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High-Resolution Optical and Confocal Microscopy

  • Olaf HollricherEmail author
  • Wolfram Ibach
Chapter
Part of the Springer Series in Optical Sciences book series (SSOS, volume 158)

Abstract

In this chapter, the theory of optical image formation in an optical microscope is described, and the difference between conventional and confocal microscopy is explained. The selection of the appropriate pinhole diameter is discussed in detail, as the pinhole diameter is crucial in obtaining the highest depth resolution as well as optimizing collection efficiency, because the Raman signal is typically very weak.

Keywords

Point Spread Func Image Formation Depth Resolution Total Energy Density Aperture Angle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Research & DevelopmentWITec GmbHUlmGermany
  2. 2.WITec GmbHUlmGermany

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