Production of Ligninolytic Enzymes by White-rot Fungi during Bioremediation of Oil-contaminated Soil

  • Natalia N. PozdnyakovaEmail author
  • Ekaterina V. Dubrovskaya
  • Oleg E. Makarov
  • Valentina E. Nikitina
  • Olga V. Turkovskaya
Part of the Soil Biology book series (SOILBIOL, volume 22)


A number of white-rot fungi, including strains of Pleurotus ostreatus, Lentinus edodes, Coriolus sp., and Agaricus sp., were tested for the production of ligninolytic enzymes during decontamination of old oil-polluted industrial soil. The solid-state cultures of all studied fungi were able to grow and produce ligninolytic enzymes in the soil. The growth rates, the mycelium densities, the production of ligninolytic enzymes, and the degradation of old oil contamination decreased in the order Agaricus sp. > P. ostreatus > L. edodes > Coriolus sp. The most active producers of laccase and peroxidase were the strains of P. ostreatus and Agaricus sp. The production of peroxidase by the strains of L. edodes and Coriolus sp. was not found under the conditions used. The fungi that actively colonized the soil and produced ligninolytic enzymes also actively degraded old oil contamination in the soil.


Laccase Activity Ligninolytic Enzyme Pleurotus Ostreatus Nonsterile Soil Versatile Peroxidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are grateful to Dmitry N. Tychinin for his assistance in the preparation of the English text of this paper.

This work was supported by the federal target-oriented program “Issledovaniya i razrabotki po prioritetnym napravleniyam razvitiya nauchno-tekhnologicheskogo kompleksa Rossii na 2007–2012 gody” (Research and Developments on Priority Directions in the Development of Russia’s Science and Technology Complex for 2007–2012), State contract no. 02.512.11.2210.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Natalia N. Pozdnyakova
    • 1
    Email author
  • Ekaterina V. Dubrovskaya
    • 2
  • Oleg E. Makarov
    • 2
  • Valentina E. Nikitina
    • 2
  • Olga V. Turkovskaya
    • 2
  1. 1.Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Academy of SciencesSaratovRussia
  2. 2.Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Academy of SciencesSaratovRussia

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