Abstract
Human bone marrow MSCs represents a phenotypically homogeneous cell population that share an identical phenotype with marrow adventitial reticular cells. When an extensive panel of markers is used to characterize MSCs, it appears that the diverse MSC markers described in different laboratories are expressed on the same cell population. Although investigators speak of a number of specific MSC markers, a true marker of MSC “stemness” and multipotentiality has not yet been defined since culture-expanded MSCs may lose some of these markers, but remain multipotential. This chapter will introduce the criteria and some comments on human MSCs defined by International Society for Cellular Therapy (ISCT).
Mesenchymal stem cells (MSCs) are multipotent progenitor cells (Horwitz et al, 2005). During the last two decades, although increasing interest has occurred in the research field of MSCs, different researchers have considered the defining characteristics of MSCs differently. The different methods of MSCs isolation and expansion make it difficult to compare the results of these studies.
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References
Barry FP, Boynton RE, Haynesworth S, Murphy JM, Zaia J (1999) The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105). Biochem Biophys Res Commun, 265(1):134–139
Barry F, Boynton R, Murphy M, Haynesworth S, Zaia J (2001) The SH-3 and SH-4 antibodies recognize distinct epitopes on CD73 from human mesenchymal stem cells. Biochem Biophys Res Commun, 289(2):519–524
Bruder SP, Horowitz MC, Mosca JD, Haynesworth SE (1997) Monoclonal antibodies reactive with human osteogenic cell surface antigens. Bone, 21(3):225–235
Bruder SP, Kraus KH, Goldberg VM, Kadiyala S (1998) The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects. J Bone Joint Surg Am, 80(7):985–996
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 8(4):315–317
Gang EJ, Bosnakovski D, Figueiredo CA, Visser JW, Perlingeiro RC (2007) SSEA-4 identifies mesenchymal stem cells from bone marrow. Blood, 109(4): 1743–1751
Haynesworth SE, Baber MA, Caplan AI (1992) Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone, 13(1):69–80
Horwitz EM, Le Blanc K, Dominici M, Mueller I, Slaper-Cortenbach I, Marini FC, Deans RJ, Krause DS, Keating A (2005) Clarification of the nomenclature for MSC. The International Society for Cellular Therapy position statement. Cytotherapy, 7(5):393–395
Lagar’kova MA, Lyakisheva AV, Filonenko ES, Volchkov PY, Rubtsova KV, Gerasimov YV, Chailakhyan RK, Kiselev SL (2006) Characteristics of human bone marrow mesenchymal stem cells isolated by immunomagnetic selection. Bull Exp Biol Med, 141(1):112–116
Martinez C, Hofmann TJ, Marino R, Dominici M, Horwitz EM (2007) Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs. Blood, 109(10):4245–4248
Mets T, Verdonk G (1981) In vitro aging of human bone marrow derived stromal cells. Mech Ageing Dev, 16(1):81–89
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© 2009 Zhejiang University Press, Hangzhou and Springer-Verlag GmbH Berlin
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Hu, X. (2009). MSCs Identification. In: Wang, J., Xie, X. (eds) Mesenchymal Stem Cells for the Heart. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-88150-6_2
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DOI: https://doi.org/10.1007/978-3-540-88150-6_2
Publisher Name: Springer, Berlin, Heidelberg
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