Advertisement

Statistical Media Optimization for Enhanced Biomass and Artemisinin Production in Artemisia Annua Hairy Roots

  • N. Patra
  • S. Sharma
  • A. K. Srivastava

Abstract

Artemisinin is an effective anti-malarial drug which is widely used to cure multi-drug resistant malaria caused by Plasmodium falciparum. Production via hairy root cultivation of plant cell lines features an attractive alternative for in-vitro mass production of artemisinin. Identification of appropriate medium recipe for growth and product formation is extremely important for increased biomass and /or Artemisinin production. Statistical media design was used to establish optimal concentration of the media components in the cultivation medium. Using optimized media it was possible to enhance the biomass concentration in the shake flask hairy root cultivation to 5.70 g/L and Artemisinin content to 1.9 mg/g in 15d. These values are significantly greater than the corresponding values from the un-optimized medium.

Keywords

Hairy Root Central Composite Design Cerebral Malaria Hairy Root Cultivation Artemisinin Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    S. Srivastava, A.K. Srivastava; Critical Reviews in Biotechnology 27 (2007) 1CrossRefGoogle Scholar
  2. 2.
    P.J. Weathers, R.D. Cheetham, E. Follansbee, T. Teoh; Biotechnol Lett 16 (1994) 1281Google Scholar
  3. 3.
    C.Z. Liu, Y.C. Wang, F. Ouyang, H.C. Ye, and G.F. Li; Biotech. Lett. 19 (1997) 927CrossRefGoogle Scholar
  4. 4.
    P.J. Weathers, DeJesus-Gonzalez, Y.J. Kim, F.F Souret, M.J. Towler; Plant Cell Rep. 23 (2004) 414CrossRefGoogle Scholar
  5. 5.
    P.J. Weathers, D.D. Hemmavanh, D.B. Walcerz, R.D. Cheetham, T.C. Smith; In Vitro Cell Dev Biol Plant 33 (1997) 306CrossRefGoogle Scholar
  6. 6.
    T.C. Smith, P.J. Weathers, R.D. Cheetham; In Vitro Cell Dev Biol Plant 33 (1997) 75CrossRefGoogle Scholar
  7. 7.
    K. Wobbe, X. Zhang, P. Weathers, Radical biology:Flores HE, Lynch JP, Eissenstat D, Eds. Rockville, MD: American Society of Plant Physiologists. (1998) p 432.Google Scholar
  8. 8.
    G. Prakash and A.K. Srivastava; Proc Biochem 40 (2005) 3795.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • N. Patra
    • 1
  • S. Sharma
    • 1
  • A. K. Srivastava
    • 1
  1. 1.Department of Biochemical Engineering & BiotechnologyIndian Institute of TechnologyNew DelhiIndia

Personalised recommendations