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Effects of Altered Gravity on the Actin and Microtubule Cytoskeleton, Cell Migration and Neurite Outgrowth

  • Meike Wiedemann
  • Florian P. M. Kohn
  • Harald Roesner
  • Wolfgang R. L. Hanke
Part of the Nonlinear Physical Science book series (NPS)

Abstract

Human SH-SY5Y neuroblastoma cells were used to study the effects of altered gravity on the actin and microtubule cytoskeleton dynamics. A cholinergic stimulation of the cells during a 6-min period of changing gravity (3 parabolas) resulted in an enhanced actin-driven protrusion of evoked lamellipodia. Likewise, the spontaneous protrusive activity of non-activated cells was promoted during exposure to changing gravity (6 up to 31 parabolas). Ground-based experiments revealed a similar enhancement of the spontaneous and an evoked lamellar protrusive activity when the cells were kept at 2g hyper—gravity for at least 6 min. This gravity response was independent of the direction of the acceleration vector in respect to the cells. Exposure of the cells to “simulated weightlessness” (clinorotation) had no obvious influence on this type of lamellar actin cytoskeleton dynamics.

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Copyright information

© Higher Education Press, Beijing and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Meike Wiedemann
    • 1
  • Florian P. M. Kohn
    • 1
  • Harald Roesner
    • 2
  • Wolfgang R. L. Hanke
    • 1
  1. 1.Department of Physiology (230)University of Hohenheim MembramephysiologyStuttgartGermany
  2. 2.Department of ZoologyUniversity of HohenheimStuttgartGermany

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