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Confocal Raman Microscopy pp 137–163Cite as

Raman Micro-spectral Imaging of Cells and Intracellular Drug Delivery Using Nanocarrier Systems

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Part of the book series: Springer Series in Optical Sciences ((SSOS,volume 158))

Abstract

Raman spectroscopy in combination with optical microscopy provides a new non-invasive method to examine and image cellular processes. Based on the spectral parameters of a cell’s components it is possible to image cellular organelles, such as the nucleus, chromatin, mitochondria, or lipid bodies, at the resolution of conventional microscopy. Several multivariate or spectral de-mixing algorithms, for example, hierarchical cluster analysis or orthogonal subspace projection, may be used to reconstruct an image of a cell. The non-invasive character of the technique as well as the associated chemical information may offer advantages over other imaging techniques such as fluorescence microscopy. Currently of particular interest are the uptake and intracellular fate of various pharmaceutical nanocarriers, which are widely used for drug delivery purposes, including intracellular drug and gene delivery. We have imaged the uptake and distribution patterns of several carrier systems over time. In order to distinguish the species of interest from their cellular environment spectroscopically, the carrier particles or the drug load itself may be labeled with deuterium. The first part of the chapter will briefly introduce the concept of Raman imaging in combination with multivariate data analysis on some simple cell models, after which the results of the uptake studies are discussed.

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References

  1. K. Schenke-Layland, I. Riemann, O. Damour, U. Stock, K. König, Adv. Drug Deliv. Rev. 58(7), 878 (2006)

    Article  Google Scholar 

  2. K. König, J. Microsc. 200(2), 83 (2000)

    Article  Google Scholar 

  3. A. García-Sáez, P. Schwille, Appl. Microbiol. Biotechnol. 76(2), 257 (2007)

    Article  Google Scholar 

  4. T. Kohl, P. Schwille, Adv. Biochem. Eng. Biotechnol. 95, 107 (2005)

    Google Scholar 

  5. N. Sijtsema, A. Tibbe, I. Segers-Nolten, A. Verhoeven, R. Weening, J. Greve, C. Otto, Biophys. J. 78(5), 2606 (2000)

    Article  Google Scholar 

  6. C. Otto, N. Sijtsema, J. Greve, Eur. Biophys. J. 27(6), 582 (1998)

    Article  Google Scholar 

  7. H.J. van Manen, Y. Kraan, D. Roos, C. Otto, Proc. Natl. Acad. Sci. USA 102(29), 10159 (2005)

    Article  Google Scholar 

  8. B. Wood, L. Hammer, L. Davis, D. McNaughton, J. Biomed. Opt. 10(1), 014005 (2005)

    Article  ADS  Google Scholar 

  9. S. Verrier, I. Notingher, J. Polak, L.L. Hench, Biopolymers 74(1–2), 157 (2004)

    Article  Google Scholar 

  10. K. Nithipatikom, M.J. McCoy, S. Hawi, K. Nakamoto, F. Adar, W. Campbell, Anal. Biochem. 322, 198 (2003)

    Article  Google Scholar 

  11. N. Uzunbajakava, A. Lenferink, Y. Kraan, E. Volokhina, G. Vrensen, J. Greve, C. Otto, Biophys. J. 84(6), 3968 (2003)

    Article  Google Scholar 

  12. N. Uzunbajakava, C. Otto, Opt. Lett. 2073–2075(28), 21 (2003)

    Google Scholar 

  13. C. Matthäus, S. Boydston-White, M. Miljković, M. Romeo, M. Diem, Appl. Spectrosc. 60(1), 1 (2006)

    Article  ADS  Google Scholar 

  14. N. Sijtsema, C. Otto, G. Segers-Nolten, A. Verhoeven, J. Greve, Biophys. J. 74(6), 3250 (1998)

    Article  ADS  Google Scholar 

  15. B. Wood, D. McNaughton, Expert Rev. Proteomics 3, 525 (2006)

    Article  Google Scholar 

  16. A. Feofanov, A. Grinchine, L. Shitova, T. Karmakova, R. Yakubovskaya, M. Egret-Charlier, P. Vigny, Biophys. J. 78(1), 499 (2000)

    Article  Google Scholar 

  17. S. Arzhantsev, A. Chikishev, N. Koroteev, J. Greeve, C. Otto, N. Sijtsema, J. Raman Spectrosc. 30, 205 (1999)

    Article  ADS  Google Scholar 

  18. C. Krafft, T. Knetschke, R. Funk, R. Salzer, Anal. Chem. 78(13), 4424 (2006)

    Article  Google Scholar 

  19. C. Matthäus, T. Chernenko, J. Newmark, C. Warner, M. Diem, Biophys. J. 93(2), 668 (2007)

    Article  ADS  Google Scholar 

  20. N. Keshava, Lincoln Lab. J. 14(1), 55 (2003)

    Google Scholar 

  21. J. Nascimento, J. Bioucas Dias, IEEE Trans. Geosci. Remote Sens. 43(4), 898 (2005)

    Article  ADS  Google Scholar 

  22. V. Torchilin, Annu. Rev. Biomed. Eng. 8, 343 (2006)

    Article  Google Scholar 

  23. C. Krafft, M. Kirsch, C. Beleites, G. Schackert, R. Salzer, Anal. Bioanal. Chem. 389(4), 1133 (2007)

    Article  Google Scholar 

  24. B. de Jong, T. Schut, K. Maquelin, T. van der Kwast, C. Bangma, D. Kok, G. Puppels, Anal. Chem. 78(22), 7761 (2006)

    Article  Google Scholar 

  25. C. Krishna, G. Sockalingum, R. Bhat, L. Venteo, P. Kushtagi, M. Pluot, M. Manfait, Anal. Bioanal. Chem. 387(5), 1649 (2007)

    Article  Google Scholar 

  26. C. Chang, IEEE Trans. Geosci. Remote Sens. 43(3), 502 (2005)

    Article  ADS  Google Scholar 

  27. S. Dipali, S. Kulkarni, G. Betageri, J. Pharm. Pharmacol. 48(11), 1112 (1996)

    Google Scholar 

  28. W. Liang, T. Levchenko, V. Torchilin, J. Microencapsul. 21, 151 (2004)

    Article  Google Scholar 

  29. V. Torchilin, T. Levchenko, A. Lukyanov, B. Kwah, A. Klibanov, R. Rammohan, G. Samokhin, K. Whiteman, Biochim. Biophys. Acta 1511, 397 (2001)

    Article  Google Scholar 

  30. D. Shenoy, M. Amiji, Int. J. Pharm. 293(1–2), 261 (2005)

    Article  Google Scholar 

  31. D. Naumann, Appl. Spectrosc. Rev. 36(2–3), 239 (2001)

    Article  ADS  Google Scholar 

  32. T. Spiro, Biological Applications of Raman Spectroscopy, vol. 1 (Wiley, 1987)

    Google Scholar 

  33. C. Yu, E. Gestl, K. Eckert, D. Allara, J. Irudayaraj, Cancer Detect. Prev. 30(6), 515 (2006)

    Article  Google Scholar 

  34. K. Short, S. Carpenter, J. Freyer, J. Mourant, Biophys. J. 88(6), 4274 (2005)

    Article  Google Scholar 

  35. T. Collins, M. Bootman, J. Exp. Biol. 206(12), 1993 (2003)

    Article  Google Scholar 

  36. C. Matthäus, T. Chernenko, L. Quintero, L. Milane, A. Kale, M. Amiji, V. Torchilin, M. Diem, Biophotonics: Photonic Solutions for Better Health Care, chap. Raman microscopic imaging of cells and applications monitoring the uptake of drug delivery systems (SPIE, Strasbourg, France, 2008).

    Google Scholar 

  37. J. van Blerkom, Reproduction 128, 269 (2004)

    Article  Google Scholar 

  38. C. Warner, J. Newmark, M. Comiskey, S. De Fazio, D. O’Malley, M. Rajadhyaksha, D. Townsend, S. McKnight, B. Roysam, P. Dwyer, C. DiMarzio, Reprod. Fertil. Dev. 16, 729 (2004)

    Article  Google Scholar 

  39. V. Torchilin, Eur. J. Pharm. Sci. 11(Supplement 2), S81 (2000)

    Article  Google Scholar 

  40. V. Torchilin, Adv. Drug Deliv. Rev. 57(1), 95 (2005)

    Article  Google Scholar 

  41. C. Plank, W. Zauner, E. Wagner, Adv. Drug Deliv. Rev. 34(1), 21 (1998)

    Article  Google Scholar 

  42. G. Kaul, M. Amiji, Pharm. Res. 22(6), 951 (2005)

    Article  Google Scholar 

  43. J. Panyam, V. Labhasetwar, Adv. Drug Deliv. Rev. 55(3), 329 (2003)

    Article  Google Scholar 

  44. J. Chawla, M. Amiji, Int. J. Pharm. 249(1–2), 127 (2002)

    Article  Google Scholar 

  45. V. Torchilin, Nat. Rev. Drug Discov. 4, 145 (2005)

    Article  Google Scholar 

  46. L. Serpe, M. Guido, R. Canaparo, E. Muntoni, R. Cavalli, P. Panzanelli, C. Della Pepal, A. Bargoni, A. Mauro, M. Gasco, M. Eandi, G. Zara, J. Nanosci. Nanotechnol. 6(9–10), 3062 (2006)

    Article  Google Scholar 

  47. N. Rao, V. Gopal, Biosci. Rep. 26(4), 301 (2006)

    Article  Google Scholar 

  48. U. Huth, R. Schubert, R. Peschka-Süss, J. Control. Release 110(3), 490 (2006)

    Article  Google Scholar 

  49. J. Wadia, R. Stan, S. Dowdy, Nat. Med. 10(3), 310 (2004)

    Article  Google Scholar 

  50. C. Matthäus, A. Kale, T. Chernenko, V. Torchilin, M. Diem, Mol. Pharm. 5(2), 287 (2008)

    Article  Google Scholar 

  51. J. Kristl, B. Volk, P. Ahlin, K. Gombac, M. Sentjurc, Int. J. Pharm. 256, 133 (2003)

    Article  Google Scholar 

  52. J. Wojewodzka, G. Pazdzior, M. Langner, Chem. Phys. Lipids 135(2), 181 (2005)

    Article  Google Scholar 

  53. C. Palmerini, C. Cametti, S. Sennato, D. Gaudino, E. Carlini, F. Bordi, G. Arienti, J. Membr. Biol. 211, 185 (2006)

    Article  Google Scholar 

  54. E. Kang, J. Robinson, K. Park, J. Cheng, J. Control. Release 122(3), 261 (2007)

    Article  Google Scholar 

  55. A. Belu, C. Mahoney, K. Wormuth, J. Control. Release 126(2), 111 (2008)

    Article  Google Scholar 

  56. D. Edwards, J. Hanes, G. Caponetti, J. Hrkach, A. Ben-Jebria, M. Eskew, J. Mintzes, D. Deaver, N. Lotan, R. Langer, Science 276(5320), 1868 (1997)

    Article  Google Scholar 

  57. L. Siskind, J. Bioenerg. Biomembr. 37(3), 143 (2005)

    Article  Google Scholar 

  58. A. Struckhoff, R. Bittman, M. Burow, S. Clejan, S. Elliott, T. Hammond, Y. Tang, B. Beckman, J. Pharmacol. Exp. Ther. 309(2), 523 (2004)

    Article  Google Scholar 

  59. E. Gulbins, H. Grassmé, Biochim. Biophys. Acta 1585(2–3), 139 (2002)

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, 02115, USA

    Christian Matthäus, Tatyana Chernenko, Miloš Miljković & Max Diem

  2. College of Engineering, University of Puerto Rico, Mayagües, PR, 00680, USA

    Luis Quintero

  3. Department of Pharmaceutical Sciences and Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, 02115, USA

    Lara Milane, Amit Kale, Mansoor Amiji & Vladimir Torchilin

Authors
  1. Christian Matthäus
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  2. Tatyana Chernenko
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  3. Luis Quintero
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  4. Miloš Miljković
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  5. Lara Milane
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  6. Amit Kale
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  7. Mansoor Amiji
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  8. Vladimir Torchilin
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  9. Max Diem
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Corresponding author

Correspondence to Christian Matthäus .

Editor information

Editors and Affiliations

  1. Instrumente und Technologie GmbH (WITec), Wissenschaftliche, Hoervelsinger Weg 6, Ulm, 89081, Germany

    Thomas Dieing

  2. Instrumente und Technologie GmbH (WITec), Wissenschaftliche, Hoervelsinger Weg 6, Ulm, 89081, Germany

    Olaf Hollricher

  3. Instrumente und Technologie GmbH (WITec), Wissenschaftliche, Hoervelsinger Weg 6, Ulm, 89081, Germany

    Jan Toporski

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Matthäus, C. et al. (2010). Raman Micro-spectral Imaging of Cells and Intracellular Drug Delivery Using Nanocarrier Systems. In: Dieing, T., Hollricher, O., Toporski, J. (eds) Confocal Raman Microscopy. Springer Series in Optical Sciences, vol 158. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-12522-5_7

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  • DOI: https://doi.org/10.1007/978-3-642-12522-5_7

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