Abstract
The behaviour of extracelullar hydrolytic enzyme activities in a continuous-supply vermicomposting system has never been studied previously. The aim of this study was to assess under such system the extracellular enzyme activities glucosidase, urease, acid phosphatase, and protease, which were extracted following the pyrophosphate-extraction method at pH 7.1. Vermicomposting was carried out using a medium-sized rectangular vermireactor continually supplied with damaged tomatoes (10 kg week−1) during a 5-month period on a layer of mature sheep manure. The earthworms were then removed, and the vermicomposted organic material was matured over a 2-month period. Total earthworm biomass reached its greatest value after 3 months, coinciding with peak levels for pyrophosphate-extractable carbon content and extracellular urease, acid phosphatase and protease activities. Extracellular β-glucosidase activity peaked in the 4th month, when microbial activity was also at its greatest level. At the end of the vermicomposting period, phosphatase and protease activity decreased to levels similar to those recorded at the beginning of the vermicomposting process. By contrast, β-glucosidase and urease activity values were grater than those recorded at the start of the vermicomposting process. Humic-enzyme complexes generated during the vermicomposting period were unable to resist denaturation, inactivation, and degradation caused by the air-drying during the maturation phase. Although the mature vermicompost obtained showed higher content in humus-enzyme complexes than the initial mature sheep manure, the assayed continuous-supply vermicomposting system was unefficient for enhancing the formation of stabilized humus-enzymes complexes.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Benítez E, Nogales R, Elvira C, Masciandaro G, Ceccanti B (1999) Enzymes activities as indicators of the stabilization of sewage sludges composting by Eisenia andrei. Bioresour Technol 67:297–303
Benítez E, Nogales R, Masciandaro G, Ceccanti B (2000) Isolation by IEF of humic–urease complexes from earthworms processed sewage sludges. Biol Fertil Soils 31:489–493
Benítez E, Melgar R, Nogales R (2004) Estimating soil resilience to toxic waste by measuring enzyme activities. Soil Biol Biochem 36:1615–1621
Benítez E, Sainz H, Nogales R (2005) Hydrolytic enzyme activities of extracted humic substances during the vermicomposting of a lignocellulosic olive waste. Bioresour Technol 96:785–790
Burns RG (1982) Enzyme activity in soil: location and a possible role in microbial ecology. Soil Biol Biochem 14:423–427
Dick RP (1994) Soil enzyme activities as indicators of soil quality. In: Doran JW, Coleman DC, Bezdicek DF, Stewart BA (eds) Defining soil quality for a sustainable environment. American Society of Agronomy-Soil Science Society of America, Special Publication No. 35, Madison,WI, pp 107–124
Edwards CA (1995) Historical overview of vermicomposting. BioCycle 36:56–58
Elvira C, Sampedro L, Benítez E, Nogales R (1998) Vermicomposting of sludges from paper mill and dairy industries with Eisenia andrei: a pilot scale study. Bioresour Technol 6:205–211
García C, Henández T, Costa F, Ceccanti C, Ganni A (1993) Hydrolases in the organic matter fractions of sewage sludge: changes with composting. Bioresour Technol 45:47–52
García C, Hernández T, Costa F (1997) Potencial use of dehydrogenase activity as an index of microbial activity in degraded soils. Commun Soil Sci Plan28:123–134
Kandeler E, Gerber H (1988) Short-term assay of soil urease activity using colorimetric determination of ammonium. Biol Fertil Soils 6:68–72
MAPA (1986) Métodos Oficiales de Análisis. Vol III Plantas, Productos Orgánicos Fertilizantes, Suelos, Agua, Productos Fitosanitarios y Fertilizantes Inorgánicos. Publicaciones del Ministerio de Agricultura, Pesca y Alimentación, Madrid, Spain
McCarty GW, Shogren DR, Bremner JM (1992) Regulation of urease production in soil by microbial assimilation of nitrogen. Biol Fertil Soils 12:261–264
Melgar R, Benítez E, Nogales R (2009) Bioconversion of wastes from olive oil industries using the epigeic earthworm Eisenia Andrei. J Environ Sci Health–Part B 44:488–495
Nannipieri P, Ceccanti B, Cervelli S, Matarese E (1980) Extraction of phosphatase, urease, protease, organic carbon and nitrogen from soil. Soil Sci Soc Am J 44:1011–1016
Nannipieri P, Sequi P, Fusi P (1996) Humus and Enzyme Activity. In: Piccolo A (ed) Humic substances in terrestrial ecosystems. Elsevier Science, Amsterdam, The Netherlands, pp 293–328
Nogales R, Cifuentes C, Benítez E (2005) Vermicomposting of winery wastes: A laboratory study. J Environ Sci Health–Part B 49:659–673
Nogales R, Domínguez J, Mato S (2008) Vermicompostaje. In: Moreno J, Moral R (eds) Compostaje. Editorial Mundi-Prensa, Madrid, Spain pp 187–2008
Parthasarathi K, Ranganathan LS (1999) Longevity of microbial and enzyme activity and their influence on NPK content in pressmud vermicasts. Eur J Soil Biol 35:107–113
Parthasarathi K, Ranganathan LS (2000) Aging effect on enzyme activities in pressmud vermicasts of Lampito mauritii (Kinberg) and Eudrilus eugeniae (Kinberg). Biol Fertil Soils 30:347–350
Pascual JA, Moreno JL, Hernández T, García C (2002) Persistence of immobilised and total urease and phosphatase activities in a soil amended with organic wastes. Bioresour Technol 82:73–78
Romero E, Benítez E, Nogales R (2005) Suitability of wastes from olive-oil industry for initial reclamation of a Pb/Zn mine tailing. Water Air Soil Pollut 165:153–165
Sims JR, Haby VA (1971) Simplified colorimetric determination of soil organic matter. Soil Sci 112:137–141
Tabatabai MA, Bremner JM (1969) Use of p-nitrophenylphosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307
Xu JM, Tang C, Chen ZL (2006) The role of plant residues in pH change of acid soils differing in initial pH. Soil Biol Biochem 38:709–719
Acknowledgements
This study supported by Junta de Andalucía (Project P05-AGR-00408). M. Fernández-Gómez thanks the Science and Innovation Ministry for his FPU doctoral grant (AP2006-03452). Finally, we would also like to thank Michael O’Shea for proofreading.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Fernández-Gómez, M.J., Romero, E., Cifuentes, C., Nogales, R. (2011). Hydrolases Activities of Extracted Humic Substances During Vermicomposting of Damaged Tomatoes Wastes Using a Continuous-Supplying System. In: Trasar-Cepeda, C., Hernández, T., García, C., Rad, C., González-Carcedo, S. (eds) Soil Enzymology in the Recycling of Organic Wastes and Environmental Restoration. Environmental Science and Engineering(). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-21162-1_22
Download citation
DOI: https://doi.org/10.1007/978-3-642-21162-1_22
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-21161-4
Online ISBN: 978-3-642-21162-1
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)