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Staphylococcus aureus: Application of a Rapid Test for Molecular Typing of Strains Isolated from Bovine Mastitis

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Veterinary Science

Abstract

Staphylococcus aureus is the most important etiologic agent of contagious mastitis able to produce a number of virulence factors that may play important roles in the development of this disease. Recently, a rapid technique based on the amplification of the 16S–23S rRNA intergenic space (RS-PCR) was developed for S. aureus genotyping The aim of the present study was to characterize certain strains collected from North Italy herds to verify the presence of a specific genotype associated in Switzerland with severe clinical expression in the herd. In the 30 strains analyzed, our preliminary results showed the presence of four isolates similar to Switzerland genotype B and the possibility to link an electrophoretic profile to a specific pattern of virulence.

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Correspondence to P. Cremonesi .

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© 2012 Springer-Verlag Berlin Heidelberg

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Cremonesi, P., Benedetti, V., Pisoni, G., Moroni, P., Luini, M., Castiglioni, B. (2012). Staphylococcus aureus: Application of a Rapid Test for Molecular Typing of Strains Isolated from Bovine Mastitis. In: Pugliese, A., Gaiti, A., Boiti, C. (eds) Veterinary Science. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-23271-8_7

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