Skip to main content

Comparative In Silico Evaluation of MYB Transcription Factors in Eucalyptus, Sugarcane and Rice Transcriptomes

  • Conference paper
Computational Intelligence Methods for Bioinformatics and Biostatistics (CIBB 2008)

Abstract

Transcription factors are essential in plant growth and development regulation and during response activation to environmental stimuli. This study examined 237,954 sugarcane and 110,000 eucalyptus ESTs in search of genes related to MYB factors active during abiotic stresses in arabidopsis and rice. Searches revealed relative abundance and diversity of MYB candidate sequences in both transcriptomes. In general, 71 and 42 MYB sequences have been selected in eucalyptus and sugarcane, respectively. The generated phenogram, using selected sequences and sequences from other organisms, revealed a sub-grouping according to domain-repeats number; confirming the ancestral origin of MYB genes. Expression pattern analysis revealed higher number of reads in wood, flower and bark libraries in eucalyptus, and in tissues infected by pathogens in sugarcane. Splicing pattern comparative analyses of arabidopsis and rice ORFs with eucalyptus and sugarcane ORFs revealed that neither of them presented similarities regarding all exons and intron junctions.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Munns, R.: Comparative Physiology of Salt and Water Stress. Plant Cell Environ. 28, 239–250 (2002)

    Article  Google Scholar 

  2. Wang, W., Vinocur, B., Altman, A.: Plant Responses to Drought, Salinity and Extreme Temperatures: Towards Genetic Engineering for Stress Tolerance. Planta 218, 1–14 (2003)

    Article  CAS  PubMed  Google Scholar 

  3. Riechmann, J.L., Herad, J., Martin, G., Reuber, L., Jiang, C.-Z., Keddie, J., Adam, L., Pineda, O., Ratcliffe, O.J., Samaha, R.R., Creelman, R., Pilgrim, M., Broun, P., Zhang, J.Z., Ghandehari, D., Sherman, B.K., Yu, G.: Arabidopsis Transcription Factors: Genome-Wide Comparative Analysis among Eukaryotes. Science 290, 2105–2110 (2000)

    Article  CAS  PubMed  Google Scholar 

  4. Liu, L., White, M.J., MacRae, T.H.: Transcription Factors and their Genes in Higher Plants: Functional Domains Evolution and Regulation. Eur. J. Biochem. 262, 247–257 (1999)

    Article  CAS  PubMed  Google Scholar 

  5. Jin, H., Martin, C.: Multifunctionality and Diversity within the Plant MYB-Gene Family. Plant Mol. Biol. 41, 577–585 (1999)

    Article  CAS  PubMed  Google Scholar 

  6. Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., Satou, M., Akiyama, K., Taji, T., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y., Shinozaki, K.: Monitoring the Expression Profiles of 7,000 Arabidopsis Genes under Drought Cold and High-Salinity Stresses Using a Full-Length cDNA Microarray. Plant J. 31, 279–292 (2002)

    Article  CAS  PubMed  Google Scholar 

  7. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J.: Basic Local Alignment Search Tool. J. Mol. Biol. 251, 403–410 (1990)

    Article  Google Scholar 

  8. Sneath, P.H.A., Sokal, R.R.: Numerical Taxonomy. Freeman, San Francisco (1973)

    Google Scholar 

  9. Page, R.D.: Treeview Program Version 161. Comput. Appl. Biosci. 12, 357–358 (1996)

    CAS  PubMed  Google Scholar 

  10. Eisen, M.B., Spellman, P.T., Brown, P.O., Botstein, D.: Cluster Analysis and Display of Genomic-Wide Expression Pattern. Proc. Natl. Acad. Sci. USA 95, 14863–14868 (1998)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Hiller, K., Schobert, M., Hundertmark, C., Jahn, D., Munch, R.: JVirGel: Calculation of Virtual Two-Dimensional Protein Gels. Nucleic Acids Res. 31, 3862–3865 (2003)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Papini-Terzi, F.S., Rocha, F.R., Vêncio, R.Z.N., Oliveira, K.C., Felix, J.M., Vicentini, R., Rocha, C.S., Simões, A.C.Q., Ulian, E.C., Mauro, S.M.Z., Silva, A.M., Pereira, C.A.B., Menossi, M., Souza, G.M.: Transcription Profiling of Signal Transduction-Related Genes in Sugarcane Tissues. DNA Res. 12, 27–38 (2005)

    Article  CAS  PubMed  Google Scholar 

  13. Yanhui, C., Xiaoyuan, Y., Kun, H., Meihua, L., Jigang, L., Zhaofeng, G., Zhiqiang, L.: The MYB Transcription Factor Superfamily of Arabidopsis: Expression Analysis and Phylogenetic Comparison with the Rice MYB Family. Plant Mol. Biol. 60, 107–124 (2006)

    Article  PubMed  Google Scholar 

  14. Rabbani, M.A., Maruyama, K., Abe, H., Khan, M.A., Katsura, K., Ito, Y., Yoshiwara, K., Seki, M., Shinozaki, K., Yamaguchi-Shinozaki, K.: Monitoring Expression Profiles of Rice Genes under Cold, Drought, and High-Salinity Stresses and Abscisic Acid Application Using cDNA Microarray and RNA Gel-Blot Analyses. Plant Physiol. 133, 1755–1767 (2003)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Thompson, M.A., Ramsay, R.G.: Myb: an Old Oncoprotein with New Roles. Bioessays 17, 341–350 (1995)

    Article  CAS  PubMed  Google Scholar 

  16. Lipsick, J.S.: One Billion Years of MYB. Oncogene 13, 223–235 (1996)

    CAS  PubMed  Google Scholar 

  17. Kamiya, T., Kawabe, A., Miyashita, N.T.: Nucleotide Polymorphism at Atmyb2 Locus of Wild Plant Arabidopsis thaliana. Genet. Res. 80, 89–98 (2002)

    Article  CAS  PubMed  Google Scholar 

  18. Shikata, M., Matsuda, Y., Ando, K., Nishii, A., Takemura, M., Yokota, A., Kohchi, T.: Characterization of Arabidopsis ZIM a Member of Novel Plant-Specific GATA Factor Gene Family. J. Exp. Bot. 55, 631–639 (2004)

    Article  CAS  PubMed  Google Scholar 

  19. Park, J.M., Park, C.J., Lee, S.B., Ham, B.K., Shin, R., Paek, K.H.: Overexpression of the Tobacco Tsi1 Gene Encoding an EREBP/AP2-Type Transcription Factor Enhances Resistance Against Pathogen Attack and Osmotic Stress in Tobaccoy. Plant Cell 13, 1035–1046 (2001)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Wu, K.-L., Guo, Z.-J., Wang, H.-H., Li, J.: The WRKY Family of Transcription Factors in Rice and Arabidopsis and their Origins. DNA Res. 12, 9–26 (2005)

    Article  CAS  PubMed  Google Scholar 

  21. Reyes, J.C., Muro-Pastor, M.I., Florêncio, F.J.: The GATA Family of Transcription Factors in Arabidopsis and Rice. Plant Physiol. 134, 1718–1732 (2004)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Roy, S.W., Penny, D.: Patterns of Introns Loss and Gain in Plants: Intron Loss-Dominated Evolution and Genome-Wide Comparison of O. sativa and A. thaliana. Mol. Biol. Evol. 24, 171–181 (2007)

    Article  CAS  PubMed  Google Scholar 

  23. Jiang, C., Gu, X., Peterson, T.: Identification of Conserved Gene Structure and Carboxy-Terminal Motifs in the MYB Gene Family of Arabidopsis and Oryza sativa L. ssp. indica. Genome Biol. 5, 1–11 (2004)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Soares-Cavalcanti, N.M., Wanderley-Nogueira, A.C., Belarmino, L.C., dos Santos Barros, P., Benko-Iseppon, A.M. (2009). Comparative In Silico Evaluation of MYB Transcription Factors in Eucalyptus, Sugarcane and Rice Transcriptomes. In: Masulli, F., Tagliaferri, R., Verkhivker, G.M. (eds) Computational Intelligence Methods for Bioinformatics and Biostatistics. CIBB 2008. Lecture Notes in Computer Science(), vol 5488. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02504-4_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-02504-4_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-02503-7

  • Online ISBN: 978-3-642-02504-4

  • eBook Packages: Computer ScienceComputer Science (R0)

Publish with us

Policies and ethics