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Biocompatible Visualization of Flow Fields Generated by Microorganisms

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Part of the book series: Notes on Numerical Fluid Mechanics and Multidisciplinary Design ((NNFM,volume 106))

Abstract

Microflow induced by the ciliates called Opercularia asymmetrica is an interesting phenomenon in biofluidmechanics. Ciliates play an important role in the structural formation of microbial granules derived from activated sludge. Additionally, flow induced by protozoa (ciliates) is treated as an efficient mean of nutrient transport with minimum energy requirement. For the first time powerful digital imaging techniques are used for studying microorganismic convection. Investigations of the flow generated by Opercularia asymmetrica are carried out with help of digital micro Particle Image Velocimetry. Digital micro Particle Tracking Velocimetry is implemented to analyse cilia motion. In biological fluid mechanics flow visualization techniques must guarantee biocompatibility. Thus, in the present work appropriate light illumination and suitable seeding particles are used. Moreover, in order to predict artefacts and correct them novel neuronumerical hybrid is employed.

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References

  1. Benning, R., Becker, T., Delgado, A.: Initial studies of predicting flow fields with an ANN hybrid. Adv. Eng. Softw. 32, 895–901 (2001)

    Article  MATH  Google Scholar 

  2. Delgado, A., Nirchl, H., Becker, T.: First use of cognitive algorithms in investigations under compensated gravity. Micrograv. Sci. Technol. IX (3), 185–192 (1996)

    Google Scholar 

  3. Delgado, A., Petermeier, H., Kowalczyk, W.: Micro-PIV in Life Science, Particle Image Velocimetry. In: Raffel, M., Willert, C., Wereley, S., Kompenhans, J. (eds.), 2nd edn. Springer, Heidelberg (2007)

    Google Scholar 

  4. Díez, L., Zima, B.E., Kowalczyk, W., Delgado, A.: Investigation of multiphase flow in Sequencing Batch Reactor (SBR) by means of hybrid methods. Chem. Eng. Sci. 67, 1803–1813 (2006)

    Google Scholar 

  5. Etterer, T., Wilderer, P.A.: Generation and properties of aerobic granular sludge. Water Sci. Technol. 43, 19–26 (2001)

    Google Scholar 

  6. Hartmann, C., Özmutlu, Ö., Petermeier, H., Fried, J., Delgado, A.: Analysis of the flow field induced by the sessile peritrichous ciliate Opercularia asymmetrica. J. Biomech. 40, 137–148 (2007)

    Article  Google Scholar 

  7. Kowalczyk, W., Zima, B.E., Delgado, A.: A biocompatible seeding particle approach for μ-PIV measurements of a fluid flow provoked by microorganisms. Exp. Fluids 43, 147–150 (2007)

    Article  Google Scholar 

  8. Lindken, R., Gui, L., Merzkirch, W.: Velocity measurements in multiphase flow by means of Particle Image Velocimetry. Chem. Eng. Technol. 22, 202–206 (1999)

    Article  Google Scholar 

  9. Petermeier, H., Benning, R., Delgado, A., Kulozik, U., Hinrichs, J., Becker, T.: Hybrid model of the fouling process in tubular heat exchangers for the dairy industry. J. Food Eng. 55, 9–17 (2002)

    Article  Google Scholar 

  10. Petermeier, H., Kowalczyk, W., Delgado, A., Denz, C., Holtmann, F.: Detection of microorganismic flows by linear and nonlinear optical methods and automatic correction of erroneous images artefacts and moving boundaries in image generating methods by a neuronumerical hybrid implementing the Taylor’s hypothesis as a priori knowledge. Exp. Fluids 42, 611–623 (2007)

    Article  Google Scholar 

  11. Quenot, G.M., Pakleza, J., Kowalewski, T.A.: Particle Image Velocimetry with optical flow. Exp. Fluids 25, 177–189 (1998)

    Article  Google Scholar 

  12. Raffel, M., Willert, C.E., Kompenhans, J.: Particle Image Velocimetry. A Practical Guide. Springer, Heidelberg (1998)

    Google Scholar 

  13. Sleigh, M.A., Barlow, D.: Collection of food by Vorticella. Trans. Am. Microsc. Soc. 95, 482–486 (1976)

    Article  Google Scholar 

  14. Tay, J., Liu, Q., Liu, Y.: The role of cellular polysaccharides in the formation and stability of aerobic granules. Lett. Appl. Microbiol. 33, 222–226 (2001)

    Article  Google Scholar 

  15. Vopel, K., Reick, C.H., Arlt, G., Pöhn, M., Ott, J.A.: Flow microenvironment of two marine ciliates with ectobiotic chemoautotrophic bacteria. Aquat. Microb. Ecol. 29, 19–28 (2002)

    Article  Google Scholar 

  16. Weber, S., Ludwig, W., Schleifer, K.H., Fried, J.: Microbial composition and structure of aerobic granular sewage biofilms. Appl. Environ. Microbiol. 73, 6233–6240 (2007)

    Article  Google Scholar 

  17. Westerweel, J., Dabiri, D., Gharib, M.: The effect of a discrete window offset on the accuracy of cross–correlation analysis of digital particle image velocimetry. Exp. Fluids 23, 20–28 (1997)

    Article  Google Scholar 

  18. Willert, C., Gharib, M.: Digital Particle Image Velocimetry. Exp. Fluids 10, 181–193 (1991)

    Article  Google Scholar 

  19. Zima, B.E., Diez, L., Kowalczyk, W., Delgado, A.: Sequencing Batch Reactor (SBR) as optimal method for production of Granular Activated Sludge (GAS) – fluid dynamic investigations. Water Sci. Technol. 55, 151–158 (2007)

    Google Scholar 

  20. Zima-Kulisiewicz, B.E., Díez, L., Kowalczyk, W., Hartmann, C., Delgado, A.: Biofluid mechanical investigations in Sequencing Batch Reactor (SBR). Chem. Eng. Sci. 63, 599–608 (2008)

    Article  Google Scholar 

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Zima-Kulisiewicz, B.E., Botello-Payro, E., Delgado, A. (2009). Biocompatible Visualization of Flow Fields Generated by Microorganisms. In: Nitsche, W., Dobriloff, C. (eds) Imaging Measurement Methods for Flow Analysis. Notes on Numerical Fluid Mechanics and Multidisciplinary Design, vol 106. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01106-1_27

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  • DOI: https://doi.org/10.1007/978-3-642-01106-1_27

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-01105-4

  • Online ISBN: 978-3-642-01106-1

  • eBook Packages: EngineeringEngineering (R0)

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