Role of Enzymes in Maintaining Soil Health

  • Shonkor Kumar DasEmail author
  • Ajit Varma
Part of the Soil Biology book series (SOILBIOL, volume 22)


Soil enzymes are constantly playing vital roles for the maintenance of soil ecology and soil health. These enzymatic activities in the soil are mainly of microbial origin, being derived from intracellular, cell-associated or free enzymes. Therefore, microorganisms are acting as the indicators of soil health, as they have active effects on nutritional cycling, also affecting the physical and chemical properties of soil. Microorganisms respond quickly even to minute changes by changing their population and activities, and thus, can be used for soil health assessment. On the other hand, soil enzymes are the direct mediators for biological catabolism of soil organic and mineral components and they are often closely related to soil organic matters, soil physical properties, and microbial activities or biomass. They are the better indicators of soil health as changes of enzymes are much sooner than other parameters, thus providing early indications of changes in soil health. In addition, their activities can be used as the measures of microbial activity, soil productivity, and inhibiting effects of pollutants. The potential enzymes playing major roles in maintaining soil health are – amylase, arylsulphatase, β-glucosidase, cellulase, chitinase, dehydrogenase, phosphatase, protease, and urease. Deterioration of soil, and thereby soil health, is of concern for human, animal, and plant health because air, groundwater, and surface water consumed by humans can be adversely affected by mismanaged and contaminated soil.


Soil Organic Matter Urease Activity Soil Enzyme Soil Enzyme Activity Soil Health 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Acosta-Martínez V, Tabatabai MA (2000) Enzyme activities in a limed agricultural soil. Biol Fertil Soils 31:85–91CrossRefGoogle Scholar
  2. Acton DF, Gregorich EG (1995) Executive summary. In: Acton DF, Gregorich EG (eds) The health of our soils. Towards sustainable agriculture in Canada. Center for Land and Biological Resources, Research Branch Agriculture and Agri-food Canada, ON, CanadaCrossRefGoogle Scholar
  3. Ajwa HA, Tabatabai MA (1994) Decomposition of different organic materials in soils. Biol Fertil Soils 18:175–182CrossRefGoogle Scholar
  4. Alf K, Nannipieri P (1995) Cellulase activity, methods in applied soil microbiology and biochemistry. Academic, LondonGoogle Scholar
  5. Andrews RK, Blakeley RL, Zerner B (1989) Urease: a Ni (II) metalloenzyme. In: Lancaster JR (ed) The bioinorganic chemistry of nickel. VCH, New York, pp 141–166Google Scholar
  6. Arinze AE, Yubedee AG (2000) Effect of fungicides on Fusarium grain rot and enzyme production in maize (Zea mays L.). Glob J Appl Sci 6:629–634Google Scholar
  7. Atlas RM, Pramer D, Bartha R (1978) Assessment of pesticide effects on non-target soil microorganisms. Soil Biol Biochem 10:231–239CrossRefGoogle Scholar
  8. Bandick AK, Dick RP (1999) Field management effects on soil enzyme activities. Soil Biol Biochem 31:1471–1479CrossRefGoogle Scholar
  9. Baruah M, Mishra RR (1986) Effect of herbicides butachlor, 2,4-d and oxyfluorfen on enzyme activities and CO2 evolution in submerged paddy field soil. Plant Soil 96:287–291CrossRefGoogle Scholar
  10. Benckiser G, Santiago S, Neue HU, Watanabe I, Ottow JCG (1984) Effect of fertilization and exudation, dehydrogenase activity, iron-reducing populations and Fe2+ formation in the rhizosphere of rice (Oryza sativa L.) in relation to iron toxicity. Plant Soil 79:305–316CrossRefGoogle Scholar
  11. Bloem J, de Ruiter P, Bouwman LA (1997) Food webs and nutrient cycling in agro-ecosystems. In: Van Elsas JD, Trevors JT, Wellington EMH (eds) Modern soil microbiology. Marcel Dekker, New York, pp 245–278Google Scholar
  12. Borner H (1958) Untersuchungen uber den Abbau von Phlorizin im Boden. Ein Beitrag zum Problem der Bodenmudigkeit bei Obstgeholzen. Naturwiss 45:138–139CrossRefGoogle Scholar
  13. Bromfield SM (1954) Reduction of ferric compounds by soil bacteria. J Gen Microbiol 11:1–6PubMedGoogle Scholar
  14. Brzezinska M, Stepniewska Z, Stepniewski W (1998) Soil oxygen status and dehydrogenase activity. Soil Biol Biochem 30:1783–1790CrossRefGoogle Scholar
  15. Burns RG (1978) Enzyme activity in soil: some theoretical and practical considerations. In: Bums RG (ed) Soil enzymes. Academic, London, pp 295–340Google Scholar
  16. Burns RG (1982) Enzyme activity in soil: location and possible role in microbial ecology. Soil Biol Biochem 14:423–427CrossRefGoogle Scholar
  17. Burns RG (1986) Interaction of enzymes with soil mineral and organic colloids. In: Huang PM, Schnitzer M (eds) Interactions of soil minerals with natural organics and microbes. Soil Science Society of America, Madison, pp 429–452Google Scholar
  18. Byrnes BH, Amberger A (1989) Fate of broadcast urea in a flooded soil when treated with N-(n-butyl) thiophospheric triamide, a urease inhibitor. Fertil Res 18:221–231CrossRefGoogle Scholar
  19. Chet I (1987) Trichoderma-application, mode of action, and potential as biocontrol agent of soil borne pathogenic fungi. In: Chet I (ed) Innovative approaches to plant disease control. Wiley, New York, pp 137–349Google Scholar
  20. Chet I, Henis Y (1975) Sclerotial morphogenesis in fungi. Annu Rev Phytopathol 13:169–192CrossRefGoogle Scholar
  21. Chet I, Ordentlich A, Shapira R, Oppenheim A (1990) Mechanism of biocontrol of soil borne plant pathogen by rhizobacteria. Plant Soil 129:85–92CrossRefGoogle Scholar
  22. Deng SP, Tabatabai MA (1994) Cellulase activity of soils. Soil Biol Biochem 26:1347–1354CrossRefGoogle Scholar
  23. Deshpande MV (1986) Enzymatic degradation of chitin and its biological applications. J Sci Ind Res 45:273–281Google Scholar
  24. Dick RP, Sandor JA, Eash NS (1994) Soil enzyme activities after 1500 years of terrace agriculture in the Colca Valley. Peru Agric Ecosyst Environ 50:123–131CrossRefGoogle Scholar
  25. Dick RP, Breakwell DP, Turco RF (1996) Soil enzyme activities and biodiversity measurements as integrative microbiological indicators. In: Doran JW, Jones AJ (eds) Methods of assessing soil quality. Soil Science Society of America, Madison, WI, pp 247–271Google Scholar
  26. Dick RP (1997) Soil enzyme activities as integrative indicators of soil health. In: Pankhurst CE, Doube BM, Gupta VVSR (eds) Biological indicators of soil health. CABI, Wellingford, pp 121–156Google Scholar
  27. Dick WA, Cheng L, Wang P (2000) Soil acid and alkaline phosphatase activity as pH adjustment indicators. Soil Biol Biochem 32:1915–1919CrossRefGoogle Scholar
  28. Dodgson KS, White G, Fitzgerald JW (1982) Sulphatase enzyme of microbial origin. Afr J Biotechnol Vol I. CRC, FL, pp 156–159Google Scholar
  29. Doran JW, Parkin TB (1994) Defining and assessing soil quality. In: Doran JW, Coleman DC, Bezdicek DF, Stewart BA (eds) Defining soil quality for a sustainable environment. Soil Science Society of America, Madison, pp 3–21Google Scholar
  30. Doran JW, Safley M (1997) Defining and assessing soil health and sustainable productivity. In: Pankhurst CE, Doube BM, Gupta VVSR (eds) Biological indicators of soil health. CABI, Wellingford, pp 1–28Google Scholar
  31. Eivazi F, Tabatabai MA (1977) Phosphates in soils. Soil Biol Biochem 9:167–172CrossRefGoogle Scholar
  32. Eivazi F, Tabatabai MA (1988) Glucosidases and galactosidases in soils. Soil Biol Biochem 20:601–606CrossRefGoogle Scholar
  33. Ellert BH, Clapperton MJ, Anderson DW (1997) An ecosystem perspective of soil quality. In: Gregorich EG, Carter MR (eds) Soil quality for crop production and ecosystem health. Elsevier, Amsterdam, pp 115–141CrossRefGoogle Scholar
  34. Elliott LF, Lynch JM, Papendick RI (1996) The microbial component of soil quality. In: Stotzky G, Bollag JM (eds) Soil biochemistry. Marcel Dekker, New York, pp 1–21Google Scholar
  35. Eriksson KEL, Blancbette RA, Ander P (1990) Biodegration of cellulose. In: Eriksson KEL, Blanchette RA, Ander P (eds) Microbial and enzymatic degradation of wood and wood components. Springer, New York, pp 89–180CrossRefGoogle Scholar
  36. Esen A (1993) β-glucosidases: overview. In: Esen A (ed) β-glucosidases and molecular biology. American Chemical Society, Washington, DC, pp 9–17CrossRefGoogle Scholar
  37. Frank T, Malkomes HP (1993) Influence of temperature on microbial activities and their reaction to the herbicide Goltix in different soils under laboratory conditions. Zentralblatt für Mikrobiol 148:403–412Google Scholar
  38. Galstian AS, Awungian ZS (1974) Significance of the enzymes in oxidation of Fe and Mn oxides in soil (in Russian). Trans. 10th Intern. Congress Soil Sci III. Nauka Publishing House, Moscow, pp 130–135Google Scholar
  39. Ganeshamurthy AM, Singh G, Singh NT (1995) Sulphur status and response of rice to sulphur on some soils of Andaman and Nicobar Islands. J Indian Soc Soil Sci 43:637–641Google Scholar
  40. Garcia C, Hernández T (1997) Biological and biochemical indicators in derelict soils subject to erosion. Soil Biol Biochem 29:171–177CrossRefGoogle Scholar
  41. Glinski J, Stepniewski W (1985) Soil aeration and its role for plants. CRC, Boca Raton, FLGoogle Scholar
  42. Gupta VVSR, Farrell RE, Germida JJ (1993) Activity of arylsuphatases in Saskatchewan soils. Can J Soil Sci 73:341–347CrossRefGoogle Scholar
  43. Halvorson JJ, Smith JL, Papendick RI (1997) Issues of scale for evaluating soil quality. J Soil Water Conserv 52:26–30Google Scholar
  44. James ES, Russel LW, Mitrick A (1991) Phosphate stress response in hydroponically grown maize. Plant Soil 132:85–90CrossRefGoogle Scholar
  45. Kandeler E (1996) Nitrate. In: Schinner F, Öhlinger R, Kandeler E, Margesin R (eds) Methods in soil biology. Springer, Berlin, pp 408–410Google Scholar
  46. Kanfer JN, Mumford RA, Raghavan SS, Byrd J (1974) Purification of β-glucosidase activities from bovine spleen affinity chromatography. Anal Biochem 60:200–205PubMedCrossRefGoogle Scholar
  47. Karthikeyan AS, Varadarajan DK, Mukatira UT, D’Urzo MP, Damaz B, Raghothama KG (2002) Regulated expression of Arabidopsis phosphate transporters. Plant Physiol 130:221–233PubMedCrossRefGoogle Scholar
  48. Kennedy AC, Papendick RI (1995) Microbial characteristics of soil quality. J soil water conserv. May–June:243–248Google Scholar
  49. Kertesz MA, Mirleau P (2004) The role of soil microbes in plant sulphur nutrition. J Exp Bot 55:1939–1945PubMedCrossRefGoogle Scholar
  50. King NJ (1967) Glucoamylase of Coniophora cerebella. Biochem J 105:577–583PubMedGoogle Scholar
  51. Kiss S, Dragan-Bularda M, Radulescu D (1978) Soil polysaccharidases: activity and agricultural importance. In: Burns RG (ed) Soil enzymes. Academic, London, pp 117–147Google Scholar
  52. Klose S, Tabatabai MA (1999) Arylsulphatase activity of microbial biomass in soils. Soil Sci Soc Am J 63:569–574CrossRefGoogle Scholar
  53. Ladd JN, Jackson RB (1982) In: Stevenson FJ (ed) Nitrogen in agricultural soils. American Society of Agronomy, WI, pp 173–228Google Scholar
  54. Madejón E, Burgos P, López R, Cabrera F (2001) Soil enzymatic response to addition of heavy metals with organic residues. Biol Fertil Soils 34:144–150CrossRefGoogle Scholar
  55. Martinez CE, Tabatabai MA (1997) Decomposition of biotechnology by-products in soils. J Environ Qual 26:625–632CrossRefGoogle Scholar
  56. McCarthy GW, Siddaramappa R, Reight RJ, Coddling EE, Gao G (1994) Evaluation of coal combustion by products as soil liming materials: their influence on soil pH and enzyme activities. Biol Fertil Soils 17:167–172CrossRefGoogle Scholar
  57. McGill WB, Colle CV (1981) Comparative aspects of cycling of organic C, N, S and P through soil organic matter. Geoderma 26:267–286CrossRefGoogle Scholar
  58. McLaren AD (1975) Soil as a system of humus and clay immobilised enzymes. Chem Scripta 8:97–99Google Scholar
  59. Miwa T, Ceng CT, Fujisaki M, Toishi A (1937) Zur Frage der Spezifitat der Glykosidasen. I. Verhalted vonβ-d-glucosidases verschiedener Herkunft gegenuberdenβ-d-Glucosiden mit verschiedenen Aglykonen. Acta Phytochim (Tokyo) 10:155–170Google Scholar
  60. Mobley HLT, Hausinger RP (1989) Microbial urease: significance, regulation and molecular characterization. Microbiol Rev 53:85–108PubMedGoogle Scholar
  61. Mudge SR, Rae AL, Diatloff E, Smith FW (2002) Expression analysis suggests novel roles for members of Pht1 family of phosphate transporters in Arabidopsis. Plant J 31:341–353PubMedCrossRefGoogle Scholar
  62. Nannipieri P, Sequi P, Fusi P (1996) Humus and enzyme activity. In: Piccolo A (ed) Humic substances in terrestrial ecosystems. Elsevier, New York, pp 293–328CrossRefGoogle Scholar
  63. Ndiaye EL, Sandeno JM, McGrath D, Dick RP (2000) Integrative biological indicators for detecting change in soil quality. Am J Altern Agric 15:26–36CrossRefGoogle Scholar
  64. Ordentlich A, Elad Y, Chet I (1988) The role of chitinase of Serratia marcescens in biocontrol of Sclerotium rolfsii. Phytopathology 78:84–88Google Scholar
  65. Pancholy SK, Rice EL (1973) Soil enzymes in relation to old field succession; amylase, cellulose, invertase, dehydrogenase and urease. Soil Sci Soc Am J 37:47–50CrossRefGoogle Scholar
  66. Pankhurst CE, Hawke BG, McDonald HJ, Kirkby CA, Buckerfield JC, Michelsen P, O’Brien KA, Gupta VVSR, Doube BM (1995) Evaluation of soil biological properties as potential bioindicators of soil health. Aust J Exp Agric 35:1015–1028CrossRefGoogle Scholar
  67. Pankhurst CE, Doube BM, Gupta VVSR (1997) Biological indicators of soil health: synthesis. In: Pankhurst CE, Doube BM, Gupta VVSR (eds) Biological indicators of soil health. CABI, Wallingford, Oxfordshire, pp 419–435Google Scholar
  68. Park D (1960) Antagonism – the background of soil fungi. In: Parkinson D, Waid JS (eds) The ecology of soil fungi. Liverpool University Press, Liverpool, pp 148–159Google Scholar
  69. Parr JF, Papendick RI, Hornick SB, Meyer RE (1992) Soil quality: attributes and relationship to alternative and sustainable agriculture. Am J Altern Agric 7:5–11CrossRefGoogle Scholar
  70. Patrick ZA (1955) The peach replant problem in Ontario. II. Toxic substances from microbial decomposition products of peach root residues. Can J Bot 33:461–486CrossRefGoogle Scholar
  71. Pazur JH (1965) Enzymes in the synthesis and hydrolysis of starch. In: Whistler R, Paschall EF (eds) Starch: chemistry and technology, vol 1, Fundamental aspects. Academic, New York, pp 133–175Google Scholar
  72. Petker AS, Rai PK (1992) Effect of fungicides on activity, secretion of some extra cellular enzymes and growth of Alternaria alternata. Indian J Appl Pure Biol 7:57–59Google Scholar
  73. Pettit NM, Smith ARJ, Freedman RB, Burns RG (1976) Soil urease: activity, stability and kinetic properties. Soil Biol Biochem 8:479–484CrossRefGoogle Scholar
  74. Pitchel JR, Hayes JM (1990) Influence of fly ash on soil microbial activity and populations. J Environ Qual 19:593–597Google Scholar
  75. Polacco JC (1977) Is nickel a universal component of plant ureases? Plant Sci Lett 10:249–255CrossRefGoogle Scholar
  76. Reddy GB, Faza A (1989) Dehydrogenase activity in sludge amended soil. Soil Biol Biochem 21:327CrossRefGoogle Scholar
  77. Richmond PA (1991) Occurrence and functions of native cellulose. In: Haigler CH, Weimer PJ (eds) Biosynthesis and biodegradation of cellulose. Marcel Dekker, New York, pp 5–23Google Scholar
  78. Ross DJ (1975) Studies on a climosequence of soils in tussock grasslands-5. Invertase and amylase activities of topsoils and their relationships with other properties. NZ J Sci 18:511–518Google Scholar
  79. Ross DJ (1976) Invertase and amylase activities in ryegrass and white clover plants and their relationships with activities in soils under pasture. Soil Biol Biochem 8:351–356CrossRefGoogle Scholar
  80. Rotini OT (1935) La trasformazione enzimatica dell’urea nel terreno. Ann Labor Ric Ferm Spallanrani 3:143–154Google Scholar
  81. Sarathchandra SU, Perrott KW (1981) Determination of phosphatase and arylsulphatase activity in soils. Soil Biol Biochem 13:543–545CrossRefGoogle Scholar
  82. Shapira R, Ordentlich A, Chet I, Oppenheim AB (1989) Control of plant diseases by chitinase expressed from cloned DNA in Escherichia coli. Phytopathology 79:1246–1249CrossRefGoogle Scholar
  83. Shawale JG, Sadana J (1981) Purification, characterization and properties of β-glucosidase enzyme from Sclerotium rolfsii. Arch Biochem Biophys 207:185–196CrossRefGoogle Scholar
  84. Simpson JR, Freney JR, Wetselaar R, Muirhead WA, Leuning R, Denmead OT (1984) Transformations and losses of urea nitrogen after application to flooded rice. Aust J Agric Res 35:189–200CrossRefGoogle Scholar
  85. Simpson JR, Freney JR (1988) Interacting processes in gaseous nitrogen loss from urea applied to flooded rice fields. In: Pushparajah E, Husin A, Bachik AT (eds) Proceedings of international symposium on urea technology and utilization. Malaysian Society of Soil Science, Kuala Lumpur, pp 281–290Google Scholar
  86. Singh PP, Shin YC, Park CS, Chung YR (1999) Biological control of Fusarium wilt of cucumber by chitinolytic bacteria. Phytopathology 89:92–99PubMedCrossRefGoogle Scholar
  87. Singer MJ, Ewing S (2000) Soil quality. In: Sumner ME (ed) Handbook of soil science. CRC, Boca Raton, FL, pp 271–298Google Scholar
  88. Sinsabaugh RL, Linkins AE (1989) Natural disturbance and the activity of Trichoderma viride cellulase complex. Soil Biol Biochem 21:835–839CrossRefGoogle Scholar
  89. Sinsabaugh RL, Antibus RK, Linkins AE (1991) An enzymic approach to the analysis of microbial activity during plant litter decomposition. Agric Ecosyst Environ 34:43–54CrossRefGoogle Scholar
  90. Speir TW, Ross DJ (1978) Soil phosphatase and sulphatase. In: Burns RG (ed) Soil enzymes. Academic, London, UK, pp 197–250Google Scholar
  91. Srinivasulu M, Rangaswamy V (2006) Activities of invertase and cellulase as influenced by the application of tridemorph and captan to groundnut (Arachis hypogaea) soil. Afr J Biotechnol 5:175–180Google Scholar
  92. Tabatabai MA (1977) Effect of trace elements on urease activity in soils. Soil Biol Biochem 9:9–13CrossRefGoogle Scholar
  93. Tabatabai MA (1982) Soil enzyme. In: Page AL (ed) Methods of soil analysis, Part 2. American Society of Agronomy, Madison, WI, pp 903–948Google Scholar
  94. Tabatabai MA (1994a) Soil enzymes. In: Weaver RW, Angle JS, Bottomley PS (eds) Methods of soil analysis, part 2. Microbiological and biochemical properties. SSSA Book Series No. 5. Soil Science Society of America, Madison, WI, pp 775–833Google Scholar
  95. Tabatabai MA (1994b) Soil enzymes. In: Mickelson SH (ed) Methods of soil analysis, Part 2. Microbiological and biochemical properties. Soil Science Society of America, Madison, WI, pp 775–833Google Scholar
  96. Tate RL (1995) Soil microbiology. John Wiley, New YorkGoogle Scholar
  97. Thoma JA, Spradlin JE, Dygert S (1971) Plant and animal amylases. In: Boyer PD (ed) The enzymes, 5th edn. Academic, New York, pp 115–189Google Scholar
  98. Torstensson L, Pell M, Stenberg B (1998) Need of a strategy for evaluation of arable soil quality. Ambio 27:4–8Google Scholar
  99. Trevors JT (1984) Dehydrogenase activity in soil: a comparison between the INT and TTC assay. Soil Biol Biochem 16:673–674CrossRefGoogle Scholar
  100. Tyler G (1981) Heavy metals in soil biology and biochemistry. In: Paul EA, Ladd JN (eds) Soil biochemistry, vol 5. Marcel Dekker, New York, pp 371–414Google Scholar
  101. Versaw WK, Harrison MJ (2002) A chloroplast phosphate transporter, PHT2; 1, influences allocation of phosphate within the plant and phosphate-starvation responses. Plant Cell 14:1751–1766PubMedCrossRefGoogle Scholar
  102. Vincent PG, Sisler HD (1968) Mechanisms of antifungal action of 2,4,5,6-tetrachloroisopathalonitrile. Physiol Plant 21:1249–1264CrossRefGoogle Scholar
  103. Vong PC, Dedourge O, Lasserre-Joulin F, Guckert A (2003) Immobilized-S, microbial biomass-S and soil arylsulphatase activity in the rhizosphere soil of rape and barley as affected by labile substrate C and N additions. Soil Biol Biochem 35:1651–1661CrossRefGoogle Scholar
  104. White AR (1982) Visualization of cellulases and cellulose degradation. In: Brown RM (ed) Cellulose and other natural polymer systems: biogenesis, structure, and degradation. Plenum, New York, pp 489–509CrossRefGoogle Scholar
  105. Wilke BM (1991) Effect of single and successive additions of cadmium, nickel and zinc on carbon dioxide evolution and dehydrogenase activity in a sandy Luvisol. Biol Fertil Soils 11:34–37CrossRefGoogle Scholar
  106. Yang Z, Liu S, Zheng D, Feng S (2006) Effects of cadmium, zinc and lead on soil enzyme activities. J Environ Sci 18:1135–1141CrossRefGoogle Scholar
  107. Zantua MI, Bremner JM (1977) Stability of urease in soils. Soil Biol Biochem 9:135–140CrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Department of Applied Chemistry and Biotechnology, Graduate School of EngineeringUniversity of FukuiFukuiJapan
  2. 2.Amity Institute of Microbial TechnologyAmity UniversityNoidaIndia

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