Abstract
Raman spectroscopy can be used to non-destructively add image contrast in visualizing structures and dynamics in living systems and materials. Image contrast can be derived from any information contained in Raman spectra, including band intensities, positions and widths. Because these parameters are functions of the local physical and chemical environment of a constituent, the images can display these properties as well. This chapter discusses instrumentation for acquiring low definition Raman maps and high definitions Raman images in two and three dimensions. Experimental configurations and their advantages and drawbacks are described. Methods for enhancing resolution are discussed. Finally several examples these techniques are presented, with an emphasis on application areas not elsewhere discussed in the book.
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Esmonde-White, F.W., Morris, M.D. (2010). Raman Imaging and Raman Mapping. In: Matousek, P., Morris, M. (eds) Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02649-2_5
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DOI: https://doi.org/10.1007/978-3-642-02649-2_5
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