MHC: Peptide Analysis: Implications on the Immunogenicity of Hantaviruses’ N protein
Hantaviruses, members of the Bunyaviridae family, are enveloped negative-stranded RNA viruses with tripartite genomes – S, M and L. The S genome codes for a nucleocapsid (N) protein, which is quite conserved among different species of the hantavirus genus and possess a recognized immunogenicity. In this work we analyzed the sequence of two regions in this protein (N94 − 101 and N180 − 188), which presents T cell epitopes for two species of hantaviruses – Sin Nombre and Puumala. Interestingly, the same region has no described epitopes for Hantaan virus, despite its similarity. A study using a bioinformatic approach for the construction of MHC:peptide complexes was performed to detect any variation on the cleft region that could explain such degrees of immunogenicity. Our results shown topological and charges differences among the constructed complexes.
KeywordsMHC-I epitopes Hantavirus molecular docking
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