NOS Immunoreactivity in the Reticular Groove of Lamb

  • M. Mazzoni
  • G. Petrosino
  • P. Clavenzani
  • F. Piccinno
  • V. Grasso
  • M. Serra
  • F. Rambaldi
  • G. Lalatta-Costerbosa
Chapter

Abstract

Evidence suggests that the reticular groove (RG) may have an important role as the coordination center of reticulo-ruminal motility. The aim of this study was to investigate the presence and distribution of nitric oxide synthase (NOS) neurons and their phenotype in the RG of suckling lamb. Using double immunohistochemistry, many NOS neurons were found along the entire length of the RG and were more abundant in the floor than in the lips. They showed irregular profiles and often colocalized with galanin and dopamine β-hydroxylase but rarely with substance P and tyrosine hydroxylase. We never observed NOS neurons expressing neuropeptides normally found in intrinsic primary sensory neurons, such as calcitonin gene-related peptide, calbindin, IB4, and neurofilament 200kDa. When compared to other districts, the enteric plexuses of the RG showed some peculiar aspects, such as the lack of both neurons in the submucosal plexus and typical sensory neurons.

Keywords

Immunohistochemistry Lamb Nitric oxide synthase Reticular groove 

References

  1. Barahona MV, Sánchez-Fortún S, San Andrés MD, Ballesteros E, Contreras J, San Andrés M (1998) Involvement of the L-arginine/nitric oxide neural pathway in non-adrenergic, non-cholinergic relaxation of the bovine oesophageal groove. J Auton Pharmacol 18(2):65–73PubMedCrossRefGoogle Scholar
  2. Denac M, Kümin G, Scharrer E (1991) Effect of noradrenaline on smooth muscle strips from the reticular groove of adult cattle. Zentralbl Veterinarmed A 38(5):383–388PubMedGoogle Scholar
  3. Gregory PC (1982) Forestomach motility in the chronically vagotomized sheep. J Physiol 328:431–437PubMedGoogle Scholar
  4. Gross KJ, Pothoulakis C (2007) Role of neuropeptides in inflammatory bowel disease. Inflamm Bowel Dis 13(7):918–932PubMedCrossRefGoogle Scholar
  5. Lalatta-Costerbosa G, Mazzoni M, Clavenzani P, Di Guardo G, Mazzuoli G, Marruchella G, De Grossi L, Agrimi U, Chiocchetti R (2007) Nitric oxide synthase immunoreactivity and NADPH-d histochemistry in the enteric nervous system of Sarda breed sheep with different PrP genotypes in whole-mount and cryostat preparations. J Histochem Cytochem 55(4):387–401PubMedCrossRefGoogle Scholar
  6. Mazzoni M, Clavenzani P, Minieri L, Russo D, Chiocchetti R, Lalatta-Costerbosa G (2010) Extrinsic afferents supplying the ovine duodenum and ileum. Res Vet Sci 88(3):361–368PubMedCrossRefGoogle Scholar
  7. Pfannkuche H, Schellhorn C, Schemann M, Gäbel G (2003) Reticular groove and reticulum are innervated by myenteric neurons with different neurochemical codes. Anat Rec A 274A:917–922CrossRefGoogle Scholar
  8. Teixeira AF, Wedel T, Krammer HJ, Kühnel W (1998) Structural differences of the enteric nervous system in the cattle forestomach revealed by whole mount immunohistochemistry. Ann Anat 180(5):393–400PubMedCrossRefGoogle Scholar
  9. Vassileva P, Stoyanov I, Loukanov Y (1978) Neurotransmitted responses of smooth-muscle strips of complex sheep stomach after electrical field stimulation. Acta Physiol Pharmacol Bulg 4(1):11–18PubMedGoogle Scholar
  10. Wong MH, McLeay LM (1988) In vitro spontaneous motility of gastric smooth muscles of the sheep. Q J Exp Physiol 73(4):521–531PubMedGoogle Scholar
  11. Yamamoto Y, Atoji Y, Suzuki Y (1995) Morphological study of the submucosal and mucosal plexuses of the sheep forestomach. Ann Anat 177(5):405–412PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • M. Mazzoni
    • 1
  • G. Petrosino
    • 2
  • P. Clavenzani
    • 1
  • F. Piccinno
    • 1
  • V. Grasso
    • 3
  • M. Serra
    • 3
  • F. Rambaldi
    • 1
  • G. Lalatta-Costerbosa
    • 1
  1. 1.DIMORFIPAUniversity of BolognaBolognaItaly
  2. 2.Department of Animal, Plant and Environmental ScienceUniversity of MoliseCampobassoItaly
  3. 3.Department of Clinical MedicineUniversity of BolognaBolgnaItaly

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