Identification of Flavonoids in The Bark of Alstonia Scholaris by High Performance Liquid Chromatography- Electrospray Mass Spectrometry
Alstonia scholaris is traditionally known for its medicinal properties. It contains many phenolic compounds that may have potential as antioxidant. High performance liquid chromatography coupled with electrospray mass spectrometry (LC-ESI/MS) was used for the identification of flavonoids in the bark of Alstonia scholaris. Mobile phase used in the chromatographic separation was 2mM ammonium acetate buffer (solvent-A, pH-2.5 with acetic acid) and acetonitrile (solvent-B) with gradient programming. The peaks were identified by the comparison of retention time, UV-vis spectroscopic and mass spectrometric data with authentic standards and/or literature data. The identified flavonoids included three anthocyanins (cyanidin 3-O-galactoside, cyanidin 3-O-glucoside and malvidin 3-O-glucoside) and three flavonol glycoside (quercetin 3-O-galactoside, quercetin 3-O-glucoside and kaempferol 3-O-glucoside).
KeywordsAuthentic Standard High Antioxidant Activity Bark Extract Electrospray Mass Spectrometry Flavonol Glycoside
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