Skip to main content

Gene Function of Cucumber Mosaic Virus and its Satellite RNA Regarding Viral-host Interactions

  • Chapter
Experimental Plant Virology

Part of the book series: Advanced Topics in Science and Technology in China ((ATSTC,volume 0))

  • 880 Accesses

Abstract

Gene replacement using wild viruses as reference strains, the artificial reassortant of viral genome segments from different viral strains and utilization of artificial mutations, are principle methods to investigate the function of viral genes. In combination with viral diagnosis techniques, especially quantitative measuring, the relationship between the viral gene function and the pathogenicity of a virus could be demonstrated from the divergence of pathogenicity of these manipulated viruses. To set up a useful system for gene function study, the infectious clone is of preliminary importance. In fact, transgenic study and newly-developed siRNA methods for complementary suppression studies are also effective ways to understand the gene function of plant viruses.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ahlquist P, French R, Janda M, et al. (1984) Multicomponent RNA plant virus infection derived from cloned viral cDNA. Proc Natl Acad Sci USA 81(22): 7066–7070.

    Article  PubMed  CAS  Google Scholar 

  • Atreya CD and Pirone TP (1993) Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions and gene replacement on virulence and aphid transmissibility. Proc Natl Acad Sci USA 90(24): 11919–11923.

    Article  PubMed  CAS  Google Scholar 

  • Brigneti G, Voinnet O, Li WX, et al. (1998) Viral pathogenicity determinants are suppressers of transgene silencing in Nicotiana benthamiana. EMBO J 17: 6739–6746.

    Article  PubMed  CAS  Google Scholar 

  • Chapman EJ, Prokhnevsky AI, Gopinath K, et al. (2004) Viral RNA silencing suppressors inhibit the microRNA pathway at an intermediate step. Genes & Development 18(10): 1179–1186.

    Article  CAS  Google Scholar 

  • Collmer CW and Howell SH (1992) Role of satellite RNA in the expression of symptoms caused by plant viruses. Annu Rev Phytopathol 30: 419–442.

    Article  PubMed  CAS  Google Scholar 

  • Cronin S, Verchot J, Haldeman-Cahill R, et al. (1995) Long-distance movement factor: a transport function of the potyvirus helper component proteinase. Plant Cell 7(5): 549–559.

    Article  PubMed  CAS  Google Scholar 

  • Diaz-Pendon JA, Li F, Li WX, et al. (2007) Suppression of antiviral silencing by Cucumber mosaic virus 2b protein in Arabidopsis is associated with drastically reduced accumulation of three classes of viral small interfering RNAs. Plant Cell 19(6): 2053–2063.

    Article  PubMed  CAS  Google Scholar 

  • Ding SW, Anderson BJ, Haase HR and Symons RH (1994) New overlapping gene encoded by the Cucumber mosaic virus genome. Virology 198(2): 593–601.

    Article  PubMed  CAS  Google Scholar 

  • Ding SW, Li WX and Symons RH (1995) A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J 14(23): 5762–5772.

    PubMed  CAS  Google Scholar 

  • Ding SW, Shi BJ, Li WX, et al. (1996) An interspecies hybrid RNA virus is significantly more virulent than either parental virus. PNAS 93(15): 7470–7474.

    Article  PubMed  CAS  Google Scholar 

  • Diveki Z, Salanki K and Balazs E (2004) The necrotic pathotype of the Cucumber mosaic virus (CMV) ns strain is solely determined by amino acid 461 of the 1a protein. Mol Plant Microbe Interact 17(8): 837–845.

    Article  PubMed  CAS  Google Scholar 

  • Du ZY, Chen FF, Liao QS, et al. (2007) 2b ORFs encoded by subgroup IB strains of Cucumber mosaic virus induce differential virulence on Nicotiana species. J Gen Virol 88(Pt 9): 2596–2604.

    Article  PubMed  CAS  Google Scholar 

  • Feng JL, Chen SN, Tang XS, et al. (2006) Quantitative determination of Cucumber mosaic virus genome RNAs in virions by real-time reverse transcription-polymerase Chain reaction. Acta Biochim Biophys Sin 38(10): 669–676.

    Article  PubMed  CAS  Google Scholar 

  • Gal-On A, Canto T and Palukaitis P (2000) Characterisation of genetically modified Cucumber mosaic virus expressing histidine-tagged 1a and 2a proteins. Arch Virol 145(1): 37–50.

    Article  PubMed  CAS  Google Scholar 

  • Gal-On A, Kaplan I. and Palukaitis P (1995) Differential effects of satellite RNA on the accumulation of Cucumber mosaic virus RNAs and their encoded proteins in tobacco vs zucchini squash with two strains of CMV helper virus. Virology 208(1): 58–66.

    Article  PubMed  CAS  Google Scholar 

  • Gal-On A, Kaplan I, Roossinck MJ, et al. (1994) The kinetics of infection of zucchini squash by Cucumber mosaic virus indicate a function for RNA 1 in virus movement. Virology 205(1): 280–289.

    Article  PubMed  CAS  Google Scholar 

  • García-Arenal F and Palukaitis P (1999) Structure and functional relationships of satellite RNAs of Cucumber mosaic virus. In Satellites and Defective Viral RNAs (Vogt PK Jackson and AO, Eds.), Springer-Verlag, Berlin, pp: 37–63.

    Google Scholar 

  • Goto K, Kobori T, Kosaka Y, et al. (2007) Characterization of silencing suppressor 2b of Cucumber mosaic virus based on examination of its small RNA-binding abilities. Plant Cell Physiol 48(7): 1050–1060.

    Article  PubMed  CAS  Google Scholar 

  • Guo HS and Ding SW (2002) A viral protein inhibits the long range signaling activity of the gene silencing signal. EMBO J 21(3): 398–407.

    Article  PubMed  CAS  Google Scholar 

  • Higuchi R, Krummel B and Saiki RK (1988) A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res 16(15): 7351–7367.

    Article  PubMed  CAS  Google Scholar 

  • Ji LH and Ding SW (2001) The suppressor of transgene RNA silencing encoded by Cucumber mosaic virus interferes with salicylic acid-mediated virus resistance. Mol Plant Microbe Interact 14(6): 715–724.

    Article  PubMed  CAS  Google Scholar 

  • Kong Q, Oh JW and Simon AE (1995) Symptom attenuation by a normally virulent satellite RNA of turnip crinkle virus is associated with the coat protein open reading frame. Plant Cell 7(10): 1625–1634.

    Article  PubMed  CAS  Google Scholar 

  • Kong Q, Wang J and Simon AE (1997) Satellite RNA-mediated resistance to Turnip crinkle virus in Arabidopsis involves a reduction in virus movement. Plant Cell 9(11): 2051–2063.

    Article  PubMed  CAS  Google Scholar 

  • Lewsey M, Robertson FC, Canto T, et al. (2007) Selective targeting of miRNA-regulated plant development by a viral counter-silencing protein. Plant J 50(2): 240–252.

    Article  PubMed  CAS  Google Scholar 

  • Li F and Ding SW (2006) Virus counterdefense: diverse strategies for evading the RNA-silencing immunity. Annu Rev Microbiol 60: 503–531.

    Article  PubMed  CAS  Google Scholar 

  • Li HW, Lucy AP, Guo HS, Li WX, et al. (1999) Strong host resistance targeted against a viral suppressor of the plant gene silencing defence mechanism. EMBO J 18(10): 2683–2691.

    Article  PubMed  CAS  Google Scholar 

  • Lucy AP, Guo HS, Li WX, et al. (2000) Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus. EMBO J 19: 1672–1680.

    Article  PubMed  CAS  Google Scholar 

  • Masuta C and Takanami Y (1989) Determination of sequence and structural requirements for pathogenicity of a Cucumber mosaic virus satellite RNA (Y-satRNA). Plant Cell 1(12): 1165–1173.

    Article  PubMed  CAS  Google Scholar 

  • Mayers CN, Palukaitis P and Carr JP (2000) Subcellular distribution analysis of the Cucumber mosaic virus 2b protein. J Gen Virol 81(Pt 1): 219–226.

    PubMed  CAS  Google Scholar 

  • McGarvey P, Tousignant M, Geletka L, et al. (1995) The complete sequence of a Cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs. J Gen Virol 76 (Pt 9): 2257–2270.

    Article  PubMed  CAS  Google Scholar 

  • Moriones E, Diaz I, Rodriguez-Cerezo E, et al. (1992) Differential interactions among strains of Tomato aspermy virus and satellite RNAs of Cucumber mosaic virus. Virology 186(2): 475–480.

    Article  PubMed  CAS  Google Scholar 

  • Palukaitis P (1988) Pathogenicity regulation by satellite RNAs of Cucumber mosaic virus: minor nucleotide sequence changes alter host responses. Mol Plant Microbe Interact 1(4): 175–181.

    Article  PubMed  CAS  Google Scholar 

  • Palukaitis P and Garcia-Arenal F (2003) Cucumoviruses. Adv Virus Res 62: 241–323.

    Article  PubMed  CAS  Google Scholar 

  • Palukaitis P and Roossinck MJ (1996) Spontaneous change of a benign satellite RNA of Cucumber mosaic virus to a pathogenic variant. Nat Biotechnol 14(10): 1264–1268.

    Article  PubMed  CAS  Google Scholar 

  • Praveen S, Mangrauthia SK, Singh P, et al. (2008) Behavior of RNAi suppressor protein 2b of Cucumber mosaic virus in planta in presence and absence of virus. Virus Genes 37(1): 96–102.

    Article  PubMed  CAS  Google Scholar 

  • Rizzo TM and Palukaitis P (1990) Construction of full-length cDNA clones of Cucumber mosaic virus RNAs 1, 2 and 3: generation of infectious RNA transcripts. Mol Gen Genet 222(2–3): 249–256.

    Article  PubMed  CAS  Google Scholar 

  • Roossinck MJ and Palukaitis P (1991) Differential replication in zucchini squash of a Cucumber mosaic virus satellite RNA maps to RNA 1 of the helper virus. Virology 181(1): 371–373.

    Article  PubMed  CAS  Google Scholar 

  • Roossinck MJ, Sleat D and Palukaitis P (1992) Satellite RNAs of plant viruses: structures and biological effects. Microbiol Rev 56(2): 265–279.

    PubMed  CAS  Google Scholar 

  • Roth BM, Pruss GJ and Vance VB (2004) Plant viral suppressors of RNA silencing. Virus Res 102(1): 97–108.

    Article  PubMed  CAS  Google Scholar 

  • Sambrook J and Russell DW (2001) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

    Google Scholar 

  • Scholthof HB, Scholthof KB and Jackson AO (1995) Identification of Tomato bushy stunt virus host-specific symptom determinants by expression of individual genes from a potato virus X vector. Plant Cell 7(8): 1157–1172.

    Article  PubMed  CAS  Google Scholar 

  • Shi B, Miller J, Symons R, et al. (2003) The 2b protein of cucumoviruses has a role in promoting the cell-to-cell movement of pseudorecombinant viruses. Mol Plant Microbe Interact 16: 261–267.

    Article  PubMed  CAS  Google Scholar 

  • Shi BJ, Palukaitis P and Symons RH (2002) Differential virulence by strains of Cucumber mosaic virus is mediated by the 2b gene. Mol Plant Microbe Interact 15(9): 947–955.

    Article  PubMed  CAS  Google Scholar 

  • Shintaku MH, Zhang L and Palukaitis P (1992) A single amino acid substitution in the coat protein of Cucumber mosaic virus induces chlorosis in tobacco. Plant Cell 4(7): 751–757.

    Article  PubMed  CAS  Google Scholar 

  • Simon AE, Roossinck MJ and Havelda Z (2004) Plant virus satellite and defective interfering RNAs: new paradigms for a new century. Annu Rev Phytopathol 42: 415–437.

    Article  PubMed  CAS  Google Scholar 

  • Sleat DE and Palukaitis P (1992) A single nucleotide change within a plant virus satellite RNA alters the host specificity of disease induction. The Plant Journal 2(1): 43–49.

    Article  PubMed  CAS  Google Scholar 

  • Soards AJ, Murphy AM, Palukaitis P, et al. (2002) Virulence and differential local and systemic spread of Cucumber mosaic virus in tobacco are affected by the CMV 2b protein. Mol Plant Microbe Interact 15(7): 647–653.

    Article  PubMed  CAS  Google Scholar 

  • Soosaar JL, Burch-Smith TM and Dinesh-Kumar SP (2005) Mechanisms of plant resistance to viruses. Nat Rev Microbiol 3(10): 789–798.

    Article  PubMed  CAS  Google Scholar 

  • Suzuki M, Kuwata S, Masuta C, et al. (1995) Point mutations in the coat protein of Cucumber mosaic virus affect symptom expression and virion accumulation in tobacco. J Gen Virol 76 (Pt 7): 1791–1799.

    Article  PubMed  CAS  Google Scholar 

  • Szilassy D, Salanki K and Balazs E (1999) Stunting induced by Cucumber mosaic cucumovirus-infected Nicotiana glutinosa is determined by a single amino acid residue in the coat protein. Mol Plant Microbe Interact 12(12): 1105–1113.

    Article  PubMed  CAS  Google Scholar 

  • Voinnet O (2005) Induction and suppression of RNA silencing: insights from viral infections. Nat Rev Genet 6(3): 206–220.

    Article  PubMed  CAS  Google Scholar 

  • Wang Y, Gaba V, Yang J, et al. (2002) Characterization of synergy between Cucumber mosaic virus and Potyviruses in cucurbit hosts. Phytopathology 92(1): 51–58.

    Article  PubMed  Google Scholar 

  • Xin HW, Ji LH, Scott SW, et al. (1998) Ilarviruses encode a cucumovirus-like 2b gene that is absent in other genera within the bromoviridae. J Virol 72(8): 6956–6959.

    PubMed  CAS  Google Scholar 

  • Zhang X, Yuan YR, Pei Y, et al. (2006). Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes Dev 20(23): 3255–3268.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 2010 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Chen, J. (2010). Gene Function of Cucumber Mosaic Virus and its Satellite RNA Regarding Viral-host Interactions. In: Experimental Plant Virology. Advanced Topics in Science and Technology in China, vol 0. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-14119-5_4

Download citation

Publish with us

Policies and ethics