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Applications of Raman and Surface-Enhanced Raman Scattering to the Analysis of Eukaryotic Samples

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Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

Abstract

In this chapter, we discuss Raman scattering and surface-enhanced Raman scattering (SERS) for the analysis of cellular samples of plant and animal origin which are several tens to hundreds of microns in size. As was shown in the past several years, the favorable properties of noble metal nanostructures can be used to generate SERS signals in very complex biological samples such as cells, and result in an improved sensitivity and spatial resolution. Pollen grains, the physiological containers that produce the male gametes of seed plants, consist of a few vegetative cells and one generative cell, surrounded by a biopolymer shell. Their chemical composition has been a subject of research of plant physiologists, biochemists [1, 2], and lately even materials scientists [3, 4] for various reasons. In spite of a multitude of applied analytical approaches it could not be elucidated in its entirety yet. Animal cells from cell cultures have been a subject of intense studies due to their application in virtually all fields of biomedical research, ranging from studies of basic biological mechanisms to models for pharmaceutical and diagnostic research. Many aspects of all kinds of cellular processes including signalling, transport, and gene regulation have been elucidated, but many more facts about cell biology will need to be understood in order to efficiently address issues such as cancer, viral infection or genetic disorder. Using the information from spectroscopic methods, in particular combining normal Raman spectroscopy and SERS may open up new perspectives on cellular biochemistry. New sensitive Raman–based tools are being developed for the biochemical analysis of cellular processes [5–8].

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Acknowledgments

We would like to thank the group of Dieter Naumann, Robert-Koch-Institut Berlin, for providing the cryostat and Peter Lasch, RKI and CytoSpec, Inc. for CytoSpec software. J.K. is grateful to Katrin Kneipp, Wellman Center for Photomedicine, Harvard Medical School, Boston, for providing Raman setups for SERS experiments in live cells. We gratefully acknowledge funding of this research by Deutsche Forschungsgemeinschaft (Grants DFG KN557/9-1 and PA 716/9-1).

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Authors and Affiliations

  1. Department of Chemistry, Humboldt University, Brook-Taylor-Str. 2, 12489, Berlin, Germany

    Franziska Schulte, Virginia Joseph, Ulrich Panne & Janina Kneipp

  2. BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, 12489, Berlin, Germany

    Franziska Schulte, Virginia Joseph, Ulrich Panne & Janina Kneipp

Authors
  1. Franziska Schulte
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  2. Virginia Joseph
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  3. Ulrich Panne
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  4. Janina Kneipp
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Correspondence to Franziska Schulte .

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Editors and Affiliations

  1. Science & Technology Facilities Council, Central Laser Facility, Didcot, Oxon., OX11 0QX, United Kingdom

    Pavel Matousek

  2. Dept. Chemistry, University of Michigan, Ann Arbor, 48109-1055, U.S.A.

    Michael D. Morris

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Schulte, F., Joseph, V., Panne, U., Kneipp, J. (2010). Applications of Raman and Surface-Enhanced Raman Scattering to the Analysis of Eukaryotic Samples. In: Matousek, P., Morris, M. (eds) Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02649-2_4

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  • DOI: https://doi.org/10.1007/978-3-642-02649-2_4

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