High Performance Thin Layer Chromatographic Method for the Estimation of Cholesterol in Edible Oils

  • S. Medhe
  • R. Rani
  • K. R. Raj
  • M. M. Srivastava


Cholesterol was detected in six edible market available oil brands using high performance thin layer chromatography. Standard conditions have been optimized based on simulation in Rf values under experimental conditions of polarity of mobile phase and saturation time of solvent chamber. The peanut oil contains highest (0.71 %) while coconut oil contains lowest (0.15 %) cholesterol level. Among the oils studied, no oil was found cholesterol free.


High Performance Thin Layer Chromatography Standard Cholesterol WinCATS Software High Performance Thin Layer Chromatography Plate Solvent Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    H.P. Raven and B.G. Johnson; Biology. pp. 1231. Mc Graw-Hill, New York. 1999.Google Scholar
  2. 2.
    E.J. Behrman and G. Venkat; J. Chem. Edu. 82 (2005) 1791–1793. CrossRefGoogle Scholar
  3. 3.
    K.M. Anderson, W.P. Castelli and D. Levy; J. Am. Med. Assoc. 257 (1987) 2176–2180. CrossRefGoogle Scholar
  4. 4.
    V.K.S. Shukla, P.C. Dutta and W.E. Artz; J. Am. Oil Chem. Soc. 79 (2002) 965–969. CrossRefGoogle Scholar
  5. 5.
    E.O. Aluyor, C.E. Ozigagu, O.I. Oboh and P. Aluyor; Scientific Res. Essay. 4 (2009) 191–197.Google Scholar
  6. 6.
    A. Cert, W. Moreda and C. Perez; J. Chromat. A. 881 (2000) 131–148. CrossRefGoogle Scholar
  7. 7.
    G. Bazylak, H. Brózik and W. Sabanty; Polish J. Environ. Stud. 9 (2000) 113–123.Google Scholar
  8. 8.
  9. 9.
    P.K. Tiwari and P. Sathe; Adv. Biosci. Biotechnol. 1 (2010) 131–135.CrossRefGoogle Scholar
  10. 10.
    E.A. Abourashed and J.S. Mossa; J. Pharm. Biomed. Anal. 36 (2004) 617–620. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • S. Medhe
    • 1
  • R. Rani
    • 1
  • K. R. Raj
    • 1
  • M. M. Srivastava
    • 1
  1. 1.Department of ChemistryDayalbagh Educational InstituteDayalbagh, AgraIndia

Personalised recommendations