Abstract
The utility of confocal Raman microscopy to study biological events in skin is demonstrated with three examples. (i) monitoring the spatial and structural differences between native and cultured skin, (ii) tracking the permeation and biochemical transformation in skin of a Vitamin E derivative and (iii) tracking the spatial distribution of three major skin proteins (keratin, collagen, and elastin) during wound healing in an explant skin model.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
C. Xiao, D.J. Moore, M.E. Rerek, C.R. Flach, R. Mendelsohn, J. Invest. Dermatol. 124, 622 (2005)
G. Zhang, D.J. Moore, R. Mendelsohn, C.R. Flach, J. Invest. Dermatol. 126, 1088 (2006)
G. Zhang, D.J. Moore, K.B. Sloan, C.R. Flach, R. Mendelsohn, J. Invest. Dermatol. 127, 1205 (2007)
G. Zhang, D.J. Moore, C.R. Flach, R. Mendelsohn, Anal. Bioanal. Chem. 387, 1591 (2007)
G. Zhang, C.R. Flach, R. Mendelsohn, J. Control. Release 123, 141 (2007)
K.L.A. Chan, G. Zhang, M. Tomic-Canic, O. Stojadinovic, B. Lee, C.R. Flach, R. Mendelsohn, J. Cell. Mol. Med. 12, 2145 (2008)
P.M. Elias, J. Control Release 15, 199 (1991)
B. Forslind, Acta Derm. Venerol. 74, 1 (1994)
P.J. Caspers, G.W. Lucassen, R. Wolthuis, H.A. Bruining, G.J. Puppels, Biospectroscopy 4, S31 (1998)
P.J. Caspers, A.C. Williams, E.A. Carter, H.G. Edwards, B.W. Barry, H.A. Bruining, G.J. Puppels, Pharm. Res. 19, 1577 (2002)
P.J. Caspers, G.W. Lucassen, H.A. Bruining, G.J. Puppels, J. Raman Spectrosc. 31, 813 (2000)
P.J. Caspers, G.W. Lucassen, E.A. Carter, H.A. Bruining, G.J. Puppels, J. Invest. Dermatol. 116, 434 (2001)
P.D.A. Pudney, M. Mélot, P.J. Caspers, A. Van Der Pol, G.J. Puppels, Appl. Spectrosc. 61, 804 (2007)
A. Nijssen, T.C. Bakker Schut, F. Heule, P.J. Caspers, D.P. Hayes, M. Neumann, G.J. Puppels, J. Invest. Dermatol. 119, 64 (2002)
J. Wohlrab, A. Vollmann, S. Wartewig, W.C. Marsch, R. Neubert, Biopolymers 62, 141 (2001)
J. Choi, J. Choo, H. Chung, D.-G. Gweon, J. Park, H.J. Kim, S. Park, C.-H. Oh, Biopolymers 77, 264 (2005)
M. Gniadecka, P.A. Philipsen, S. Sigurdsson, S. Wessel, O.F. Nielsen, D.H. Christensen, J. Hercogova, K. Rossen, H.K. Thomsen, R. Gniadecki, L.K. Hansen, H.C. Wulf, J. Invest. Dermatol. 122, 443 (2004)
A. Nijssen, K. Maquelin, L.F. Santos, P.J. Caspers, T.C. Bakker Schut, J.C. den Hollander, M.H.A. Neumann, G.J. Puppels, J. Biomed. Opt. 12, 034004 (2007)
E. Ly, O. Piot, A. Durlach, P. Bernard, M. Manfait, Appl. Spectrosc. 62, 1088 (2008)
N.S. Eikje, K. Aizawa, Y. Ozaki, in Biotechnology Annual Review, vol. 11, ed. by M.R. El-Gewely (Elsevier, Amsterdam, 2005), p. 191
N.J. Everall, Appl. Spectrosc. 54, 1515 (2000)
N.J. Everall, Appl. Spectrosc. 54, 773 (2000)
N. Everall, Appl. Spectrosc. 62, 591 (2008)
T.E. Bridges, M.P. Houlne, J.M. Harris, Anal. Chem. 76, 576 (2004)
A.M. Macdonald, A.S. Vaughan, P.J. Wyeth, J. Raman Spectrosc. 36, 185 (2005)
B.W. Barry, H.G.M. Edwards, A.C. Williams, J. Raman Spectrosc. 23, 641 (1992)
J.L. Lippert, W.L. Peticolas, Proc. Natl. Acad. Sci. U S A 68, 1572 (1971)
J.J. Baraga, M.S. Feld, R.P. Rava, Proc. Natl. Acad. Sci. U S A 89, 3473 (1992)
R.D. Pensack, B.B. Michniak, D.J. Moore, R. Mendelsohn, Appl. Spectrosc. 60, 1399 (2006)
H. Deng, V.A. Bloomfield, J.M. Benevides, G.J. Thomas, Biopolymers 50, 656 (1999)
W.L. Peticolas, W.L. Kubasek, G.A. Thomas, M. Tsuboi, in Biological Applications of Raman Spectroscopy, vol. 1, ed. by T.G. Spiro (Wiley, New York, 1987), p. 81
R.C. Lord, G.J. Thomas, Spectrochim. Acta A 23, 2551 (1967)
A. Tfayli, O. Piot, F. Draux, F. Pitre, M. Manfait, Biopolymers 87, 261 (2007)
M.N. Siamwiza, R.C. Lord, M.C. Chen, T. Takamatsu, I. Harada, H. Matsuura, T. Shimanouchi, Biochemistry 14, 4870 (1975)
M. Rangarajan, J.L. Zatz, J. Cosmet. Sci. 52, 225 (2001)
W. Baschong, C. Artmann, D. Hueglin, J. Roeding, J. Cosmet. Sci. 52, 155 (2001)
A. Mavon, V. Raufast, D. Redoulés, J. Control. Release 100, 221 (2004)
G.C. Gurtner, S. Werner, Y. Barrandon, M.T. Longaker, Nature 453, 314 (2008)
P.A. Coulombe, J. Invest. Dermatol. 121, 219 (2003)
I.M. Freedberg, M. Tomic-Canic, M. Komine, M. Blumenberg, J. Invest. Dermatol. 116, 633 (2001)
A.J. Singer, R.A.F. Clark, N. Engl. J. Med. 341, 738 (1999)
M. Tomic-Canic, S.W. Mamber, O. Stojadinovic, B. Lee, N. Radoja, J. McMichael, Wound Repair Regen. 15, 71 (2007)
H. Brem, O. Stojadinovic, R.F. Diegelmann, H. Entero, B. Lee, I. Pastar, M. Golinko, H. Rosenberg, M. Tomic-Canic, Mol. Med. 13, 30 (2007)
B.G. Frushour, J.L. Koenig, Biopolymers 14, 379 (1975)
L. Debelle, A.J.P. Alix, M.-P. Jacob, J.-P. Huvenne, M. Berjot, B. Sombret, P. Legrand, J. Biol. Chem. 270, 26099 (1995)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Flach, C.R., Zhang, G., Mendelsohn, R. (2010). Raman Microscopy and Imaging: Applications to Skin Pharmacology and Wound Healing. In: Matousek, P., Morris, M. (eds) Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02649-2_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-02649-2_15
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-02648-5
Online ISBN: 978-3-642-02649-2
eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)