Abstract
Development of RNA cleaving agents, artificial RNases, has attracted considerable attention in the last years, because they can find important applications in biotechnology, for manipulating RNA, and in drug design. A direct approach to design of artificial RNases consists in imitation of the active centers of natural enzymes by simple organic structures possessing minimal sets of the most important characteristics that are essential for catalysis (for reviews see Vlassov et al. 1998; Komiyama and Sumaoka 1998). Usually, these primitive mimics are less efficient and less specific as compared to the natural enzymes. However, these synthetic constructs are more stable than proteins and can function in a broader range of conditions. Artificial RNases are much smaller than the natural enzymes and in contrast to the natural enzymes, their activity is not modulated by different effector molecules. This simplicity appears to be an advantage when artificial RNases are used in design of therapeutics for controlling gene expression or as components of synthetic conjugates. These conjugates are targeted to specific RNA molecules by oligonucleotides, and unwanted interactions of their components have to be minimized.
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Vlassov, V.V., Vlassov, A.V. (2004). Cleavage of RNA by Imidazole. In: Zenkova, M.A. (eds) Artificial Nucleases. Nucleic Acids and Molecular Biology, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18510-6_5
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DOI: https://doi.org/10.1007/978-3-642-18510-6_5
Publisher Name: Springer, Berlin, Heidelberg
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