Abstract
MLO proteins are structurally reminiscent of G-protein-coupled receptors but act independently of heterotrimeric G-proteins as major susceptibility factors to powdery mildew fungi. In barley, monomeric RAC/ROPs, instead of heterotrimeric G-proteins, MLO-dependently modulate susceptibility to powdery mildew, which may involve functions in cytoskeleton remodeling. In contrast to the role of RAC/ROPs in barley susceptibility to powdery mildew, rice OsRAC1 exerts a central function in basal and effector-triggered immunity. In this context, a functional cooperation with the heterotrimeric G-protein subunit, Gα, and additional protein complexes with functions in plant immunity has been discovered. These polypeptides together modulate the oxidative burst and regulate the abundance of defense-associated messenger RNAs and defense proteins. This chapter highlights the interconnection of MLO, RAC/ROP, and heterotrimeric G-proteins in plant immunity.
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Aepfelbacher M, Trasak C, Ruckdeschel K (2007) Effector functions of pathogenic Yersinia species. Thromb Haemost 98:521–529
Bai Y, Pavan S, Zheng Z, Zappel NF, Reinstädler A, Lotti C, De Giovanni C, Ricciardi L, Lindhout P, Visser R, Theres K, Panstruga R (2008) Naturally occurring broad-spectrum powdery mildew resistance in a central American tomato accession is caused by loss of Mlo function. Mol Plant Microbe Interact 21:30–39
Basu D, El-Din El-Assal D, Le J, Mallery EL, Szymanski DB (2004) Interchangeable functions of Arabidopsis PIROGI and the human WAVE complex subunit SRA1 during leaf epidermal development. Development 131:4345–4355
Basu D, Le J, Zakharova T, Mallery EL, Szymanski DB (2008) A SPIKE1 signaling complex controls actin-dependent cell morphogenesis through the heteromeric WAVE and ARP2/3 complexes. Proc Natl Acad Sci USA 105:4044–4049
Bedard K, Krause KH (2007) The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol Rev 87:245–313
Bednarek P, Pislewska-Bednarek M, Svatos A, Schneider B, Doubsky J, Mansurova M, Humphry M, Consonni C, Panstruga R, Sanchez-Vallet A, Molina A, Schulze-Lefert P (2009) A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense. Science 323:101–106
Beffa R, Szell M, Meuwly P, Pay A, Vögeli-Lange R, Métraux JP, Neuhaus G, Meins F, Nagy JR (1995) Cholera toxin elevates pathogen resistance and induces pathogenesis-related gene expression in tobacco. EMBO J 14:5753–5761
Bent AF, Mackey D (2007) Elicitors, effectors, and R genes: the new paradigm and a lifetime supply of questions. Ann Rev Phytopathol 45:399–436
Berken A, Wittinghofer A (2008) Structure and function of Rho-type molecular switches in plants. Plant Physiol Biochem 46:380–393
Bhat RA, Panstruga R (2005) Lipid rafts in plants. Planta 223:5–19
Block A, Li G, Fu ZQ, Alfano JR (2008) Phytopathogen type III effector weaponry and their plant targets. Curr Opin Plant Biol 11:396–403
Büschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A, van Daelen R, van der Lee T, Diergaarde P, Groenendijk J, Töpsch S, Vos P, Salamini F, Schulze-Lefert P (1997) The rarley Mlo gene: a novel control element of plant pathogen resistance. Cell 88:695–705
Chen CY, Cheung AY, Wu HM (2003) Actin-depolymerizing factor mediates Rac/Rop GTPase-regulated pollen tube growth. Plant Cell 15:237–49
Chen Y, Ji F, Xie H, Liang J, Zhang J (2006a) The regulator of G-protein signaling proteins involved in sugar and abscisic acid signaling in Arabidopsis seed germination. Plant Physiol 140:302–310
Chen Z, Hartmann HA, Wu MJ, Friedman EJ, Chen JG, Pulley M, Schulze-Lefert P, Panstruga R, Jones AM (2006b) Expression analysis of the AtMLO gene family encoding plant-specific seven-transmembrane domain proteins. Plant Mol Biol 60:583–597
Chisholm ST, Coaker G, Day B, Staskawicz BJ (2006) Host-microbe interactions: shaping the evolution of the plant immune response. Cell 124:803–814
Christensen TM, Vejlupkova Z, Sharma YK, Arthur KM, Spatafora JW, Albright CA, Meeley RB, Duvick JP, Quatrano RS, Fowler JE (2003) Conserved subgroups and developmental regulation in the monocot RAC/ROP gene family. Plant Physiol 133:1791–1808
Collins NC, Thordal-Christensen H, Lipka V, Bau S, Kombrink E, Qiu J-L, Hückelhoven R, Stein M, Freialdenhoven A, Somerville SC, Schulze-Lefert P (2003) SNARE-protein-mediated disease resistance at the plant cell wall. Nature 425:973–977
Consonni C, Humphry ME, Hartmann HA, Livaja M, Durner J, Westphal L, Vogel J, Lipka V, Kemmerling B, Schulze-Lefert P, Somerville SC, Panstruga R (2006) Conserved requirement for a plant host cell protein in powdery mildew pathogenesis. Nat Genet 38:716–720
da Cunha L, Sreerekha M-V, Mackey D (2007) Defense suppression by virulence effectors of bacterial phytopathogens. Curr Opin Plant Biol 10:349–357
Devoto A, Piffanelli P, Nilsson I, Wallin E, Panstruga R, von Heijne G, Schulze-Lefert P (1999) Topology, subcellular localization, and sequence diversity of the Mlo family in plants. J Biol Chem 274:34993–35004
Devoto A, Hartmann HA, Piffanelli P, Elliott C, Simmons C, Taramino G, Goh CS, Cohen FE, Emerson BC, Schulze-Lefert P (2003) Molecular phylogeny and evolution of the plant-specific seven-transmembrane MLO family. J Mol Evol 56:77–88
Ellis J, Catanzariti A-M, Dodds P (2006) The problem of how fungal and oomycete avirulence proteins enter plant cells. Trends Plant Sci 11:61–63
Felix G, Duran JD, Volko S, Boller T (1999) Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. Plant J 18:265–276
Flor HH (1942) Inheritance of pathogenicity in Melampsora lini. Phytopathol 32:653–669
Freialdenhoven A, Peterhansel C, Kurth J, Kreuzaler F, Schulze-Lefert P (1996) Identification of genes required for the function of non-race-specific mlo resistance to powdery mildew in barley. Plant Cell 8:5–14
Fu Y, Yang Z (2001) Rop GTPase: a master switch of cell polarity development in plants. Trends Plant Sci 6:545–547
Fu Y, Gu Y, Zheng Z, Wasteneys G, Yang Z (2005) Arabidopsis interdigitating cell growth requires two antagonistic pathways with opposing action on cell morphogenesis. Cell 120:687–700
Fujisawa Y, Kato T, Ohki S, Ishikawa A, Kitano H, Sasaki T, Asahi T, Iwasaki Y (1999) Suppression of the heterotrimeric G protein causes abnormal morphology, including dwarfism, in rice. Proc Natl Acad Sci USA 96:7575–7580
Fujiwara M, Umemura K, Kawasaki T, Shimamoto K (2006) Proteomics of Rac GTPase signalling reveals its predominant role in elicitor-induced defense response of cultured rice cells. Plant Physiol 140:734–745
Gao Y, Zeng Q, Guo J, Cheng J, Ellis BE, Chen JG (2007) Genetic characterization reveals no role for the reported ABA receptor, GCR2, in ABA control of seed germination and early seedling development in Arabidopsis. Plant J 52:1001–1013
Garcia-Brugger A, Lamotte O, Vandelle E, Bourque S, Lecourieux D, Poinssot B, Wendehenne D, Pugin A (2006) Early signaling events induced by elicitors of plant defenses. Mol Plant Microbe Interact 19:711–724
Gelli A, Higgins VJ, Blumwald E (1997) Activation of plant plasma membrane Ca2+-permeable channels by race-specific fungal elicitors. Plant Physiol 113:269–279
Gómez-Gómez L, Boller T (2000) FLS2: An LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis. Mol Cell 5:1003–1011
Gookin T, Kim J, Assmann S (2008) Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: computational prediction and in-vivo protein coupling. Genome Biol 9:R120
Guo J, Zeng Q, Emami M, Ellis BE, Chen J-G (2008) The GCR2 gene family is not required for ABA control of seed germination and early seedling development in Arabidopsis. PLoS ONE 3:e2982
Gruenheid S, Finlay BB (2003) Microbial pathogenesis and cytoskeletal function. Nature 422:775–781
Hassanain HH, Sharma YK, Moldovan L, Khramtsov V, Berliner LJ, Duvick JP, Goldschmidt-Clermont PJ (2000) Plant rac proteins induce superoxide production in mammalian cells. Biochem Biophys Res Commun 272:783–788
Hoefle C, Hückelhoven R (2008) Enemy at the gates – traffic at the plant cell pathogen interface. Cell Microbiol 10:2400–2407
Hückelhoven R (2007) Cell wall-associated mechanisms of disease resistance and susceptibility. Ann Rev Phytopathol 45:101–127
Hückelhoven R, Kogel K-H (2003) Reactive oxygen intermediates in plant-microbe interactions: who is who in powdery mildew resistance? Planta 216:891–902
Ishikawa A (2009) The Arabidopsis G-protein beta;-subunit is required for defense response against Agrobacterium tumefaciens. Biosci Biotech and Biochem 73:47–52
Jarosch B, Kogel K-H, Schaffrath U (1999) The ambivalence of the barley Mlo Locus: mutations conferring resistance against powdery mildew (Blumeria graminis f.sp. hordei) enhance susceptibility to the rice blast fungus Magnaporte grisea. Mol Plant Microbe Interact 12:508–514
Jeffrey CG, Daniel O, Wolf-Rüdiger S, Chenggang L, Mark S, Alan MJ (2008) d-Glucose sensing by a plasma membrane regulator of G signaling protein, AtRGS1. FEBS Lett 582:3577–3584
Johnston CA, Taylor JP, Gao Y, Kimple AJ, Grigston JC, Chen J-G, Siderovski DP, Jones AM, Willard FS (2007) GTPase acceleration as the rate-limiting step in Arabidopsis G protein-coupled sugar signaling. Proc Natl Acad Sci 104:17317–17322
Jones JDG, Dangl JL (2006) The plant immune system. Nature 444:323–329
Jørgensen IH (1992) Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley. Euphytica 63:141–152
Josefsson LG, Rask L (1997) Cloning of a putative G-protein-coupled receptor from Arabidopsis thaliana. Eur J Biochem 249:415–420
Kaku H, Nishizawa Y, Ishii-Minami N, Akimoto-Tomiyama C, Dohmae N, Takio K, Minami E, Shibuya N (2006) Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor. Proc Natl Aca Sci USA 103:11086–11091
Kao YY, Gianni D, Bohl B, Taylor RM, Bokoch GM (2008) Identification of a conserved Rac-binding site on NADPH oxidases supports a direct GTPase regulatory mechanism. J Biol Chem 283:12736–12746
Kawasaki T, Henmi K, Ono E, Hatakeyama S, Iwano M, Satoh H, Shimamoto K (1999) The small GTP-binding protein Rac is a regulator of cell death in plants. Proc Natl Acad Sci USA 96:10922–10926
Kawasaki T, Koita H, Nakatsubo T, Hasegawa K, Wakabayashi K, Takahashi H, Umemura K, Umezawa T, Shimamoto K (2006) Cinnamoyl-CoA reductase, a key enzyme in lignin biosynthesis, is an effector of small GTPase Rac in defense signaling in rice. Proc Natl Acad Sci USA 103:230–235
Kim J, Moriyama EN, Warr CG, Clyne PJ, Carlson JR (2000) Identification of novel multi-transmembrane proteins from genomic databases using quasi-periodic structural properties. Bioinformatics 16:767–775
Kim MC, Lee SH, Kim JK, Chun HJ, Choi MS, Chung WS, Moon BC, Kang CH, Park CY, Yoo JH, Kang YH, Koo SC, Koo YD, Jung JC, Kim ST, Schulze-Lefert P, Lee SY, Cho MJ (2002a) Mlo, a modulator of plant defense and cell death, is a novel calmodulin-binding protein. Isolation and characterization of a rice Mlo homologue. J Biol Chem 277:19304–19314
Kim MC, Panstruga R, Elliott C, Muller J, Devoto A, Yoon HW, Park HC, Cho MJ, Schulze-Lefert P (2002b) Calmodulin interacts with MLO protein to regulate defence against mildew in barley. Nature 416:447–451
Komatsu S, Yang G, Hayashi N, Kaku H, Umemura K, Iwasaki I (2004) Alterations by a defect in a rice G protein alpha subunit in probenazole and pathogen-induced responses. Plant Cell Environ 27:947–957
Kunze G, Zipfel C, Robatzek S, Niehaus K, Boller T, Felix G (2004) The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. Plant Cell 16:3496–3507
Kwon C, Neu C, Pajonk S, Yun HS, Lipka U, Humphry M, Bau S, Straus M, Kwaaitaal M, Rampelt H, Kasmi FE, Jurgens G, Parker J, Panstruga R, Lipka V, Schulze-Lefert P (2008) Co-option of a default secretory pathway for plant immune responses. Nature 451:835–840
Lavy M, Bracha-Drori K, Sternberg H, Yalovsky S (2002) A cell-specific, prenylation-independent mechanism regulates targeting of type II RACs. Plant Cell 14:2431–2450
Lavy M, Bloch D, Hazak O, Gutman I, Poraty L, Sorek N, Sternberg H, Yalovsky S (2007) A Novel RAC/ROP/RAC effector links cell polarity, root-meristem maintenance, and vesicle trafficking. Curr Biol 17:947–952
Legendre L, Heinstein PF, Low PS (1992) Evidence for participation of GTP-binding proteins in elicitation of the rapid oxidative burst in cultured soybean cells. J Biol Chem 267:20140–20147
Lieberherr D, Thao NP, Nakashima A, Umemura K, Kawasaki T, Shimamoto K (2005) A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice. Plant Physiol 138:1644–1652
Lipka V, Dittgen J, Bednarek P, Bhat R, Wiermer M, Stein M, Landtag J, Brandt W, Rosahl S, Scheel D, Llorente F, Molina A, Parker J, Somerville S, Schulze-Lefert P (2005) Pre- and postinvasion defenses both contribute to nonhost resistance in Arabidopsis. Science 310:1180–1183
Liu X, Yue Y, Li B, Nie Y, Li W, Wu W-H, Ma L (2007) A G protein-coupled receptor is a plasma membrane receptor for the plant hormone abscisic acid. Science 315:1712–1716
Llorente F, Alonso-Blanco C, Sánchez-Rodriquez C, Jorda L, Molina A (2005) ERECTA receptor-like kinase and heterotrimeric G protein from Arabidopsis are required for resistance to the necrotrophic fungus Plectosphaerella cucumerina. Plant J 43:165–180
Lyngkjaer MF, Newton AC, Atzema JL, Baker SJ (2000) The barley mlo-gene: an important powdery mildew resistance source. Agronomie 20:745–756
Miklis M, Consonni C, Bhat RA, Lipka V, Schulze-Lefert P, Panstruga R (2007) Barley MLO modulates actin-dependent and actin-independent antifungal defense pathways at the cell periphery. Plant Physiol 144:1132–1143
Miya A, Albert P, Shinya T, Desaki Y, Ichimura K, Shirasu K, Narusaka Y, Kawakami N, Kaku H, Shibuya N (2007) CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis. Proc Natl Aca Sci USA 104:19613–19618
Moeder W, Yoshioka K, Klessig DF (2005) Involvement of the small GTPase Rac in the defense responses of tobacco to pathogens. Mol Plant Microbe Interact 18:116–124
Molendijk AJ, Ruperti B, Singh MK, Dovzhenko A, Ditengou FA, Milia M, Westphal L, Rosahl S, Soellick TR, Uhrig J, Weingarten L, Huber M, Palme K (2008) A cysteine-rich receptor-like kinase NCRK and a pathogen-induced protein kinase RBK1 are RAC/ROP GTPase interactors. Plant J 53:909–923
Mongrand S, Morel J, Laroche J, Claverol S, Carde JP, Hartmann MA, Bonneu M, Simon-Plas F, Lessire R, Bessoule JJ (2004) Lipid rafts in higher plant cells: purification and characterization of Triton X-100-insoluble microdomains from tobacco plasma membrane. J Biol Chem 279:36277–36286
Morel J, Fromentin J, Blein JP, Simon-Plas F, Elmayan T (2004) Rac regulation of NtrbohD, the oxidase responsible for the oxidative burst in elicited tobacco cell. Plant J 37:282–293
Moriyama E, Strope P, Opiyo S, Chen Z, Jones A (2006) Mining the Arabidopsis thaliana genome for highly-divergent seven transmembrane receptors. Genome Biol 7:R96
Nakashima A, Chen L, Thao NP, Fujiwara M, Wong HL, Kuwano M, Umemura K, Shirasu K, Kawasaki T, Shimamoto K (2008) RACK1 functions in rice innate immunity by interacting with the Rac1 immune complex. Plant Cell 20:2265–2279
Ono E, Wong HL, Kawasaki T, Hasegawa M, Kodama O, Shimamoto K (2001) Essential role of the small GTPase Rac in disease resistance of rice. Proc Natl Acad Sci USA 98:759–764
Opalski KS, Schultheiss H, Kogel K-H, Hückelhoven R (2005) The receptor-like MLO protein and the RAC/ROP family G-protein HvRACB modulate actin reorganization in barley attacked by the biotrophic powdery mildew fungus Blumeria graminis f.sp. hordei. Plant J 41:291–303
Pandey S, Assmann SM (2004) The Arabidopsis putative G protein-coupled receptor GCR1 interacts with the G protein alpha-subunit GPA1 and regulates abscisic acid signaling. Plant Cell 16:1616–1632
Pandey S, Nelson DC, Assmann SM (2009) Two novel GPCR-type G proteins are abscisic acid receptors in Arabidopsis. Cell 136:136–148
Park J, Choi HJ, Lee S, Lee T, Yang Z, Lee Y (2000) Rac-related GTP-binding protein in elicitor-induced reactive oxygen generation by suspension-cultured soybean cells. Plant Physiol 124:725–732
Pathuri PI, Hensel G, Kumlehn J, Eichmann R, Hückelhoven R (2008) Constitutively activated barley ROPs modulate epidermal cell size, defense reactions and interactions with fungal leaf pathogens. Plant Cell Rep 27:1877–1887
Pathuri PI, Eichmann R, Hückelhoven R (2009) Plant small monomeric G-proteins (RAC/ROPs) of barley are common elements of susceptibility to fungal leaf pathogens, cell expansion and stomata development. Plant Signal Behav 4:109–110
Pease JE, Murphy PM (1998) Microbial corruption of the chemokine system: an expanding paradigm. Sem Immunol 10:169–178
Perfus-Barbeoch L, Jones AM, Assmann SM (2004) Plant heterotrimeric G protein function: insights from Arabidopsis and rice mutants. Curr Opin Plant Biol 7:719–731
Piffanelli P, Ramsay L, Waugh R, Benabdelmouna A, D'Hont A, Hollricher K, Jorgensen JH, Schulze-Lefert P, Panstruga R (2004) A barley cultivation-associated polymorphism conveys resistance to powdery mildew. Nature 430:887–891
Plakidou-Dymock S, Dymock D, Hooley R (1998) A higher plant seven-transmembrane receptor that influences sensitivity to cytokinins. Curr Biol 8:315–324
Ron M, Avni A (2004) The receptor for the fungal elicitor ethylene-inducing xylanase is a member of a resistance-like gene family in tomato. Plant Cell 16:1604–1615
Schaffrath U, Scheinpflug H, Reisener HJ (1995) An elicitor from Pyricularia oryzae induces resistance responses in rice: isolation, characterization and physiological properties. Physiol Mol Plant Pathol 46:293–307
Schiene K, Pühler A, Niehaus K (2000) Transgenic tobacco plants that express an antisense construct derived from a Medicago sativa cDNA encoding a Rac-related small GTP-binding protein fail to develop necrotic lesions upon elicitor infiltration. Mol Genet 263:761–770
Schultheiss H, Dechert C, Kogel K-H, Hückelhoven R (2002) A Small GTP-binding host protein is required for entry of powdery mildew fungus into epidermal cells of barley. Plant Physiol 128:1447–1454
Schultheiss H, Dechert C, Kogel K-H, Hückelhoven R (2003) Functional analysis of barley RAC/ROP G-protein family members in susceptibility to the powdery mildew fungus. Plant J 36:589–601
Schultheiss H, Hensel G, Imani J, Broeders S, Kumlehn J, Kogel K-H, Sonnewald U, Hückelhoven R (2005) Ectopic expression of constitutively activated HvRACB in barley enhances susceptibility to powdery mildew and abiotic stress. Plant Physiol 139:353–362
Schultheiss H, Preuss J, Pircher T, Eichmann R, Hückelhoven R (2008) Barley HvRIC171 interacts with HvRACB in planta and supports entry of the powdery mildew fungus. Cell Microbiol 10:1815–1826
Schwessinger B, Zipfel C (2008) News from the frontline: recent insights into PAMP-triggered immunity in plants. Curr Opin Plant Biol 11:389–395
Sorek N, Poraty L, Sternberg H, Bar E, Lewinsohn E, Yalovsky S (2007) Activation status-coupled transient S acylation determines membrane partitioning of a plant Rho-related GTPase. Mol Cell Biol 27:2144–2154
Stein M, Dittgen J, Sanchez-Rodriguez C, Hou B-H, Molina A, Schulze-Lefert P, Lipka V, Somerville S (2006) Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration. Plant Cell 18:731–746
Suharsono U, Fujisawa Y, Kawasaki T, Iwasaki Y, Satoh H, Shimamoto K (2002) The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice. Proc Natl Acad Sci USA 99:13307–13312
Temple BRS, Jones AM (2007) The plant heterotrimeric G-protein complex. Ann Rev Plant Biol 58:249–266
Thao NP, Chen L, Nakashima A, Hara S, Umemura K, Takahashi A, Shirasu K, Kawasaki T, Shimamoto K (2007) RAR1 and HSP90 form a complex with RAC/ROP GTPase and function in innate-immune responses in rice. Plant Cell 19:4035–4045
Trusov Y, Rookes JE, Chakravorty D, Armour D, Schenk PM, Botella JR (2006) Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling. Plant Physiol 140:210–220
Trusov Y, Rookes JE, Tilbrook K, Chakravorty D, Mason MG, Anderson D, Chen J-G, Jones AM, Botella JR (2007) Heterotrimeric G protein γ-subunits provide functional selectivity in Gβγ dimer signaling in Arabidopsis. Plant Cell 19:1235–1250
Tsukada K, Ishizaka M, Fujisawa Y, Iwasaki Y, Yamaguchi T, Minami E, Shibuya N (2002) Rice receptor for chitin oligosaccharide elicitor does not couple to heterotrimeric G-protein: elicitor responses of suspension cultured rice cells from Daikoku dwarf (d1) mutants lacking a functional G-protein alpha-subunit. Physiol Plant 116:373–382
Ullah H, Chen J-G, Temple B, Boyes DC, Alonso JM, Davis KR, Ecker JR, Jones AM (2003) The beta-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes. Plant Cell 15:393–409
Underwood W, Somerville SC (2008) Focal accumulation of defences at sites of fungal pathogen attack. J Exp Bot 59:3501–3508
Winge P, Brembu T, Kristensen R, Bones AM (2000) Genetic structure and evolution of RAC-GTPases in Arabidopsis thaliana. Genetics 156:1959–1971
Wong HL, Sakamoto T, Kawasaki T, Umemura K, Shimamoto K (2004) Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice. Plant Physiol 135:1447–1456
Wong HL, Pinontoan R, Hayashi K, Tabata R, Yaeno T, Hasegawa K, Kojima C, Yoshioka H, Iba K, Kawasaki T, Shimamoto K (2007) Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension. Plant Cell 19:4022–4034
Yalovsky S, Bloch D, Sorek N, Kost B (2008) Regulation of membrane trafficking, cytoskeleton dynamics, and cell polarity by ROP/RAC GTPases. Plant Physiol 147:1527–1543
Zhang W, He SY, Assmann SM (2008) The plant innate immunity response in stomatal guard cells invokes G-protein-dependent ion channel regulation. Plant J 56:984–996
Zipfel C (2008) Pattern-recognition receptors in plant innate immunity. Curr Opin Immunol 20:10–16
Zipfel C, Kunze G, Chinchilla D, Caniard A, Jones JDG, Boller T, Felix G (2006) Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation. Cell 125:749–760
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Lorek, J., Panstruga, R., Hückelhoven, R. (2010). The Role of Seven-Transmembrane Domain MLO Proteins, Heterotrimeric G-Proteins, and Monomeric RAC/ROPs in Plant Defense. In: Yalovsky, S., Baluška, F., Jones, A. (eds) Integrated G Proteins Signaling in Plants. Signaling and Communication in Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-03524-1_11
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