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Nanobiotechnology: Soft Lithography

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Biosilica in Evolution, Morphogenesis, and Nanobiotechnology

Part of the book series: Progress in Molecular and Subcellular Biology ((MMB,volume 47))

Abstract

An entirely new scientific and technological area has been born from the combination of nanotechnology and biology: nanobiotechnology. Such a field is primed especially by the strong potential synergy enabled by the integration of technologies, protocols, and investigation methods, since, while biomolecules represent functional nanosystems interesting for nanotechnology, micro- and nano-devices can be very useful instruments for studying biological materials. In particular, the research of new approaches for manipulating matter and fabricating structures with micrometre- and sub-micrometre resolution has determined the development of soft lithography, a new set of non-photolithographic patterning techniques applied to the realization of selective proteins and cells attachment, microfluidic circuits for protein and DNA chips, and 3D scaffolds for tissue engineering. Today, soft lithographies have become an asset of nanobiotechnology. This Chapter examines the biological applications of various soft lithographic techniques, with particular attention to the main general features of soft lithography and of materials commonly employed with these methods. We present approaches particularly suitable for biological materials, such as microcontact printing (μCP) and microfluidic lithography, and some key micro- and nanobiotechnology applications, such as the patterning of protein and DNA microarrays and the realization of microfluidic-based analytical devices.

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Authors and Affiliations

  1. National Nanotechnology Laboratory of Istituto Nazionale di Fisica della Materia-Consiglio Nazionale delle Ricerche, Distretto Tecnologico ISUFI, Università degli Studi di Lecce, via Arnesano, Lecce, I-73100, Italy

    Elisa Mele & Dario Pisignano

Authors
  1. Elisa Mele
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  2. Dario Pisignano
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Editors and Affiliations

  1. Inst. Physiologische Chemie Abt. Angewandte Molekularbiologie, Universität Mainz, Duesbergweg 6, Mainz, 55099, Germany

    Werner E. G. Müller

  2. Siberian Branch, Limnological Institute, Russian Academy of Sciences, Ulan-Batorskaya st. 3, Irkutsk, 664033, Russia

    Mikhael A. Grachev

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© 2009 Springer-Verlag Berlin Heidelberg

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Mele, E., Pisignano, D. (2009). Nanobiotechnology: Soft Lithography. In: Müller, W.E.G., Grachev, M.A. (eds) Biosilica in Evolution, Morphogenesis, and Nanobiotechnology. Progress in Molecular and Subcellular Biology, vol 47. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-88552-8_15

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