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Dicer Inactivation Technology

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MicroRNA Interference Technologies

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Abstract

Targeting miRNAs via interrupting the miRNA biogenesis pathway has become an important alternative to directly targeting mature miRNAs. In theory, every single one of the components along the miRNA biogenesis could be manipulated to disrupt the pathway. Dicer, which is critical for processing of pre-miRNAs into their mature form, has been a popular target helping to assess the global requirement for miRNAs in mammalian biology. Numerous studies using the Dicer inactivation approach have been documented. While this approach provides an invaluable means of studying the global requirement of miRNA for certain biological and pathophysiological processes, it has its inherent limitations. The Dicer inactivation technology is in general a miRNA-loss-of-function strategy; nonetheless, as will be pointed out, it can give rise to paradoxical gain-of-function outcomes.

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Authors and Affiliations

  1. Montreal Heart Institute, Research Center, 5000 Belanger Street, Montreal, QC, H1T 1C8, Canada

    Dr. Zhiguo Wang

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  1. Dr. Zhiguo Wang
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Correspondence to Zhiguo Wang .

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© 2009 Springer-Verlag Berlin Heidelberg

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Wang, Z. (2009). Dicer Inactivation Technology. In: MicroRNA Interference Technologies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-00489-6_13

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