High-Resolution Optical and Confocal Microscopy
In this chapter, the theory of optical image formation in an optical microscope is described, and the difference between conventional and confocal microscopy is explained. The selection of the appropriate pinhole diameter is discussed in detail, as the pinhole diameter is crucial in obtaining the highest depth resolution as well as optimizing collection efficiency, because the Raman signal is typically very weak.
KeywordsPoint Spread Func Image Formation Depth Resolution Total Energy Density Aperture Angle
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- 1.J.B. Pawley, Handbook of Biological Confocal Microscopy, 2nd edn. (Plenum Press, New York, 1995)Google Scholar
- 5.R. Juškaitis, T. Wilson, J. Microsc. 189, 8 (January 1998)Google Scholar
- 6.T. Wilson, Confocal Microscopy, Chap. IV.2. The imaging of simple objects (Academic, 1990) pp. 25–30Google Scholar
- 7.T. Wilson, Confocal Microscopy, Chap. IV.2. The imaging of simple objects (Academic, 1990) pp. 41–45Google Scholar
- 9.C.J.R. Sheppard, M. Gu, Optik 86(3), 104 (1990)Google Scholar