Production of Ligninolytic Enzymes by White-rot Fungi during Bioremediation of Oil-contaminated Soil
A number of white-rot fungi, including strains of Pleurotus ostreatus, Lentinus edodes, Coriolus sp., and Agaricus sp., were tested for the production of ligninolytic enzymes during decontamination of old oil-polluted industrial soil. The solid-state cultures of all studied fungi were able to grow and produce ligninolytic enzymes in the soil. The growth rates, the mycelium densities, the production of ligninolytic enzymes, and the degradation of old oil contamination decreased in the order Agaricus sp. > P. ostreatus > L. edodes > Coriolus sp. The most active producers of laccase and peroxidase were the strains of P. ostreatus and Agaricus sp. The production of peroxidase by the strains of L. edodes and Coriolus sp. was not found under the conditions used. The fungi that actively colonized the soil and produced ligninolytic enzymes also actively degraded old oil contamination in the soil.
KeywordsLaccase Activity Ligninolytic Enzyme Pleurotus Ostreatus Nonsterile Soil Versatile Peroxidase
We are grateful to Dmitry N. Tychinin for his assistance in the preparation of the English text of this paper.
This work was supported by the federal target-oriented program “Issledovaniya i razrabotki po prioritetnym napravleniyam razvitiya nauchno-tekhnologicheskogo kompleksa Rossii na 2007–2012 gody” (Research and Developments on Priority Directions in the Development of Russia’s Science and Technology Complex for 2007–2012), State contract no. 02.512.11.2210.
- Barr DP, Aust SD (1994) Mechanisms white-rot fungi use to degrade pollutants. Environ Sci Technol 28:79A–87AGoogle Scholar
- Soils. GOST 26483–85. (Soils. Preparation of salt extract and determination of its pH by SINAO Method) (in Russian)Google Scholar
- Gramss G (2000) Degradation of aromatic xenobiotics in aerated soils by enzyme systems of microorganisms and plants. In: Wise DL, Trantolo DJ, Cichon EJ, Inyang HI, Stottmeister U (eds) Bioremediation of contaminated soils. Marcel Dekker, New York, pp 489–536Google Scholar
- Polunina AG, Kushik GI (1977) Metody analiza organicheskogo veshchestva porod, nefti i gaza (Methods of Analyslis of Organic Matter in Rocks, Oil, and Gas). In: Ryl’kov AV (ed) Tyumen’: Tr Zap-Sib. NIGNI, 122 (in Russian)Google Scholar
- Pozdnyakova N, Dubrovskaya E, Makarov O, Turkovskaya O (2008a) Use of the complex “fungus Agaricus sp – indigenous microflora” in PAH degradation. In: Proceedings of the All-Russia conference “Basic and applied aspects of studies of symbiotic systems”. p 30 (in Russian)Google Scholar
- Sasek V, Cajthaml T, Bhatt M (2003) Use of fungal technology in soil remediation: a case study. Water Air Soil Pollut Focus 3:5–14Google Scholar
- Stanley GA, Britz ML, Boonchan S, Juhasz A (2000) Detoxification of soil containing high-molecular weight polycyclic aromatic hydrocarbons by Gram-negative bacteria and bacterial-fungal cocultures. In: Wise DL, Trantglo DJ, Cichon EJ, Inyang HI, Stottmeister U (eds) Bioremediation of contaminated soils. Marcel Dekker, New York, pp 409–443Google Scholar