In Vitro Antioxidant and Cytotoxicity Assay of Pistia stratiotes L. Against B16F1 and B16F10 Melanoma Cell Lines

  • M. Jha
  • V. Sharma
  • N. Ganesh

Abstract

In this study we investigated in vitro antioxidant activity and tumor growth inhibition by Pistia stratiotes L. on melanoma cell lines. The methanolic extract of Pistia stratiotes showed that percentage inhibition of DPPH increases with the increasing concentrations of test sample. The percentage inhibition of MEPS was (17.24– 79.3 %) on DPPH against reference ascorbic acid (22.7–83.4 %) ranges (10–100 μg/ml). The effect of MEPS on the proliferation of B16F1 and B16F10 melanoma cell lines was determined by MTT and TBE bioassay. Among the two cell lines studied, the extract exhibited maximum anticancer activity with IC50 (5.09). Structural elucidation of its bioactive principle is in progress.

Keywords

Methanolic Extract Melanoma Cell Line DPPH Assay Pistia Stratiotes B16F10 Murine Melanoma Cell 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • M. Jha
    • 1
  • V. Sharma
    • 2
  • N. Ganesh
    • 1
  1. 1.Department of ResearchJawaharlal Nehru Cancer Hospital & Research CenterIdgah Hills, BhopalIndia
  2. 2.Department of ZoologyDr. Hari Singh Gaur UniversitySagarIndia

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