Abstract
This chapter describes the technique of ion net flux measurement outside tissues using ion-selective microelectrodes. This technique is non-destructive, highly sensitive and precise. It allows the recording of net ion fluxes at low external concentrations for several hours at known spots of the root. The theory and the making-of behind the microelectrodes are first given in detail. Then, the equipment needed to measure ion fluxes at the root surface is described, together with a detailed presentation of the factors affecting the flux calculation. In the last part of the chapter, examples of measurements obtained in ectomycorrhizal root systems are given, which demonstrate that this technique could be a really powerful methodology for assessing the actual role of ectomycorrhizal roots in highly heterogeneous root systems of woody plants.
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References
Ammann D (1986) Ion-selective microelectrodes. Principles, design and application. Springer, Berlin Heidelberg, p 346
Gobert A, Plassard C (2002) Differential NO3 −-dependent patterns of NO3 − uptake in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association. New Phytol 154:509–516
Gobert A, Plassard C (2007) Kinetics of NO3 − net fluxes in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association, as affected by the presence of NO3 − and NH4 +. Plant Cell Environ 30:1309–1319
Henriksen GH, Bloom AJ, Spanswick RM (1990) Measurement of net fluxes of ammonium and nitrate at the surface of barley roots using ion-selective microelectrodes. Plant Physiol 93:271–280
Henriksen GH, Raman DR, Walker LP, Spanswick RM (1992) Measurement of net fluxes of ammonium and nitrate at the surface of barley roots using ion-selective microelectrodes: II. Patterns of uptake along the root axis and evaluation of the Microelectrode Flux Estimation Technique. Plant Physiol 99:734–747
Huang JW, Shaff JE, Grunes DL, Kochian LV (1992) Aluminum effects on calcium fluxes at the root apex of aluminum-tolerant and aluminum-sensitive wheat cultivars. Plant Physiol 98:230–237
Kang TM, Markin VS, Hilgemann DW (2003) Ion fluxes in giant excised cardiac membrane patches detected and quantified with ion-selective microelectrodes. J Gen Physiol 121:325–47
Kochian LV, Shaff JE, Lucas WJ (1989) High affinity K uptake in maize roots: a lack of coupling with H+ efflux. Plant Physiol 91:1202–1211
McClure PR, Kochian LV, Spanswick RM, Shaff JE (1990) Evidence for cotransport of nitrate and protons in Maize roots: II. Measurement of NO3 − and H+ fluxes with ion-selective microelectrodes. Plant Physiol 93:290–294
Miller AJ, Cookson SJ, Smith SJ, Wells DM (2001) The use of microelectrodes to investigate compartmentation and the transport of metabolized inorganic ions in plants. J Exp Bot 52:541–549
Newman IA (2001) Ion transport in roots: measurement of fluxes using ion-selective microelectrodes to characterize transporter function. Plant Cell Environ 24:1–14
Newman IA, Kochian LV, Grusak MA, Lucas WJ (1987) Fluxes of H+ and K+ in corn roots: characterization and stoichiometries using ion-selective microelectrodes. Plant Physiol 84:1177–1184
Plassard C, Guerin-Laguette A, Very AA, Casarin V, Thibault JB (2002) Local measurements of nitrate and potassium fluxes along roots of maritime pine. Effects of ectomycorrhizal symbiosis. Plant Cell Environ 25:75–84
Shabala S, Newman I, Morris J (1997) Oscillations in H+ and Ca2+ ion fluxes around the elongation region of corn roots and effects of external pH. Plant Physiol 113:111–118
Shabala L, Ross T, Newman I, McMeekin T, Shabala S (2001) Measurements of net fluxes and extracellular changes of H+, Ca2+, K+, and NH4 + in Escherichia coli using ion-selective microelectrodes. J Microbiol Methods 46:119–29
Thain JF (1995) Electrophysiology. Methods Cell Biol 49:259–274
Wang JJ, Bishop PL (2005) Development of a phosphate ion-selective microelectrode and its use in studies of the enhanced biological phosphorus removal (EBPR) process. Environ Technol 26:381–388
Zou Z, Han J, Jang A, Bishop P, Ahn C (2007) A disposable on-chip phosphate sensor with planar cobalt microelectrodes on polymer substrate. Biosens Bioelectron 22:1902–1907
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© 2009 Springer-Verlag Berlin Heidelberg
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Gobert, A., Plassard, C. (2009). Measurement of Net Ion Fluxes Using Ion-Selective Microelectrodes at the Surface of Ectomycorrhizal Roots. In: Varma, A., Kharkwal, A.C. (eds) Symbiotic Fungi. Soil Biology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95894-9_5
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DOI: https://doi.org/10.1007/978-3-540-95894-9_5
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