Reconstructing the Phylogenetic Relationships of the Cyclophyllidean Cestodes: A Case Study Using ITS2 rDNA and Sequence-Structure Alignment

  • Veena Tandon
  • Devendra Kumar Biswal
  • Pramod Kumar Prasad
  • Chenkual Malsawmtluangi
Part of the Communications in Computer and Information Science book series (CCIS, volume 127)


Different authors have subscribed to varied opinions on the phylogenetic relationships and systematic position of cyclophyllidean cestodes owing to high degree of non-significant morphological variations among these taxa. Molecular phylogenetic analysis based on ITS2 rDNA of 16 representatives spanning 6 different families (Mesocestoididae, Davaineidae, Anoplocephalidae, Taeniidae, Dipylidiidae and Hymenolepididae) of the Order Cyclophyllidea and one out group from the family Diphyllobothriidae of the Order Pseudophyllidea revealed the order Order Cyclophyllidea to be a monophyletic clade. The results were further validated by bayesian analysis, primary sequence-structure alignment and subsequent molecular morphometrics analysis. A consistent support for all the major nodes was seen across all the phylogenetic trees. Interestingly, the genus Mesocestoides, in which the genital pores are ventral in position, a feature of the Order Pseudophyllidea, was accommodated within Cyclophyllidea and served as a sister clade close to the families Taeniidae, Anoplocephalidae, Hymenolepididae and Dipylidiidae.


Mesocestoides Cyclophyllidea cestodes ITS2 rDNA Molecular morphometrics Sequence-structure alignment 


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  1. 1.
    Mariaux, J.: Cestode systematics: Any progress? Int. J. Parasitol. 26(3), 231–243 (1996)CrossRefGoogle Scholar
  2. 2.
    Rausch, R.L.: Family Mesocestoididae Fuhrmann, 1907. In: Khalil, L.F., Jones, A., Bray, R.A. (eds.) Keys to the Cestode Parasites of Vertebrates, pp. 309–314. CAB International, Wallingford (1994)Google Scholar
  3. 3.
    Chandler, A.C.: Observations on the anatomy of Mesocestoides. J. Parasitol. 32, 242–246 (1946)CrossRefGoogle Scholar
  4. 4.
    Kamegai, S., Ichihara, A., Nonobe, H., Machida, M.: The 6th and 7th records of human infection with Mesocestoides lineatus (Cestoda) in Japan. Research Bulletin of the Meguro Parasitological Museum 1, 1–7 (1967)Google Scholar
  5. 5.
    von Nickisch-Rosenegk, M., Lucius, R., Loos-Frank, B.: Contributions to the phylogeny of the Cyclophyllidea (Cestoda) inferred from mitochondrial 12S rDNA. J. Mol. Evol. 48(5), 586–596 (1999)CrossRefGoogle Scholar
  6. 6.
    Coleman, A.W.: ITS2 is a double-edged tool for eukaryote evolutionary comparisons. Trends Genet. 19(7), 370–375 (2003)CrossRefGoogle Scholar
  7. 7.
    Schultz, J., Maisel, S., Gerlach, D., Müller, T., Wolf, M.: A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout the Eukaryota. RNA 11(4), 361–364 (2005)CrossRefGoogle Scholar
  8. 8.
    Billoud, B., Guerrucci, M.A., Masselot, M., Deutsch, J.S.: Cirripede phylogeny using a novel approach: molecular morphometrics. Mol. Biol. Evol. 17(10), 1435–1445 (2000)Google Scholar
  9. 9.
    Thompson, J.D., Higgins, D.G., Gibson, T.J.: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673–4680 (1994)CrossRefGoogle Scholar
  10. 10.
    Tamura, K., Dudley, J., Nei, M., Kumar, S.: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24(8), 1596–1599 (2007)CrossRefGoogle Scholar
  11. 11.
    Ronquist, F., Huelsenbeck, J.F.: MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574 (2003)CrossRefGoogle Scholar
  12. 12.
    Page, R.D.: TreeView: an application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 12, 357–358 (1996)Google Scholar
  13. 13.
    Zuker, M.: Mfold web server for nucleic folding and hybridization prediction. Nucleic Acids Res. 31, 3406–3415 (2003)CrossRefGoogle Scholar
  14. 14.
    Siebert, S., Backofen, R.: MARNA:multiple alignment and consensus structure prediction of RNAs based on sequence structure comparisons. Bioinformatics 21, 3352–3359 (2005)CrossRefGoogle Scholar
  15. 15.
    Wolf, M., Ruderisch, B., Dandekar, T., Schultz, J., Müller, T.: ProfDistS (profile-) distance based phylogeny on sequence-structure alignments. Bioinformatics 24(20), 2401–2402 (2008)CrossRefGoogle Scholar
  16. 16.
    Saitou, N., Nei, M.: The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425 (1987)Google Scholar
  17. 17.
    Tamura, K., Dudley, J., Nei, M., Kumar, S.: Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc. Natl. Acad. Sci. USA 101, 11030–11035 (2004)CrossRefGoogle Scholar
  18. 18.
    Dayhoff, M.O., Eck, R.V., Chang, M.A., Sochard, M.R.: Atlas of Protein Sequence and Structure. National Biomedical Research Foundation, Silver Spring, Maryland (1965)Google Scholar
  19. 19.
    Nei, M., Kumar, S.: Molecular Evolution and Phylogenetics. Oxford University Press, New York (2000)Google Scholar
  20. 20.
    Cote, C.A., Peculis, B.A.: Role of the ITS 2-proximal stem and evidence for indirect recognition of processing sites in pre-rRNA processing in yeast. Nucleic Acids Res. 29, 2106–2116 (2001)CrossRefGoogle Scholar
  21. 21.
    Brooks, D.R., Hoberg, E.P., Weekes, P.J.: Preliminary phylogenetic systematic analysis of the major lineages of the Eucestoda (Platyhelminthes: Cercomeria). Proceedings of the Biological Society of Washington 104, 651–688 (1991)Google Scholar
  22. 22.
    Smith, A.B., Lafay, B., Christen, R.: Comparative variation of morphological and molecular evolution through geologic time: 28S ribosomal RNA versus morphology in echinoids. Philos. Trans. R. Soc. Lond. B Biol. Sci. 338, 365–382 (1992)CrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Veena Tandon
    • 1
    • 2
  • Devendra Kumar Biswal
    • 2
  • Pramod Kumar Prasad
    • 1
  • Chenkual Malsawmtluangi
    • 1
  1. 1.Department of ZoologyNorth-Eastern Hill UniversityShillongIndia
  2. 2.Bioinformatics CentreNorth-Eastern Hill UniversityShillongIndia

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