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Expression of AC133 in Childhood Acute Leukemia

  • Conference paper
Acute Leukemias VIII

Part of the book series: Haematology and Blood Transfusion / Hämatologie und Bluttransfusion ((HAEMATOLOGY,volume 40))

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Abstract

The antigen AC 133 is a novel 5-transmembrane cell surface molecule with a molecular weight if 117-120 kD. It is selectively expressed on a subset of CD34+ human hematopoietic stem and progenitor cells. The monoclonal antibodies ACV133/1 and AC 133/2 recognize early progenitor cells derived from peripheral blood and bone marrow and provide an alternative to conventionally applied anti-CD34 antibodies used for the selection and characterization of cells necessary for short- and long term engraftment in transplant situation.

In the present study we analysed bone marrow of leukemic blast cells at the time of initial diagnosis and in relapse (n = 28; 19 ALLs, 9 AMLs) just as various leukemia derived cell lines (i.e. KG1, KGla, Molt-4, Jurkat) using flow cytometry.

Additionally we evaluated the expression of AC 133 bs. CD34 in umbilical cord blood (UCB, n = 43), peripheral blood (PB) from healthy donors (n = 10) and peripheral blood from patients (neuroblastoma, rhabdomyosarcoma and leukemia) undergoing mobilization with the cytokine G-CSF (n = 34) and from leukapheresis products (n = 15) as well as CD34+- or AC133+-selected cell populations (n = 6) following MiniMACS- or VarioMACS-System (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were stained with the MoAbs anti-AC133-PE (Miltenyi Biotec) and anti-CD34-PE/-FITC (clone HPCA-2/BD Heidelberg).

Our results so far obtained by standard flow cytometry demonstrate that only CD34+ stem- and progenitor cells originated from different sources were exclusively positive for AC 133 antigen. In contrast to this observation we found in acute leukemias with CD34+-phenotype (74%) high or partial coexpression of AC133 in 13/19 cases (68%). These data have shown, that about 30% of the phenotyped acute leukemias had no detectable levels of AC 133 molecules as well as for the myeloid leukemia cell line KG 1a (CD34+/AC133+).

On the basis of our results we believe than the usage of anti-AC133 applying positive selection with MACS-technology proves to be of great benefit in order to separate contaminating CD34+ /AC133+ tumor cells from leukapheresis harvests.

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Authors and Affiliations

  1. Department of Hematology and Oncology, J. W. Goethe University, Clinic of Pediatrics III, D-60590, Frankfurt/M., Germany

    U. Ebener, S. Wehner, A. Brinkmann, E. Niegemann & B. Kornhuber

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  1. U. Ebener
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  2. S. Wehner
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  3. A. Brinkmann
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  4. E. Niegemann
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  5. B. Kornhuber
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Editor information

Editors and Affiliations

  1. Department of Internal Medicine A, Germany

    T. Büchner

  2. Department of Internal Medicine III, University Hospital Großhadern Ludwig-Maximilians-University, Marchioninistr. 15, 81377, München, Germany

    W. Hiddemann

  3. Dept. of Internal Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075, Göttingen, Germany

    B. Wörmann

  4. Department of Pediatrics, University of Münster, Albert-Schweitzer-Straße 33, 48149, Münster, Germany

    G. Schellong , J. Ritter  & U. Creutzig ,  & 

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Ebener, U., Wehner, S., Brinkmann, A., Niegemann, E., Kornhuber, B. (2001). Expression of AC133 in Childhood Acute Leukemia. In: Büchner, T., Hiddemann, W., Wörmann, B., Schellong, G., Ritter, J., Creutzig, U. (eds) Acute Leukemias VIII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 40. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18156-6_12

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  • DOI: https://doi.org/10.1007/978-3-642-18156-6_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62109-3

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