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Cytogenetics as a Tool for an Exploration of A. sturio Status Within Sturgeons

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Biology and Conservation of the European SturgeonAcipenser sturioL. 1758

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Abbreviations

Cytochrome b (cyt b):

A protein component of respiratory chain, involved in electron transport and the production of ATP.

Fluorescent in situ hybridization (FISH):

Cytogenetic technique used to detect and localize the presence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that only bind to the chromosome parts with high sequence similarity.

Haplotype:

An haplotype is a combination of DNA sequences on one chromosome which tend to be inherited together.

HindIII and PstI satellite DNA:

Two satellite DNA families (respectively identified by restriction enzymes of Haemophilus indicus and Providencia stuartii) frequently studied in sturgeons.

Satellite DNA:

Short and highly repeated DNA sequences. Most satellite DNA is localized to the telomeric or the centromeric region of the chromosomes. The nucleotide sequence of the repeats is fairly well conserved among species.

sim-FISH:

Simultaneous detection of the two gene families by different staining of two different gene probes. The simultaneous visualization of the two colours is obtained with an epifluorescence microscope equipped with special band filters.

Telomeric sequence:

A region of repetitive DNA sequences located at the end of a chromosome, which protects the end of the chromosome from being cut at the end of linear DNA replication.

28S, 16S, 5S rDNA:

Regions of DNA respectively codifying for 28S, 16S and 5S ribosomal RNAs. They are composed of tandem repeats of sequences.

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Fontana, F. (2011). Cytogenetics as a Tool for an Exploration of A. sturio Status Within Sturgeons. In: Williot, P., Rochard, E., Desse-Berset, N., Kirschbaum, F., Gessner, J. (eds) Biology and Conservation of the European SturgeonAcipenser sturioL. 1758. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20611-5_2

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