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Optical Fluorescence Microscopy pp 153–163Cite as

Measuring Molecular Dynamics by FRAP, FCS, and SPT

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Abstract

In many research areas such as cell biology and drug delivery, it is important to obtain quantitative information on dynamic properties of molecules and nanoparticulate matter in biomaterials. Several complementary advanced fluorescence microscopy techniques have been developed for studying the mobility of molecules and particles on the micro- and nanoscale. In this chapter, the most important techniques are discussed: fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS), and single particle tracking (SPT).

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Authors and Affiliations

  1. Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium

    Kevin Braeckmans, Hendrik Deschout, Jo Demeester & Stefaan C. De Smedt

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  1. Kevin Braeckmans
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  2. Hendrik Deschout
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  3. Jo Demeester
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  4. Stefaan C. De Smedt
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Correspondence to Kevin Braeckmans .

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Editors and Affiliations

  1. , Nanophysics Unit, The Italian Institute of Technology - II, Via Morego 30, Genova, 16163, Italy

    Alberto Diaspro

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Braeckmans, K., Deschout, H., Demeester, J., De Smedt, S.C. (2011). Measuring Molecular Dynamics by FRAP, FCS, and SPT. In: Diaspro, A. (eds) Optical Fluorescence Microscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15175-0_9

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