Analysis of Length and Orientation of Microtubules in Wide-Field Fluorescence Microscopy

  • Gerlind Herberich
  • Anca Ivanescu
  • Ivonne Gamper
  • Antonio Sechi
  • Til Aach
Part of the Lecture Notes in Computer Science book series (LNCS, volume 6376)


In this paper we present a novel approach for the analysis of microtubules in wide-field fluorescence microscopy. Microtubules are flexible elongated structures and part of the cytoskeleton, a cytoplasmic scaffolding responsible for cell stability and motility. The method allows for precise measurements of microtubule length and orientation under different conditions despite a high variability of image data and in the presence of artefacts. Application of the proposed method to demonstrate the effect of the protein GAR22 on the rate of polymerisation of microtubules illustrates the potential of our approach.


Segmentation Result High Dynamic Range Directional Cell Motility Microtubule Length Ridge Detector 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Gerlind Herberich
    • 1
  • Anca Ivanescu
    • 1
  • Ivonne Gamper
    • 2
  • Antonio Sechi
    • 2
  • Til Aach
    • 1
  1. 1.Institute of Imaging and Computer VisionRWTH Aachen UniversityGermany
  2. 2.Institute of Biomedical Engineering Dept. of Cell BiologyRWTH Aachen UniversityGermany

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