A Systematic Modeling Approach to Elucidate the Triggering of the Stringent Response in Recombinant E. coli Systems

  • Sónia Carneiro
  • Eugénio C. Ferreira
  • Isabel Rocha
Conference paper
Part of the Advances in Intelligent and Soft Computing book series (AINSC, volume 93)


A hybrid modeling approach, combining a stoichiometric model of the E. coli metabolic network and kinetic-based descriptions for the production of recombinant protein, cell growth and ppGpp synthesis, was applied to describe metabolic bottlenecks associated with recombinant processes. The model represents the triggering of the stringent response upon the deprivation of amino acids caused by the additional drainage of biosynthetic precursors for the production of recombinant proteins. The equation for ppGpp synthesis allows to estimate the accumulation of this molecule above its basal levels once amino acid shortages occur. The capability to predict these stress-responsive events might be crucial in the design of optimal cultivation strategies.


Recombinant Protein Production Flux Balance Analysis Amino Acid Pool Stringent Response Intracellular Amino Acid 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Sónia Carneiro
    • 1
  • Eugénio C. Ferreira
    • 1
  • Isabel Rocha
    • 1
  1. 1.IBB - Institute for Biotechnology and Bioengineering, Centre of Biological EngineeringUniversity of MinhoBragaPortugal

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