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Electrophoresis of Dyes and Proteins in Poly(Acrylamide) Gel Containing Immobilized Bilayer Membranes

  • Conference paper
Book cover Gels: Structures, Properties, and Functions

Part of the book series: Progress in Colloid and Polymer Science ((PROGCOLLOID,volume 136))

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Abstract

Electrophoresis of dye stuffs and proteins in poly(acrylamide) gel containing immobilized bilayer membranes have been studied. Bilayer membranes of a polymerizable surfactant, dodecylglyceryl itaconate (DGI), can be immobilized in poly(acrylamide) gels, and the hybrid gels are first applied to a substrate of the poly(acrylamide) gel electrophoresis (PAGE). The bilayer-membranes-immobilized-gel (abbreviated as BM-gel) showed different separation behaviors from those by the conventional PAGE. The separation behavior of dye stuffs suggests that the bilayer membranes in the BM-gel work as a separator of the test molecules due to their hydrophilic/hydrophobic nature. Water-soluble proteins migrated faster in the BM-gels than in the simple poly(acrylamide) gels. Membrane proteins, on the other hand, did not move at all in the BM-gels probably because the protein molecules were entrapped firmly inside the bilayer membranes.

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Acknowlegdements

The authors thank the financial support of the Grant-in-Aid for Scientific Research (No. 17655071) from Ministry of Education, Culture, Sports, Science, and Technology, Japan. G. M. acknowledges also to Shimadzu Science Foundation, and Shiseido Science Foundation, Japan for their financial support. We also appreciate Professor Naoki Kamo of Hokkaido University for his kind gift of the sample of bacteriorhodopsin.

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Authors and Affiliations

  1. Nanotechnology Research Center, Research Institute for Electronic Science, Hokkaido University, (CRIS Building) N-21, W-10, Kita-ku, Sapporo, 001-0021, Japan

    Hiroki Ishihara, Goh Matsuo & Kaoru Tsujii

  2. Graduate School of Science, Hokkaido University, N-10, W-8, Kita-ku, Sapporo, 060-0810, Japan

    Hiroki Ishihara

  3. Faculty of Advanced Life Science, Hokkaido University, Sapporo, 060-0810, Japan

    Takanori Sasaki, Yuko Saito & Makoto Demura

Authors
  1. Hiroki Ishihara
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  2. Goh Matsuo
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  3. Takanori Sasaki
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  4. Yuko Saito
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  5. Makoto Demura
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  6. Kaoru Tsujii
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Corresponding author

Correspondence to Kaoru Tsujii .

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Editors and Affiliations

  1. Graduate School of Sciences Dept. Physics, Kyushu University, 6-10-1 Hakozaki, Fukuoka, 812-8581, Japan

    Masayuki Tokita

  2. Graduate School of Human Life Sciences Dept. Food and Human Health Sciences, Osaka City University, 3-3-138 Sugimoto, Osaka, 558-5858, Japan

    Katsuyoshi Nishinari

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© 2009 Springer-Verlag Berlin Heidelberg

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Ishihara, H., Matsuo, G., Sasaki, T., Saito, Y., Demura, M., Tsujii, K. (2009). Electrophoresis of Dyes and Proteins in Poly(Acrylamide) Gel Containing Immobilized Bilayer Membranes. In: Tokita, M., Nishinari, K. (eds) Gels: Structures, Properties, and Functions. Progress in Colloid and Polymer Science, vol 136. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-00865-8_20

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