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Nanoscale “DNA Baskets” for the Delivery of siRNA

  • A. C. Zirzow
  • M. Skoblov
  • A. Patanarut
  • C. Smith
  • A. Fisher
  • V. Chandhoke
  • A. Baranova
Part of the IFMBE Proceedings book series (IFMBE, volume 32)

Abstract

Silencing of gene expression by small interfering RNA (siRNA) is promising for drug target discovery and as a therapy. However, a major impediment to the practical use of this technology is an inherent instability of siRNA in the bloodstream, partly due to susceptibility to nucleases. To address this restriction, we evaluated a novel DNA/albumin-based siRNA delivery vehicle that forms a “basket” surrounding the siRNA and provides both steric separation of siRNA from nucleases and local excess of the substrate for nuclease action; thus, slowing the rate of the degradation of siRNA. We found that variation of the albumin concentration in basket construction can significantly decrease the mean size of the basket. Smaller siRNA-containing DNA baskets may increase cellular uptake. We found that the degradation of siRNA is delayed when siRNA is prepared with this delivery vehicle, implying that DNA baskets are a promising technology for further development as the delivery vehicle for siRNA therapeutics.

Keywords

Human Serum Albumin Delivery Vehicle Bovine Serum Albumin Concentration Drug Target Discovery Local Excess 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • A. C. Zirzow
    • 1
  • M. Skoblov
    • 2
  • A. Patanarut
    • 3
  • C. Smith
    • 4
  • A. Fisher
    • 4
  • V. Chandhoke
    • 1
  • A. Baranova
    • 1
    • 2
  1. 1.Department of Molecular an Microbiology, College of ScienceGeorge Mason UniversityFairfaxUSA
  2. 2.Research Center for Medical Genetics, RAMSMoscowRussian Federation
  3. 3.Department of Chemistry and Biochemistry, College of ScienceGeorge Mason UniversityFairfaxUSA
  4. 4.U.S. Army Engineer Research and Development Center-Geospatial Research and Engineering DivisionAlexandriaUSA

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