Antibiotic Resistance Profiles in Relation to Virulence Factors and Phylogenetic Groups of Uropathogenic Escherichia coli Isolated from Dogs and Cats

  • C. Tramuta
  • D. Nucera
  • P. Robino
  • S. Salvarani
  • P. Nebbia
Chapter

Abstract

In this study, we assessed the relationship between antibiotic-resistance profiles, virulence genotype, and phylogenetic group within a collection of Escherichia coli obtained from dogs and cats with urinary tract infection (UTI). Forty uropathogenic E. coli (UPEC) strains isolated from dogs (n=30) and cats (n=10), formerly analyzed for virulence factors (VFs) and phylogenetic group, were tested to detect antibiotic resistance for gentamicin (GM), trimethoprim–sulfamethoxazole (SXT), nitrofurantoin (NT), enrofloxacin (ENO), cephalothin (CF), cephoperazone (CFP), cefovecin (CVN), piperacillin (PIP), and amoxicillin–clavulanic acid (AMC). A large number of isolates were resistant to cephalosporins, especially to third-generation drugs, while the lowest level of resistance was found to SXT. No statistically significant results were obtained (P>0.05). Concerning antimicrobial resistance associated with VFs, only gene iutA showed an association trend with multidrug resistance (MDR; P=0.055). Resistant strains were distributed in all phylogenetic groups (57%, B2; 43%, non-B2), whereas E. coli isolates susceptible to all antibiotics tested were associated with groups B2 (90%) and D (10%).

Keywords

Antimicrobial resistance Cats Dogs Escherichia coli Phylogroup Urine Urine virulence factors 

Abbreviations

MDR

Multidrug-resistance

PCR

Polymerase chain reaction

UPEC

Uropathogenic E. coli

UTI

Urinary tract infection

VFs

Virulence factors

Notes

Acknowledgments

The study was supported by a grant from University research MURST ex-60%.

References

  1. Féria CP, Correia JD, Gonçalves J, Machado J (2000) Detection of virulence factors in uropathogenic Escherichia coli isolated from humans, dogs and cats in Portugal. Adv Exp Med Biol 485:305–308PubMedCrossRefGoogle Scholar
  2. Johnson JR, Kuskowski MA, Owens K, Gajewski A, Winokur PL (2003) Phylogenetic origin and virulence genotype in relation to resistance to fluoroquinolones and/or extended-spectrum cephalosporins and cephamycins among Escherichia coli isolates from animals and humans. J Infect Dis 188:759–768PubMedCrossRefGoogle Scholar
  3. Johnson JR, Kuskowski MA, Gajewski A, Sahm DF, Karlowsky JA (2004) Virulence characteristics and phylogenetic background of multidrug-resistant and antimicrobial-susceptible clinical isolates of Escherichia coli from across the United States, 2000–2001. J Infect Dis 190:1739–1744PubMedCrossRefGoogle Scholar
  4. National Committee for Clinical Laboratory Standards (2001) Development of in vitro susceptibility testing criteria and quality control parameters. Approved guideline M23-A2. National Committee for Clinical Laboratory Standards, Wayne, PAGoogle Scholar
  5. Rijavec M, Starcic Erjavec M, Ambrozic Avgustin J, Reissbrodt R, Fruth A, Krizan-Hergouth V, Zgur Bertok D (2006) High prevalence of multidrug resistance and random distribution of mobile genetic elements among uropathogenic Escherichia coli (UPEC) of the four major phylogenetic groups. Curr Microbiol 53:L158–L162Google Scholar
  6. Tramuta C, Nucera D, Robino P, Salvarani S, Nebbia P (2011) Virulence factors and genetic variability of uropathogenic Escherichia coli isolated from dogs and cats in Italy. J Vet Med 12(1):49–55Google Scholar
  7. Wilson RA, Keefe TJ, Davis MA, Browning MT, Ondrusek K (1988) Strains of Escherichia coli associated with urogenital disease in dogs and cats. Am J Vet Res 49:743–746PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • C. Tramuta
    • 1
  • D. Nucera
    • 2
  • P. Robino
    • 1
  • S. Salvarani
    • 1
  • P. Nebbia
    • 1
  1. 1.Dipartimento di Produzioni Animali, Epidemiologia ed EcologiaUniversità di TorinoTorinoItaly
  2. 2.Dipartimento di Patologia AnimaleUniversità di TorinoTorinoItaly

Personalised recommendations