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Liquid chromatography of biomolecules

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Methods in Modern Biophysics
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Abstract

Proteins, peptides, DNA, RNA, lipids, and organic cofactors have various characteristics such as electric charge, molecular weight, hydrophobicity, and surface relief. Purification is usually achieved by using methods that separate the biomolecules according to their differences in these physical characteristics, such as ion exchange (Sect. 2.1), gel filtration (Sect. 2.2), and affinity chromatography (Sect. 2.3).

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Correspondence to Bengt Nölting .

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© 2009 Springer-Verlag Berlin Heidelberg

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Nölting, B. (2009). Liquid chromatography of biomolecules. In: Methods in Modern Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-03022-2_2

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  • DOI: https://doi.org/10.1007/978-3-642-03022-2_2

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-03021-5

  • Online ISBN: 978-3-642-03022-2

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