NOS Immunoreactivity in the Reticular Groove of Lamb
Evidence suggests that the reticular groove (RG) may have an important role as the coordination center of reticulo-ruminal motility. The aim of this study was to investigate the presence and distribution of nitric oxide synthase (NOS) neurons and their phenotype in the RG of suckling lamb. Using double immunohistochemistry, many NOS neurons were found along the entire length of the RG and were more abundant in the floor than in the lips. They showed irregular profiles and often colocalized with galanin and dopamine β-hydroxylase but rarely with substance P and tyrosine hydroxylase. We never observed NOS neurons expressing neuropeptides normally found in intrinsic primary sensory neurons, such as calcitonin gene-related peptide, calbindin, IB4, and neurofilament 200kDa. When compared to other districts, the enteric plexuses of the RG showed some peculiar aspects, such as the lack of both neurons in the submucosal plexus and typical sensory neurons.
KeywordsImmunohistochemistry Lamb Nitric oxide synthase Reticular groove
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