Antimicrobial activity of Beta-Thujaplicin (Hinokitiol) on heterotrophic bacteria isolated from Reverse Osmosis water using for the preparation of Hemodialysis fluids

Abstract

To apply for the safety preparation of hemodialysis fluid, antimicrobial activities of beta-Thujaplicin (Hinokitiol) on heterotrophic bacteria such as Sphingomonas paucimobilis and Brevundimonas vesicularis which were isolated from Reverse Osmosis water using for the preparation of Hemodialysis fluids were examined. Less than 10 μg/ml (=ppm) of beta-Thujaplicin showed remarkable growth inhibition on Heterotrophic bacteria even they were incubated in optimal growth condition employing R2A liquid medium. This antimicrobial effect was more clearly observed when employed bacteria were incubated in RO water. Furthermore, this activity was not affected by heat treatment at 80°C for 10 min. These results suggested that small amount of beta-Thujaplicin cloud play useful supplementary substance in the hot water disinfection for several equipments of hemodialysis therapy.