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Chemo-Mechanical Coupling in the Rotary Molecular Motor F1-ATPase

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Single Molecule Spectroscopy in Chemistry, Physics and Biology

Part of the book series: Springer Series in Chemical Physics ((CHEMICAL,volume 96))

Summary

F1-ATPase is a molecular motor in which the central γ subunit rotates inside the cylinder made of α3β3 subunits. The rotation is powered by ATP hydrolysis in three catalytic sites, and reverse rotation of the γ subunit by an external force leads to ATP synthesis in the catalytic sites. Single-molecule studies have revealed how the mechanical rotation is coupled to the chemical reactions in the three catalytic sites: binding/release of ATP, ADP, and phosphate, and hydrolysis/synthesis of ATP.

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Acknowledgements

We thank the members of Kinosita and Yoshida labs for collaboration and discussion, R. Kanda-Terada for technical support, K. Sakamaki, M. Fukatsu, and H. Umezawa for encouragement and lab management. This work was supported by Grants-in-Aids for Specially Promoted Research from the Ministry of Education, Sports, Culture, Science and Technology, Japan.

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Authors and Affiliations

  1. Department of Physics Faculty of Science and Engineering, Waseda University, Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan

    Kengo Adachi, Shou Furuike, Mohammad Delawar Hossain, Kazuhiko Kinosita Jr., Yasuhiro Onoue & Rieko Shimo-Kon

  2. Department of Physics, School of Physical Sciences Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh

    Mohammad Delawar Hossain

  3. Tsukuba Research Laboratory Hamamatsu Photonics KK Tokodai, Tsukuba, 300-2635, Japan

    Hiroyasu Itoh

  4. Department of Functional Molecular Science, The Graduate University for Advanced Studies (Sokendai), Okazaki, Aichi, 444-8585, Japan

    Yasuhiro Onoue

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  1. Kengo Adachi
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  2. Shou Furuike
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  3. Mohammad Delawar Hossain
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  4. Hiroyasu Itoh
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  5. Kazuhiko Kinosita Jr.
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Correspondence to Kazuhiko Kinosita Jr. .

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Editors and Affiliations

  1. Dept. Biophysics, Stockholm University, Stockholm, 106 91, Sweden

    Astrid Gräslund

  2. rue du Centre 115, St. Sulpice, 1025, Switzerland

    Rudolf Rigler

  3. Dept. Biomolecular Physics, Royal Institute of Technology (KTH), Stockholm, 106 91, Sweden

    Jerker Widengren

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Adachi, K. et al. (2010). Chemo-Mechanical Coupling in the Rotary Molecular Motor F1-ATPase. In: Gräslund, A., Rigler, R., Widengren, J. (eds) Single Molecule Spectroscopy in Chemistry, Physics and Biology. Springer Series in Chemical Physics, vol 96. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02597-6_14

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  • DOI: https://doi.org/10.1007/978-3-642-02597-6_14

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