Gene Therapy Used for Adipose Stem Cell Engineering

Chapter

Abstract

The fundamental principal of gene therapy is the transfer of genetic material into individuals for therapeutic purposes by altering cellular function or structure at the molecular level. The genetic alteration ultimately leads to the production of a therapeutic protein that is secreted into the surrounding tissue milieu, is expressed on the cell surface or acts as a signaling molecule to influence cell or tissue behavior. Genetic engineering and the use of adult stem cells may hold the key to future development of tissue-engineered constructs. The identification or perhaps deletion of specific genetic sequences might be able to identify and modify genes critical to tissue development. The overall safety of the various gene delivery systems is also an important consideration. Clearly, many hurdles remain to be addressed before these approaches can be widely applied as a common therapeutic strategy.

Keywords

Stem Cell Gene Therapy Osteogenesis Imperfecta Adult Stem Cell Fanconi Anemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1.  1.
    Bennett JH et al (1991) Adipocytic cells cultured from marrow have osteogenic potential. J Cell Sci 99(pt 1):131–139PubMedGoogle Scholar
  2.  2.
    Bonadio J (2000) Tissue engineering via local gene delivery: update and future prospects for enhancing the technology. Adv Drug Deliv Rev 44(2-3):185–194PubMedCrossRefGoogle Scholar
  3.  3.
    Bonadio J (2002) Genetic approaches to tissue repair. Ann NY Acad Sci 961:58–60PubMedCrossRefGoogle Scholar
  4.  4.
    Bonadio J et al (1999) Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration. Nat Med 5(7):753–759PubMedCrossRefGoogle Scholar
  5.  5.
    Bruder SP, Fink DJ, Caplan AI (1994) Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem 56(3):283–294PubMedCrossRefGoogle Scholar
  6.  6.
    Bruder SP, Jaiswal N, Haynesworth SE (1997) Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem 64(2):278–294PubMedCrossRefGoogle Scholar
  7.  7.
    Burton EA, Glorioso JC, Fink DJ (2003) Gene therapy progress and prospects: Parkinson’s disease. Gene Ther 10(20):1721–1727PubMedCrossRefGoogle Scholar
  8.  8.
    Chamberlain JR et al (2004) Gene targeting in stem cells from individuals with osteogenesis imperfecta. Science 303(5661):1198–1201PubMedCrossRefGoogle Scholar
  9.  9.
    Chen HK et al (2005) Combined cord blood stem cells and gene therapy enhances angiogenesis and improves cardiac performance in mouse after acute myocardial infarction. Eur J Clin Invest 35(11):677–686PubMedCrossRefGoogle Scholar
  10. 10.
    Crystal RG (1995) Transfer of genes to humans: early lessons and obstacles to success. Science 270(5235):404–410PubMedCrossRefGoogle Scholar
  11. 11.
    Danos O, Heard JM (1992) Recombinant retroviruses as tools for gene transfer to somatic cells. Bone Marrow Transplant 9(suppl 1):131–138PubMedGoogle Scholar
  12. 12.
    De Ugarte DA et al (2003) Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells Tissues Organs 174(3):101–109PubMedCrossRefGoogle Scholar
  13. 13.
    Evans CH, Robbins PD (1995) Possible orthopaedic applications of gene therapy. J Bone Joint Surg Am 77(7):1103–1114PubMedGoogle Scholar
  14. 14.
    Galotto M et al (1994) Hypertrophic chondrocytes undergo further differentiation to osteoblast-like cells and participate in the initial bone formation in developing chick embryo. J Bone Miner Res 9(8):1239–1249PubMedCrossRefGoogle Scholar
  15. 15.
    Goessler UR, Hormann K, Riedel F (2004) Tissue engineering with chondrocytes and function of the extracellular matrix (review). Int J Mol Med 13(4):505–513PubMedGoogle Scholar
  16. 16.
    Goessler UR, Hormann K, Riedel F (2005) Tissue engineering with adult stem cells in reconstructive surgery (review). Int J Mol Med 15(6):899–905PubMedGoogle Scholar
  17. 17.
    Goessler UR et al (2006) Perspectives of gene therapy in stem cell tissue engineering. Cells Tissues Organs 183(4):169–179PubMedCrossRefGoogle Scholar
  18. 18.
    Goff SP, Lobel LI (1987) Mutants of murine leukemia viruses and retroviral replication. Biochim Biophys Acta 907(2):93–123PubMedGoogle Scholar
  19. 19.
    Hall PA, Watt FM (1989) Stem cells: the generation and maintenance of cellular diversity. Development 106(4):619–633PubMedGoogle Scholar
  20. 20.
    Hammerling GJ, Ganss R (2006) Vascular integration of endothelial progenitors during multistep tumor progression. Cell Cycle 5(5):509–511PubMedCrossRefGoogle Scholar
  21. 21.
    Korbling M, Estrov Z (2003) Adult stem cells for tissue repair–a new therapeutic concept? N Engl J Med 349(6):570–582PubMedCrossRefGoogle Scholar
  22. 22.
    Korbling M, Estrov Z, Champlin R (2003) Adult stem cells and tissue repair. Bone Marrow Transplant 32(suppl 1):S23–S24PubMedCrossRefGoogle Scholar
  23. 23.
    Krisky DM et al (1998) Development of herpes simplex virus replication-defective multigene vectors for com­bination gene therapy applications. Gene Ther 5(11):1517–1530PubMedCrossRefGoogle Scholar
  24. 24.
    Krisky DM et al (1998) Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. Gene Ther 5(12):1593–1603PubMedCrossRefGoogle Scholar
  25. 25.
    Krougliak V, Graham FL (1995) Development of cell lines capable of complementing E1, E4, and protein IX defective adenovirus type 5 mutants. Hum Gene Ther 6(12):1575–1586PubMedCrossRefGoogle Scholar
  26. 26.
    Levitus M, Joenje H, de Winter JP (2006) The Fanconi anemia pathway of genomic maintenance. Cell Oncol 28(1–2):3–29PubMedGoogle Scholar
  27. 27.
    Lucas WT, Youngner JS (1992) The use of hybrid-selected template increases the specificity of the polymerase chain reaction. PCR Meth Appl 2(1):41–44CrossRefGoogle Scholar
  28. 28.
    Marshall E (2000) Improving gene therapy’s tool kit. Science 288(5468):953PubMedCrossRefGoogle Scholar
  29. 29.
    Marshall E (2002) Clinical research. Gene therapy a suspect in leukemia-like disease. Science 298(5591):34–35PubMedCrossRefGoogle Scholar
  30. 30.
    Oligino TJ et al (2000) Vector systems for gene transfer to joints. Clin Orthop Relat Res 379(suppl):S17–S30PubMedCrossRefGoogle Scholar
  31. 31.
    Parker AM, Katz AJ (2006) Adipose-derived stem cells for the regeneration of damaged tissues. Expert Opin Biol Ther 6(6):567–578PubMedCrossRefGoogle Scholar
  32. 32.
    Pittenger MF et al (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284(5411):143–147PubMedCrossRefGoogle Scholar
  33. 33.
    Reyes M et al (2002) Origin of endothelial progenitors in human postnatal bone marrow. J Clin Invest 109(3):337–346PubMedGoogle Scholar
  34. 34.
    Robbins PD, Ghivizzani SC (1998) Viral vectors for gene therapy. Pharmacol Ther 80(1):35–47PubMedCrossRefGoogle Scholar
  35. 35.
    Salyapongse AN, Billiar TR, Edington H (1999) Gene therapy and tissue engineering. Clin Plast Surg 26(4):663–676, xPubMedGoogle Scholar
  36. 36.
    Stock UA, Vacanti JP (2001) Tissue engineering: current state and prospects. Annu Rev Med 52:443–451PubMedCrossRefGoogle Scholar
  37. 37.
    Toma JG et al (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3(9):778–784PubMedCrossRefGoogle Scholar
  38. 38.
    Warren SM et al (2002) New directions in bioabsorbable technology. J Neurosurg 97(4 suppl):481–489PubMedGoogle Scholar
  39. 39.
    Young LS et al (2006) Viral gene therapy strategies: from basic science to clinical application. J Pathol 208(2):299–318PubMedCrossRefGoogle Scholar
  40. 40.
    Zuk PA et al (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7(2):211–228PubMedCrossRefGoogle Scholar
  41. 41.
    Zuk PA et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13(12):4279–4295PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.Department of Otolaryngology, Head and Neck SurgeryUniversitäts-HNO-Klinik MannheimMannheimGermany

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