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Initial Events Associated with Virus PBCV-1 Infection of Chlorella NC64A

Chapter
First Online:
Part of the Progress in Botany book series (BOTANY, volume 71)

Abstract

Chlorella viruses (or chloroviruses) are very large, plaque-forming viruses. The viruses are multilayered structures containing a large double-stranded DNA genome, a lipid bilayered membrane, and an outer icosahedral capsid shell. The viruses replicate in certain isolates of the coccal green alga, Chlorella. Sequence analysis of the 330-kbp genome of Paramecium bursaria Chlorella virus 1 (PBCV-1), the prototype of the virus family Phycodnaviridae, reveals <365 protein-encoding genes and 11 tRNA genes. Products of about 40% of these genes resemble proteins of known function, including many that are unexpected for a virus. Among these is a virus-encoded protein, called Kcv, which forms a functional K+ channel. This chapter focuses on the initial steps in virus infection and provides a plausible role for the function of the viral K+ channel in lowering the turgor pressure of the host. This step appears to be a prerequisite for delivery of the viral genome into the host.

Keywords

Host Cell Turgor Pressure Membrane Voltage Freshwater Alga Fusion Pore 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

The authors are grateful for financial support for this research by the Deutsche Forschungsgemeinschaft to GT and the European Drug Initiative on Channels and Transporters (EDICT) project EU FP7 (201924) to A.M. Research in the Van Etten laboratory was supported in part by Public Health Service grant GM32441 from the National Institute of General Medical Sciences and NIH grant P20-RR1565 from the COBRE program of the National Center for Research Resources.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Institute of BotanyTechnische Universität DarmstadtDarmstadtGermany
  2. 2.Dipartimento di BiologiaUniversità degli Studi di Milano, E Istituto di Biofisica, CNR, U-MilanoMilanoItaly
  3. 3.Department of Plant Pathology and Nebraska Center for VirologyUniversity of NebraskaLincolnUSA

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