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Targeting Functional Centers of the Ribosome

Part of the book series: Springer Theses ((Springer Theses))

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Abstract

Complete crystallographic data sets of complexes of D50S with SB-571519, SB-280080, and retapamulin (SB-275833) yielded electron density maps at 3.50, 3.56, and 3.66 Å resolution, respectively, in which the location and conformation of each of the three bound compounds were unambiguously resolved. Grouped occupancy refinement yielded a value of approximately 1.0 for all three compounds, confirming that they are quantitatively bound, in accord with the high binding affinity observed in a competitive ribosome-binding assay by using a radiolabeled pleuromutilin derivative.

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Authors and Affiliations

  1. Department of Structural Biology, Weizmann Institute of Science, Herzl Street, 76100, Rehovot, Israel

    Chen Davidovich

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Correspondence to Chen Davidovich .

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© 2011 Springer-Verlag Berlin Heidelberg

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Davidovich, C. (2011). Results. In: Targeting Functional Centers of the Ribosome. Springer Theses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16931-1_3

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