The Perils of Polynucleotides Revisited

  • Nadrian C. SeemanEmail author
Part of the Natural Computing Series book series (NCS)


DNA computing relies on the successful implementation of physical chemistry techniques involving oligonucleotides of prescribed sequence. Our laboratory has been involved in the assembly and manipulation of designed oligonucleotides in order to pursue studies in genetic recombination and nanofabrication. We have constructed a large number of unusual branched DNA motifs used to build a variety of DNA objects, lattices, and nanomechanical devices. Our experience with these systems has uncovered a large number of experimental pitfalls that may confront individuals working with DNA-based computation. A decade ago, we presented our experience in this area in the hope that we could help investigators to anticipate experimental problems that affect DNA computing schemes. Here, we review these points from the vantage point of further experience, indicating both modifications to the original criteria and new points as well.


Major Groove Nucleotide Pair Branch Migration Sequence Symmetry Nanomechanical Device 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  1. 1.Department of ChemistryNew York UniversityNew YorkUSA

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