Abstract
The beneficial role of lung protective ventilation in patients with Acute Lung Injury (ALI) or Acute Respiratory Distress Syndrome (ARDS) has become evident. However, knowledge about the effects of mechanical ventilation at the alveolar level is still limited. In vivo microscopy could help to understand alveolar mechanics. We tested a relatively new, less invasive method of in vivo microscopy not influencing the mechanical behaviour of alveoli and giving depth information about the tissue. The aim of this first observation was to demonstrate feasibility of a fibered confocal laser scanning microscopy (CLSM) used endoscopically and through a thoracic window in a rabbit model. Both endoscopic application and use through a thoracic window provided high-resolution images of alveolar structure. Within experimental ALI, relevant heterogeneity with collapsed adjacent to expanded regions was observable. Quantification measurements may help to further evaluate alveolar mechanics.
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Bickenbach, J., Czaplik, M., Rossaint, R. (2011). In Vivo Microscopy and Analysis of Regional Ventilation in a Porcine Model of Acute Lung Injury. In: Klaas, M., Koch, E., Schröder, W. (eds) Fundamental Medical and Engineering Investigations on Protective Artificial Respiration. Notes on Numerical Fluid Mechanics and Multidisciplinary Design, vol 116. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20326-8_6
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DOI: https://doi.org/10.1007/978-3-642-20326-8_6
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