-carbon-nanotube filter for removal of viral and bacterial pathogens. Small, 2008. 4(4): p. 481–484.CrossRefGoogle Scholar
  65. 65.
    Heymann, D., Solubility of fullerenes C-60 and C-70 in seven normal alcohols and their deduced solubility in water. Fullerene Science and Technology, 1996. 4(3): p. 509–515.CrossRefGoogle Scholar
  66. 66.
    Spesia, M.B., A.E. Milanesio, and E.N. Durantini, Synthesis, properties and photodynamic inactivation of Escherichia coli by novel cationic fullerene C-60 derivatives. European Journal of Medicinal Chemistry, 2008. 43(4): p. 853–861.CrossRefGoogle Scholar
  67. 67.
    Brant, J.A., et al., Characterizing the impact of preparation method on fullerene cluster structure and chemistry. Langmuir, 2006. 22(8): p. 3878–3885.CrossRefGoogle Scholar
  68. 68.
    Fortner, J.D., et al., C-60 in water: Nanocrystal formation and microbial response. Environmental Science & Technology, 2005. 39(11): p. 4307–4316.CrossRefGoogle Scholar
  69. 69.
    Zhang, D., G. Li, and J.C. Yu, Inorganic materials for photocatalytic water disinfection. Journal of Materials Chemistry, 2010. 20(22): p. 4529.CrossRefGoogle Scholar
  70. 70.
    Hayden, S.C., N.K. Allam, and M.A. El-Sayed, TiO2 nanotube/CdS hybrid electrodes: Extraordinary enhancement in the inactivation of Escherichia coli. Journal of the American Chemical Society, 2010. 132(41): p. 14406–14408.CrossRefGoogle Scholar
  71. 71.
    Kang, Q., et al., A ternary hybrid CdS/Pt-TiO2 nanotube structure for photoelectrocatalytic bactericidal effects on Escherichia coli. Biomaterials, 2010. 31(12): p. 3317–3326.CrossRefGoogle Scholar
  72. 72.
    Baram, N., et al., Enhanced inactivation of E. coli bacteria using immobilized porous TiO2 photoelectrocatalysis. Electrochimica Acta, 2009. 54(12): p. 3381–3386.CrossRefGoogle Scholar
  73. 73.
    Allam, N.K. and C.A. Grimes, Effect of cathode material on the morphology and photoelectrochemical properties of vertically oriented TiO2 nanotube arrays. Solar Energy Materials and Solar Cells, 2008. 92(11): p. 1468–1475.CrossRefGoogle Scholar
  74. 74.
    Allam, N.K., K. Shankar, and C.A. Grimes, Photoelectrochemical and water photoelectrolysis properties of ordered TiO2 nanotubes fabricated by Ti anodization in fluoride-free HCl electrolytes. Journal of Materials Chemistry, 2008. 18(20): p. 2341.CrossRefGoogle Scholar
  75. 75.
    Spadaro, J.A., et al., Antibacterial effects of silver electrodes with weak direct current. Antimicroblal Agents and Chemotherapy, 1974. 6(5): p. 637–642.Google Scholar
  76. 76.
    Akhavan, O. and E. Ghaderi, Enhancement of antibacterial properties of Ag nanorods by electric field. Science and Technology of Advanced Materials, 2009. 10(1): p. 015003.CrossRefGoogle Scholar
  77. 77.
    Tsong, T.Y., Electroporation of cell-membranes. Biophysical Journal, 1991. 60: p. 297–306.CrossRefGoogle Scholar
  78. 78.
    Li, X.Y., et al., Electrochemical disinfection of saline wastewater effluent. Journal of Environmental Engineering, 2002. 128(8): p. 697–704.CrossRefGoogle Scholar
  79. 79.
    He, J., T. Kunitake, and A. Nakao, Facile in situ synthesis of noble metal nanoparticles in porous cellulose fibers. Chemistry of Materials, 2003. 15(23): p. 4401–4406.CrossRefGoogle Scholar
  80. 80.
    Maneerung, T., S. Tokura, and R. Rujiravanit, Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydrate Polymers, 2008. 72(1): p. 43–51.CrossRefGoogle Scholar
  81. 81.
    Cates, E.L., M. Cho, and J.-H. Kim, Converting visible light into UVC: Microbial inactivation by Pr3 + −activated upconversion materials. Environmental Science & Technology, 2011, 45(8): p. 3680–3686. doi:  10.1021/es200196c
  82. 82.
    Nicholson, W.L. and B. Galeano, UV resistance of Bacillus anthracis spores revisited: Validation of Bacillus subtilis spores as UV surrogates for spores of B. anthracis sterne. Applied and Environmental Microbiology, 2003. 69(2): p. 1327–1330.CrossRefGoogle Scholar

Copyright information

© Springer Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Material Science and EngineeringStanford UniversityStanfordUSA
  2. 2.Civil and Environmental EngineeringStanford UniversityStanfordUSA

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