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HPTLC Hyphenated with FTIR: Principles, Instrumentation and Qualitative Analysis and Quantitation

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High-Performance Thin-Layer Chromatography (HPTLC)

Abstract

In recent years, much effort has been devoted to the coupling of high-performance thin-layer chromatography (HPTLC) with spectrometric methods because of the robustness and simplicity of HPTLC and the need for detection techniques that provide identification and determination of sample constituents. IR is one of the spectroscopic methods that have been coupled with HPTLC. IR spectroscopy has a high potential for the elucidation of molecular structures, and the characteristic absorption bands can be used for compound-specific detection. HPTLC–FTIR coupled method has been widely used in the modern laboratories for the qualitative and quantitative analysis. The potential of this method is demonstrated by its application in different fields of analysis such as drug analysis, forensic analysis, food analysis, environmental analysis, biological analysis, etc. The hyphenated HPTLC–FTIR technique will be developed in the future with the aim of taking full advantage of this method.

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References

  • Bauer GK and Kovar K-A. Direct HPTLC-FTIR On-line coupling: interaction of acids and bases with the binder in precoated HPTLC plates. J Planar Chromatogr-Mod TLC 1999; 11, 30–33.

    Google Scholar 

  • Bauer GK, Pfeifer AM, Hauck HE and Kovar K-A. Development of an optimized sorbent for direct HPTLC-FTIR on-line coupling. J Planar Chromatogr-Mod TLC 1998; 11, 84–89.

    CAS  Google Scholar 

  • Bayerbach S, Gauglitz G, Kovar K-A and Pisternick W (1999). Dünnschichtchromatographie in memoriam Professor Dr Jork, published by InCom Sonderband.

    Google Scholar 

  • Brandt C and Kovar K-A. Determination of 11-Nor-Δ9-tetrahydrocannabinol-9-carboxylic acid in urine by use of HPTLC-UV/FTIR on-line coupling. J Planar Chromatogr-Mod TLC 1997; 10, 348–352.

    CAS  Google Scholar 

  • Cimpoiu C, Miclaus V, Damian G, Puia M, Casoni D, Bele C and Hodisan T. Identification of new phtalazine derivatives by HPTLC-FTIR and characterization of their separation using some molecular properties. J Liq Chromatogr Relat Technol 2003; 26, 2687–2696.

    Article  CAS  Google Scholar 

  • Cseharti T and Forgacs E. Hyphenated techniques in thin layer chromatography. J Assoc Off Anal Chem 1998; 81, 329–332.

    Google Scholar 

  • Cserhati T, Forgacs E, Candeias M, Vilas-Boas L, Bronze R and Spranger I. Separation and tentative identification of the main pigment fraction of raisins by thin-layer chromatography – Fourier transform infrared and high performance liquid chromatography – ultraviolet detection. J Chromatogr Sci 2000; 38, 145–150.

    CAS  Google Scholar 

  • Cserhati T, Forgacs E, Morais MH and Ramos AC. TLC-FTIR of color pigments of chestnut sawdust. J Liq Chromatogr Relat Technol 2001; 24, 1435–1445.

    Article  CAS  Google Scholar 

  • Frey OR, Kovar K-A and Hoffmann V. Possibilities and limitations of assays by on-line coupling of thin layer chromatography and FTIR spectroscopy. J Planar Chromatogr-Mod TLC 1993; 6, 93–99.

    CAS  Google Scholar 

  • Glauninger G, Kovar K-A and Hoffmann V (1989). In Software Entwicklungen in der Chemie 3, Proceeding of 3rd Workshop Comput. Chem., Berlin, Germany, published by Springer, Berlin.

    Google Scholar 

  • Gocan S and Cimpan G. Compound identification in thin layer chromatography using spectrometric methods. Rev Anal Chem 1997; XVI, 1b–24b.

    Google Scholar 

  • Griffiths PR and De Haseth JA (1986). Fourier Transform Infrared Spectrometry, published by Wiley-Interscience, New York.

    Google Scholar 

  • Kovar K-A dan Hoffmann V. Möglichkeiten und Grenzen der direkten DC-FTIR Kopplung. GIT Spezial Chromatographie 1991; 11, 1197–1201.

    Google Scholar 

  • Kovar K-A, Enβlin HK, Frey OR, Rienas S and Wolff SC. Die direkte DC-IR-Kopplung zur Identifizierung und Bestimmung von Substanzgemischen. GIT Spezial Chromatographie 1991; 11, 95–101.

    CAS  Google Scholar 

  • Kovar K-A, Dinkelacker I, Pfeifer A, Pisternick W and Wössner A. Identifizierung von Suchstoffen mit Hilfe der HPTLC-UV/IR-Kopplung. GIT Spezial Chromatographie 1995; 15, 19–24.

    CAS  Google Scholar 

  • Morlock G and Kovar K-A (2003). Handbook of Thin Layer Chromatography, published by Marcel Dekker Inc., New York.

    Google Scholar 

  • Pfeifer AM and Kovar K-A. Identification of LSD, MBDB, and atropine in real samples with on-line HPTLC-FTIR coupling. J Planar Chromatogr-Mod TLC 1995; 8, 388–392.

    CAS  Google Scholar 

  • Pfeifer A, Tolimann G, Ammon HPT and Kovar K-A. Identification of adenosine in biological samples by HPTLC-FTIR on-line coupling. J Planar Chromatogr-Mod TLC 1996; 9, 31–34.

    CAS  Google Scholar 

  • Pisternick W, Kovar K-A and Ensslin H. High-performance thin-layer chromatogeaphic determination of N-ethyl-3,4-methylenedioxy-amphetamine and its major metabolites in urine and comparison with high performance liquid chromatography. J Chromatogr B 1997; 688, 63–69.

    Article  CAS  Google Scholar 

  • Rager IOC and Kovar K-A (2001). Planar Chromatography, published by Springer Scientific Publisher, Budapest.

    Google Scholar 

  • Somsen GW and Visser T (2003). Encyclopedia of Analytical Chemistry, published by John Wiley and Sons Ltd, Chichester.

    Google Scholar 

  • Somsen GW, Morden W and Wilson ID. Planar chromatography coupled with spectroscopic techniques. J Chromatogr 1995; 703, 613–665.

    Article  CAS  Google Scholar 

  • Stahlmann SA. Ten-year report on HPTLC-FTIR online coupling. J Planar Chromatogr-Mod TLC 1999; 12, 5–12.

    CAS  Google Scholar 

  • Stahlmann S and Kovar K-A. Analysis of impurities by high-performance thin-layer chromatography with Fourier transform infrared spectroscopy and UV absorbance detection in situ measurement: chlordiazepoxide in bulk powder and in tablets. J Chromatogr A 1998; 813, 145–152.

    Article  CAS  Google Scholar 

  • Wolff SC and Kovar K-A. Determination of edetic acid (EDTA) in water by on-line coupling of HPTLC and FTIR. J Planar Chromatogr-Mod TLC 1994; 7, 286–290.

    CAS  Google Scholar 

  • Wolff SC, Kovar K-A. Direct HPTLC-FTIR measurement in combination with AMD. J Planar Chromatogr-Mod TLC 1994; 7, 344–348.

    CAS  Google Scholar 

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Correspondence to Claudia Cimpoiu .

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Cimpoiu, C. (2011). HPTLC Hyphenated with FTIR: Principles, Instrumentation and Qualitative Analysis and Quantitation. In: Srivastava, M. (eds) High-Performance Thin-Layer Chromatography (HPTLC). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-14025-9_18

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