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Force Measurements with Optical Tweezers

  • Othmar MartiEmail author
  • Katrin HübnerEmail author
Part of the Springer Handbooks book series (SHB)

Abstract

An optical tweezer is a scientific instrument that uses a focused laser beam to provide an attractive or repulsive force, depending on the index mismatch, to physically hold and move microscopic dielectric objects [33.1]:

Since their invention just over 20 years ago, optical traps have emerged as a powerful tool with broad-reaching applications in biology and physics. Capabilities have evolved from simple manipulation to the application of calibrated forces on – and the measurement of nanometer-level displacements of – optically trapped objects.

The ability to apply forces in the piconewton range to micrometer-sized particles while simultaneously measuring displacement with nanometer resolution is now routinely adopted for the study of molecular motors at the single-molecule level [33.2], the physics of colloids and mesoscopic systems [33.3,4], and the mechanical properties of polymers and biopolymers [33.5,6,7]. In parallel with the widespread use of optical trapping, theoretical and experimental work on fundamental aspects of optical trapping is being actively pursued [33.8,9,10]. In this chapter we will give a short overview of the principles of trapping and detection; different calibration methods, as well as the influence of surfaces and viscosity, will be discussed. The chapter ends with a short insight into the application of optical tweezers to cell biology.

Keywords

Corner Frequency Viscous Drag Gradient Force Optical Tweezer Trap Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

3-D

three-dimensional

ACF

autocorrelation function

CCD

charge-coupled device

F-actin

filamentous actin

IF

intermediate filament

IF

intermediate-frequency

MT

microtubule

NA

numerical aperture

OT

optical tweezers

PFM

photonic force microscope

PSD

position-sensitive detector

PSD

position-sensitive diode

PSD

power-spectral density

QPD

quadrant photodiode

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Institute of Experimental PhysicsUlm UniversityUlmGermany
  2. 2.Staatliche Fachoberschule Neu-UlmNeu-UlmGermany

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