Simulation of tissue differentiation in a mechanically loaded bone regeneration chamber

  • Hanifeh KhayyeriEmail author
  • S. Checa
  • M. Tagil
  • P. J. Prendergast
Part of the IFMBE Proceedings book series (IFMBE, volume 22)


Tissue differentiation can be regulated by mechanical loading. A bone chamber experiment has been conducted in order to investigate the effect of loading on tissue differentiation in vivo. In this study a computational model of the experiment was developed in which a mechano-regulation algorithm for stem cell differentiation in response to mechanical stimulus was combined with algorithms for cell migration, proliferation and apoptosis. The results show that the simulation is feasible to forecast the tissue differentiation process inside a bone chamber.


tissue differentiation bone chamber mechano-regulation finite element model numerical simulation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Goodship AE, Kenwright J (1985) The influence of induced micro-movement upon the healing of experimental tibial fractures. J. Bone Joint Surg. Br.Google Scholar
  2. 2.
    de Rooji PP, Siebrecht MAN, Tagil M, Aspenberg P (2001) The fate of mechanically induced cartilage in an unloaded environment. J. Biomech. 34:961–966CrossRefGoogle Scholar
  3. 3.
    Prendergast PJ, Huiskes R, Soballe (1997) Biophysical stimuli on cells during tissue differentiation at implant interfaces. J. Biomech 30: 539–548CrossRefGoogle Scholar
  4. 4.
    Byrne DP, Lacroix D, Planell A, Kelly DJ, Prendergast PJ (2007) Simulation of tissue differentiation in a scaffold as a function of porosity, Young’s modulus and dissolution rate: Application of mechanobiological models in tissue engineering. Biomaterials 28:5544–5554CrossRefGoogle Scholar
  5. 5.
    Perez MA, Prendergast PJ (2007) Random-walk models of cell dispersal included in mechanobiological simulations of tissue differentiation. J. Biomech 40:2244–2253CrossRefGoogle Scholar
  6. 6.
    Grinnel F (1994) Fibroblasts, myofibroblasts, and wound contraction. J. Cell Biol 124:401–404CrossRefGoogle Scholar
  7. 7.
    Tamariz E, Grinnel F (2002) Modulation of fibroblast morphology and adhesion during collagen matrix remodeling. Mol Biol Cell. 13:3915–3929CrossRefGoogle Scholar
  8. 8.
    Appeddu PA, Shur BD (1994) Molecular analysis of cell surface β-1,4-galactosyltransferase function during cell migration. Cell Biol. 91:2095–2099Google Scholar
  9. 9.
    Isaksson H, van Donkelaar CC, Huiskes R, Ito K (2008) A mechano-regulatory bone healing model incorporating cell phenotype specific activity. J. Theor. Biol 252:230–246CrossRefGoogle Scholar
  10. 10.
    Lacroix D, Prendergast PJ (2002) A mechano-regualatory model for tissue differentiation during fracture healing: analysis of gap size and loading. J. Biomech. 35:1163–1171CrossRefGoogle Scholar
  11. 11.
    Lacroix D, Prendergast PJ, Li G Marsh D (2002) Biomechanical model to simulate tissue differentiation and bone regeneration: application to fracture healing. Med. Biol. Eng Comput 40:14–21CrossRefGoogle Scholar
  12. 12.
    Claes LE, Heigele CA (1999) Magnitudes of local stress and strain along bony surfaces predict the course and type of fracture healing. J. Biomech. 32:255–266CrossRefGoogle Scholar
  13. 13.
    Geris L, Vandamme K, Naert I, Sloten JV, Duyck J, Oosterwyck HV (2008) Application of mechanoregulatory models to simulate periimplant tissue formation in an in vivo bone chamber. J. Biomech 41:145–154CrossRefGoogle Scholar
  14. 14.
    Isaksson H, van Donkelaar CC, Huiskes R, Ito K (2006) Corroboration of mechanoregulatory algorithms for tissue differentiation during fracture healing: Comparison with in vivo results. J. Orthop. Res. 24:898–907CrossRefGoogle Scholar
  15. 15.
    Carter DR, Blenman PR, Beaupre GS (1988) Correlations between mechanical stress history and tissue differentiation in initial fracture healing. J. Orthop. Res. 6:736–748CrossRefGoogle Scholar
  16. 16.
    Fisher JP, Mikos AG, Bronzino JD (2007) Tissue Engineering. Taylor & Francis, New YorkCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Hanifeh Khayyeri
    • 1
    Email author
  • S. Checa
    • 1
  • M. Tagil
    • 2
  • P. J. Prendergast
    • 1
  1. 1.Trinity Centre for BioengineeringSchool of Engineering, Trinity CollegeDublinIreland
  2. 2.Department of OrthopedicsLund University HospitalLundSweden

Personalised recommendations