Ex Vivo Magnetic Sentinel Lymph Node Detection in Colorectal Cancer with a SPIO Tracer

  • Joost J. PouwEmail author
  • Raluca M. Fratila
  • Aldrik H. Velders
  • Bennie ten Haken
  • Quentin A. Pankhurst
  • Joost M. Klaase
Part of the Springer Proceedings in Physics book series (SPPHY, volume 140)


A new method for selecting sentinel lymph nodes (SNs) in colorectal cancer tissue was investigated in 12 patients. A tracer consisting of superparamagnetic ironoxide (SPIO) nanoparticles was injected in the resected tissue. A handheld magnetic probe was used to select SNs to which the SPIO was drained. Vibrating sample magnetometry was performed on the lymph nodes to quantify the amount of SPIO in the nodes. High-field MRI allowed to depict the distribution of SPIO in the node, and revealed small anatomical structures. One or more SPIO containing nodes were successfully selected with the magnetic probe in all 12 patients.


Colorectal Cancer Sentinel Lymph Node Prussian Blue Sentinel Lymph Node Mapping Sentinel Lymph Node Detection 
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Copyright information

© Springer-Verlag GmbH Berlin Heidelberg 2012

Authors and Affiliations

  • Joost J. Pouw
    • 1
    Email author
  • Raluca M. Fratila
    • 1
  • Aldrik H. Velders
    • 2
  • Bennie ten Haken
    • 1
  • Quentin A. Pankhurst
    • 3
  • Joost M. Klaase
    • 4
  1. 1.MIRA Institute for Biomedical Technology & Technical Medicine, NeuroImaging GroupUniversity of TwenteEnschedeThe Netherlands
  2. 2.Biomedical Chemistry GroupUniversity of TwenteEnschedeThe Netherlands
  3. 3.Davy-Faraday Research LaboratoryThe Royal Institution of Great BritainLondonUK
  4. 4.Department of SurgeryMedisch Spectrum TwenteEnschedeThe Netherlands

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