Skip to main content
SpringerLink
Log in

Bone Marrow Engraftment Analysis

  • Chapter
  • First Online:
Diagnostic Molecular Pathology in Practice

  • 2281 Accesses

Abstract

A 66-year-old man presented with urinary retention and was having a preoperative evaluation for a transurethral prostatectomy. He was found to have pancytopenia and was referred to a hematologist. The patient had a history of eosinophilic cellulitis three years earlier, which was treated with steroids for one year with complete resolution of the problem. He also had a history of a squamous cell carcinoma of the skin, which was resected. At the time of presentation, he had no bleeding or bruising. He was fatigued but still active and had no recent infections. Medications included tamsulosin for prostatic hypertrophy and supplemental Vitamin D. The patient was a retired purchasing agent with no history of chemical exposure. His parents died of heart disease and one brother with diabetes was still alive. He had two daughters, alive and well.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Haspel RL, Ballen KK (2006) Double cord blood transplants: filling a niche? Stem Cell Rev 2:81–86

    PubMed  Google Scholar 

  2. Van Deerlin VM, Williams E (2007) Assessment of chimerism in the setting of allogeneic hematopoietic cell transplantation. In: Leonard DGB (ed) Molecular pathology clinical practice, 1st edn. Springer, New York, pp 517–531

    Chapter  Google Scholar 

  3. Schichman SA, Lin P, Gilbrech LJ et al (2002) Bone marrow transplant engraftment analysis with loss of an informative allele. J Mol Diagn 4:230–232

    Article  PubMed  PubMed Central  Google Scholar 

  4. Khan F, Agarwal A, Agrawal S (2004) Significance of ­chimerism in hematopoietic stem cell transplantation: new variations on an old theme. Bone Marrow Transplant 34:1–12

    Article  PubMed  CAS  Google Scholar 

  5. McCann SR, Crampe M, Molloy K et al (2005) Hemopoietic chimerism following stem cell transplantation. Transfus Apher Sci 32:55–61

    Article  PubMed  Google Scholar 

  6. Thiede C (2004) Diagnostic chimerism analysis after allogeneic stem cell transplantation: new methods and markers. Am J Pharmacogenomics 4:177–187

    Article  PubMed  CAS  Google Scholar 

  7. Briones J, Urbano-Ispizua A, Lawler M et al (1998) High frequency of donor chimerism after allogeneic transplantation of CD34+?selected peripheral blood cells. Exp Hematol 26:415–420

    PubMed  CAS  Google Scholar 

  8. Bader P, Willasch A, Klingebiel T (2008) Monitoring of post-transplant remission of childhood malignancies: is there a standard? Bone Marrow Transplant 42:S31–S34

    Article  PubMed  Google Scholar 

  9. Antin JH, Childs R, Filipovich AH et al (2001) Establishment of complete and mixed donor chimerism after allogeneic lymphohematopoietic transplantation: recommendations from a workshop at the 2001 Tandem Meetings of the International Bone Marrow Transplant Registry and the American Society of Blood and Marrow Transplantation. Biol Blood Marrow Transplant 7:473–485

    Article  PubMed  CAS  Google Scholar 

  10. Fernandez-Aviles F, Urbano-Ispizua A, Aymerich M et al (2003) Serial quantification of lymphoid and myeloid mixed chimerism using multiplex PCR amplification of short tandem repeat-markers predicts graft rejection and relapse, respectively, after allogeneic transplantation of CD34+ selected cells from peripheral blood. Leukemia 17:613–620

    Article  PubMed  CAS  Google Scholar 

  11. Gluckman E, Rocha V (2004) Cord blood transplant: strategy of alternative donor search. Springer Semin Immu­nopathol 26:143–154

    Article  PubMed  Google Scholar 

  12. Barker JN, Scaradavou A, Stevens CE (2010) Combined effect of total nucleated cell dose and HLA match on transplantation outcome in 1061 cord blood recipients with hematologic malignancies. Blood 115:1843–1849

    Google Scholar 

  13. Gluckman E (2009) Ten years of cord blood transplantation: from bench to bedside. Br J Haematol 147:192–199

    Article  PubMed  CAS  Google Scholar 

  14. Takahashi S (2007) Leukemia: Cord blood for allogeneic stem cell transplantation. Curr Opin Oncol 19:667–672

    Article  Google Scholar 

  15. Barker JN, Weisdorf DJ, Wagner JE (2001) Creation of a double chimera after the transplantation of umbilical-cord blood from two partially matched unrelated donors. N Engl J Med 344:1870–1871

    Article  PubMed  CAS  Google Scholar 

  16. Kogler G, Enczman J, Rocha V et al (2005) High-resolution HLA typing by sequencing for HLA-A, -B, -C, -DR, -DQ in 122 unrelated cord blood/patient pair transplants hardly improves long-term clinical outcome. Bone Marrow Transplant 36:1033–1041

    Article  PubMed  CAS  Google Scholar 

  17. Barker JN, Scaradavou A, Stevens CE (2010) Combined effect of total nucleated cell dose and HLA match on transplantation outcome in 1061 cord blood recipients with hematologic malignancies. Blood 115:1843–1849

    Article  PubMed  CAS  Google Scholar 

  18. Rocha V, Cornish J, Sievers EL et al (2001) Comparison of outcomes of unrelated bone marrow and umbilical cord blood transplants in children with acute leukemia. Blood 97:2962–2971

    Article  PubMed  CAS  Google Scholar 

  19. Childs R, Clave E, Contentin N et al (1999) Engraftment kinetics after nonmyeloablative allogeneic peripheral blood stem cell transplantation: full donor T-cell chimerism precedes alloimmune responses. Blood 94:3234–3241

    PubMed  CAS  Google Scholar 

  20. Urbano-Ispizua A, Rozman C, Pimentel P et al (2001) Spanish Group for Allogenic Peripheral Blood Transplan­tation. The number of donor CD3(+) cells is the most important factor for graft failure after allogeneic transplantation of CD34(+) selected cells from peripheral blood from HLA-identical siblings. Blood 97:383–387

    Article  PubMed  CAS  Google Scholar 

  21. Baron F, Baker JE, Storb R, Gooley TA, Sandmaier BM, Maris MB, Maloney DG, Heimfeld S, Oparin D, Zellmer E, Radich JP, Grumet FC, Blume KG, Chauncey TR, Little MT (2004) Kinetics of engraftment in patients with hematologic malignancies given allogeneic hematopoietic cell transplantation after nonmyeloablative conditioning. Blood 104:2254–2262

    Article  PubMed  CAS  Google Scholar 

  22. Lion T, Daxberger H, Dobovsky J, Filipcik P, Fritsch G, Printz D, Peters C, Matthes-Martin S, Lawitschka A, Gadner H (2001) Analysis of chimerism within specific leukocyte subsets for detection of residual or recurrent leukemia in pediatric patients after allogeneic stem cell transplantation. Leukemia 15:307–310

    Article  PubMed  CAS  Google Scholar 

  23. Luhm RA, Bellissimo DB, Uzgiris AJ, Drobyski WR, Hessner MJ (2000) Quantitative evaluation of post-bone ­marrow ­transplant engraftment status using fluorescent-labeled variable number of tandem repeats. Mol Diagn 5:129–138

    Article  PubMed  CAS  Google Scholar 

  24. Lins AM, Sprecher CJ, Puers C, Schumm JW (1996) Multiplex sets for the amplification of polymorphic short tandem repeat loci–silver stain and fluorescence detection. Biotechniques 20:882–889

    PubMed  CAS  Google Scholar 

  25. Scharf SJ, Smith AG, Hansen JA, McFarland C, Erlich HA (1995) Quantitative determination of bone marrow transplant engraftment using fluorescent polymerase chain reaction primers for human identity markers. Blood 85:1954–1963

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 525 East 68th Street, New York, NY, 10065, USA

    Hanna Rennert & Debra G. B. Leonard

  2. Department of Medicine, Weill Cornell Medical College, 520 East 70th St., New York, NY, 10021, USA

    Tsiporah Shore

Authors
  1. Hanna Rennert
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Debra G. B. Leonard
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tsiporah Shore
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hanna Rennert .

Editor information

Editors and Affiliations

  1. , Department of Pathology, Stanford University School of Medicine, Pasteur Drive 300, Stanford, 94305, California, USA

    Iris Schrijver

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Rennert, H., Leonard, D.G.B., Shore, T. (2011). Bone Marrow Engraftment Analysis. In: Schrijver, I. (eds) Diagnostic Molecular Pathology in Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19677-5_19

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-19677-5_19

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-19676-8

  • Online ISBN: 978-3-642-19677-5

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation