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Metabolic Engineering of Cyanobacteria for Direct Conversion of CO2 to Hydrocarbon Biofuels

  • Christer JanssonEmail author
Part of the Progress in Botany book series (BOTANY, volume 73)

Abstract

Cyanobacteria are oxygenic photosynthesizers like plant and algae and hence can capture CO2 via the Calvin cycle and convert it to a suite of organic compounds. They are Gram-negative bacteria and are well suited for synthetic biology and metabolic engineering approaches for the phototrophic production of various desirable biomolecules, including ethanol, butanol, biodiesel, and hydrocarbon biofuels. Phototrophic biosynthesis of high-density liquid biofuels in cyanobacteria would serve as a good complement to the microbial production of biodiesel and hydrocarbons in heterotrophic bacteria such as Escherichia coli. Two groups of hydrocarbon biofuels that are being considered in microbial production systems are alkanes and isoprenoids. Alkanes of defined chain lengths can be used as drop-in fuel similar to gasoline and jet fuel. Many cyanobacteria synthesize alkanes, albeit in minute quantities. Optimizing the expression of the alkane biosynthesis genes and enhancing the carbon flux through the fatty acid and alkane biosynthesis pathways should lead to the accumulation and/or secretion of notable amounts of alkanes. It also becomes important to understand how to control the chain lengths of the produced alkane molecules. Isoprenoids, e.g., the monoterpene pinene and the sesquiterpene farnesene, are considered precursors for future biodiesel or next-generation jet fuel. Cyanobacteria produce carotenoids and extending the carotenoid biosynthetic pathways by the introduction of constructs for appropriate terpene synthases should allow the biosynthesis of selected mono- and sesquiterpenes.

Keywords

Fatty Acid Synthesis Algal Biofuel Raceway Pond Synechococcus Elongatus Geranyl Pyrophosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported in part by U. S. Department of Energy Contract DE-AC02-05CH11231 with Lawrence Berkeley National Laboratory. Funding from the DOE-LDRD grant CyanoAlkanes is acknowledged.

References

  1. Agger SA, Lopez-Gallego F, Hoye TR, Schmidt-Dannert C (2008) Identification of sesquiterpene synthases from Nostoc punctiforme PCC 73102 and Nostoc sp strain PCC 7120. J Bacteriol 190:6084–6096PubMedCrossRefGoogle Scholar
  2. Atsumi S, Higashide W, Liao JC (2009) Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde. Nat Biotechnol 27:1177–1180PubMedCrossRefGoogle Scholar
  3. Beller HR, Goh EB, Keasling JD (2010) Genes involved in long-chain alkene biosynthesis in Micrococcus luteus. Appl Environ Microb 76:1212–1223CrossRefGoogle Scholar
  4. Bhadauriya P, Gupta R, Singh S, Bisen PS (2008) n-Alkanes variability in the diazotrophic cyanobacterium Anabaena cylindrica in response to NaCl stress. World J Microb Biot 24:139–141CrossRefGoogle Scholar
  5. Bonaventure G, Salas JJ, Pollard MR, Ohlrogge JB (2003) Disruption of the FATB gene in Arabidopsis demonstrates an essential role of saturated fatty acids in plant growth. Plant Cell 15:1020–1033PubMedCrossRefGoogle Scholar
  6. Campbell JW, Cronan JE (2001) Bacterial fatty acid biosynthesis: targets for antibacterial drug discovery. Annu Rev Microbiol 55:305–332PubMedCrossRefGoogle Scholar
  7. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306PubMedCrossRefGoogle Scholar
  8. Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:126–131PubMedCrossRefGoogle Scholar
  9. Cho H, Cronan JE (1994) Protease-I of Escherichia coli functions as a thioesterase in-vivo. J Bacteriol 176:1793–1795PubMedGoogle Scholar
  10. Clarens AF, Resurreccion EP, White MA, Colosi LM (2010) Environmental life cycle comparison of algae to other bioenergy feedstocks. Environ Sci Technol 44:1813–1819PubMedCrossRefGoogle Scholar
  11. Costa JAV, de Morais MG (2011) The role of biochemical engineering in the production of biofuels from microalgae. Bioresour Technol 102(1):2–9PubMedCrossRefGoogle Scholar
  12. Cronan JE (2003) Bacterial membrane lipids: where do we stand? Annu Rev Microbiol 57:203–224PubMedCrossRefGoogle Scholar
  13. Dembitsky VM, Dor I, Shkrob I, Aki M (2001) Branched alkanes and other apolar compounds produced by the cyanobacterium Microcoleus vaginatus from the Negev Desert. Russ J Bioorg Chem 27:110–119CrossRefGoogle Scholar
  14. Deng MD, Coleman JR (1999) Ethanol synthesis by genetic engineering in cyanobacteria. Appl Environ Microb 65:523–528Google Scholar
  15. Fortman JL, Chhabra S, Mukhopadhyay A, Chou H, Lee TS, Steen E, Keasling JD (2008) Biofuel alternatives to ethanol: pumping the microbial well. Trends Biotechnol 26:375–381PubMedCrossRefGoogle Scholar
  16. Gressel J (2008) Transgenics are imperative for biofuel crops. Plant Sci 174:246–263CrossRefGoogle Scholar
  17. Hagio M, Gombos Z, Varkonyi Z, Masamoto K, Sato N, Tsuzuki M, Wada H (2000) Direct evidence for requirement of phosphatidylglycerol in photosystem II of photosynthesis. Plant Physiol 124:795–804PubMedCrossRefGoogle Scholar
  18. Hillen LW, Pollard G, Wake LV, White N (1982) Hydrocracking of the oils of Botryococcus braunii to transport fuels. Biotechnol Bioeng 24:193–205PubMedCrossRefGoogle Scholar
  19. Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621–639PubMedCrossRefGoogle Scholar
  20. Jansson C, Northen T (2010) Calcifying cyanobacteria – the potential of biomineralization for carbon capture and storage. Curr Opin Biotech 21:365–371PubMedCrossRefGoogle Scholar
  21. Jha JK, Maiti MK, Bhattacharjee A, Basu A, Sen PC, Sen SK (2006) Cloning and functional expression of an acyl-ACP thioesterase FatB type from Diploknema (Madhuca) butyracea seeds in Escherichia coli. Plant Physiol Bioch 44:645–655CrossRefGoogle Scholar
  22. Jones A, Davies HM, Voelker TA (1995) Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Plant Cell 7:359–371PubMedGoogle Scholar
  23. Kaczmarzyk D, Fulda M (2010) Fatty acid activation in cyanobacteria mediated by acyl–acyl carrier protein synthetase enables fatty acid recycling. Plant Physiol 152:1598–1610PubMedCrossRefGoogle Scholar
  24. Kalscheuer R, Stolting T, Steinbuchel A (2006) Microdiesel: Escherichia coli engineered for fuel production. Microbiology 152:2529–2536PubMedCrossRefGoogle Scholar
  25. Keasling JD, Chou H (2008) Metabolic engineering delivers next-generation biofuels. Nat Biotechnol 26:298–299PubMedCrossRefGoogle Scholar
  26. Khosla C (2008) Microbial synthesis of biodiesel Book Microbial synthesis of biodiesel. Stanford University, StanfordGoogle Scholar
  27. Kirby J, Keasling JD (2008) Metabolic engineering of microorganisms for isoprenoid production. Nat Prod Rep 25:656–661PubMedCrossRefGoogle Scholar
  28. Ladygina N, Dedyukhina EG, Vainshtein MB (2006) A review on microbial synthesis of hydrocarbons. Process Biochem 41:1001–1014CrossRefGoogle Scholar
  29. Lennen RM, Braden DJ, West RM, Dumesic JA, Pfleger BF (2010) A process for microbial hydrocarbon synthesis: overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes. Biotechnol Bioeng 106:193–202PubMedCrossRefGoogle Scholar
  30. Li W, Liu XX, Wang W, Sun H, Hu YM, Lei H, Liu GH, Gao YY (2008) Effects of antisense RNA targeting of ODC and AdoMetDC on the synthesis of polyamine synthesis and cell growth in prostate cancer cells using a prostatic androgen-dependent promoter in adenovirus. Prostate 68:1354–1361PubMedCrossRefGoogle Scholar
  31. Lindberg P, Park S, Melis A (2010) Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab Eng 12:70–79PubMedCrossRefGoogle Scholar
  32. Liu XY, Curtiss R (2009) Nickel-inducible lysis system in Synechocystis sp PCC 6803. Proc Natl Acad Sci USA 106:21550–21554PubMedCrossRefGoogle Scholar
  33. Lu X (2010) A perspective: photosynthetic production of fatty acid-based biofuels in genetically engineered cyanobacteria. Biotechnol Adv 28(6):742–746PubMedCrossRefGoogle Scholar
  34. Lu XF, Vora H, Khosla C (2008) Overproduction of free fatty acids in E. coli: implications for biodiesel production. Metab Eng 10:333–339PubMedCrossRefGoogle Scholar
  35. Ono E, Cuello JL (2007) Carbon dioxide mitigation using thermophilic cyanobacteria. Biosyst Eng 96:129–134CrossRefGoogle Scholar
  36. Packer M (2009) Algal capture of carbon dioxide; biomass generation as a tool for greenhouse gas mitigation with reference to New Zealand energy strategy and policy. Energy Policy 37:3428–3437CrossRefGoogle Scholar
  37. Radakovits R, Jinkerson RE, Darzins A, Posewitz MC (2010) Genetic engineering of algae for enhanced biofuel production. Eukaryot Cell 9:486–501PubMedCrossRefGoogle Scholar
  38. Rude MA, Schirmer A (2009) New microbial fuels: a biotech perspective. Curr Opin Microbiol 12:274–281PubMedCrossRefGoogle Scholar
  39. Schirmer A, Rude MA, Li XZ, Popova E, del Cardayre SB (2010) Microbial biosynthesis of alkanes. Science 329:559–562PubMedCrossRefGoogle Scholar
  40. Scott SA, Davey MP, Dennis JS, Horst I, Howe CJ, Lea-Smith DJ, Smith AG (2010) Biodiesel from algae: challenges and prospects. Curr Opin Biotechnol 21:277–286PubMedCrossRefGoogle Scholar
  41. Sheehan J (2009) Engineering direct conversion of CO2 to biofuel. Nat Biotechnol 27:1128–1129PubMedCrossRefGoogle Scholar
  42. Steen EJ, Kang YS, Bokinsky G, Hu ZH, Schirmer A, McClure A, del Cardayre SB, Keasling JD (2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature 463:559–562PubMedCrossRefGoogle Scholar
  43. Thelen JJ, Ohlrogge JB (2002) Metabolic engineering of fatty acid biosynthesis in plants. Metab Eng 4:12–21PubMedCrossRefGoogle Scholar
  44. Voelker TA, Davies HM (1994) Alteration of the specificity and regulation of fatty-acid synthesis of Escherichia coli by expression of a plant medium-chain acyl–acyl carrier protein thioesterase. J Bacteriol 176:7320–7327PubMedGoogle Scholar
  45. Voelker T, Kinney AT (2001) Variations in the biosynthesis of seed-storage lipids. Annu Rev Plant Physiol 52:335–361CrossRefGoogle Scholar
  46. Walsh K, Jones GJ, Dunstan RH (1998) Effect of high irradiance and iron on volatile odour compounds in the cyanobacterium Microcystis aeruginosa. Phytochemistry 49:1227–1239PubMedCrossRefGoogle Scholar
  47. Warui DM, Li N, Nørgaard H, Krebs C, Bollinger JM Jr, Booker SJ (2011) Detection of formate, rather than carbon monoxide, as the stoichiometric coproduct in conversion of fatty aldehydes to alkanes by a cyanobacterial aldehyde decarbonylase. J Am Chem Soc 133:3316–3319PubMedCrossRefGoogle Scholar
  48. Yoo JH, Cheng OH, Gerber GE (2001) Determination of the native form of FadD, the Escherichia coli fatty acyl-CoA synthetase, and characterization of limited proteolysis by outer membrane protease OmpT. Biochem J 360:699–706PubMedCrossRefGoogle Scholar
  49. Yuan L, Voelker TA, Hawkins DJ (1995) Modification of the substrate-specificity of an acyl–acyl carrier protein thioesterase by protein engineering. Proc Natl Acad Sci USA 92:10639–10643PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Lawrence Berkeley National LaboratoryBerkeleyUSA

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